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Effect of alcohol and formaldehyde on the ä13C and ä15N isotopic composition of Plagioscion squamosissimus and Hypophthalmus edentatus (Pisces, Osteichthyes) = Efeito do alcool e formol sobre a composicao isotopica de ƒÂ13C e de ƒÂ15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes)

By Gislaine Iachstel Manetta, Evanilde Benedito and Carlos Ducatti

Abstract

The present study investigates the ƒÂ13C and ƒÂ15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze . control group (-15oC), conservation in alcohol 70%and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for ƒÂ13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish.<br><br>O presente estudo investiga a composicao isotopica de ƒÂ13C e ƒÂ15N entre as amostras congeladas (controle), em alcool e em formol de Plagioscion squamosissimus e Hypophthalmus edentatus. De cada individuo foi extraida uma faixa de musculo localizada na regiao acima da linha lateral, na base da nadadeira dorsal, a qual foi subdividida em tres partes iguais, sendo cada uma delas submetida a um tipo de tratamento: congelamento - grupo control (-15oC), conservacao em alcool 70% e fixacao em formol 4%. As amostras foram mantidas nos referidos tratamentos por 30 dias, enxaguadas e submersas em agua destilada por 4h. Em seguida, foram secas em estufa de ventilacao a 60oC por 48h e maceradas ate a obtencao de po. Identificou-se diferenca significativa nos valores isotopicos de carbono e de nitrogenio, entre o controle e as amostras de alcool e as de formol, com excecao do ƒÂ13C das amostras mantidas em formol de H. edentatus. Constatou-se que os valores isotopicos de carbono das amostras conservadas em alcool foram, na sua maioria, enriquecidos quando comparados com as controle, ao passo que, as amostras em formol tiveram os valores deplecionados em relacao ao controle. Os valores isotopicos de nitrogenio, tanto para as amostras conservadas em alcool, quanto em formol, foram enriquecidos quando comparados aos valores daquelas congeladas, sendo estes independentes dos conservantes utilizados. Assim, a correcao isotopica deve ser realizada de acordo com o isotopo e o conservante empregado para especies de peixe de agua doce

Topics: stable isotope, preservative, fishes, Plagioscion squamosissimus, Hypophthalmus edentatus, isótopos estáveis, conservantes, peixes, Biology (General), QH301-705.5, Science, Q, DOAJ:Biology, DOAJ:Biology and Life Sciences, Microbiology, QR1-502
Publisher: Eduem - Editora da Universidade Estadual de Maringá
Year: 2011
OAI identifier: oai:doaj.org/article:1b62e05b27644944935a97813c80ef3e
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