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Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS)

By Li Yi-Xue, Zhang Xue-Gong, Li Ting-Ting, Wang Yun, Wang Xiao-Wo, Hong Yi, Deng Qing, Deng Fu-Xing, Zhang Yun-Li, Hao Pei, Huang Jian, Yang Peng-Yuan, Wang Hong-Yang and Han Ze-Guang


<p>Abstract</p> <p>Background</p> <p>The liver is the largest human internal organ – it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which account for three-quarters of the liver's volume, perform its main functions. To discover the molecular basis of hepatocyte function, we employed Massively Parallel Signature Sequencing (MPSS) to determine the transcriptomic profile of adult human hepatocytes obtained by laser capture microdissection (LCM).</p> <p>Results</p> <p>10,279 UniGene clusters, representing 7,475 known genes, were detected in human hepatocytes. In addition, 1,819 unique MPSS signatures matching the antisense strand of 1,605 non-redundant UniGene clusters (such as <it>APOC1</it>, <it>APOC2</it>, <it>APOB </it>and <it>APOH</it>) were highly expressed in hepatocytes.</p> <p>Conclusion</p> <p>Apart from a large number of protein-coding genes, some of the antisense transcripts expressed in hepatocytes could play important roles in transcriptional interference via a <it>cis</it>-/<it>trans</it>-regulation mechanism. Our result provided a comprehensively transcriptomic atlas of human hepatocytes using MPSS technique, which could be served as an available resource for an in-depth understanding of human liver biology and diseases.</p

Topics: Genetics, QH426-470, Biology (General), QH301-705.5, Science, Q, DOAJ:Genetics, DOAJ:Biology, DOAJ:Biology and Life Sciences, Biotechnology, TP248.13-248.65
Publisher: BioMed Central
Year: 2007
DOI identifier: 10.1186/1471-2164-8-207
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