In this work were analysed samples of blood from 314 dairy cows Slovak spotted breed by the method of starch gel electrophoresis. The aim was to identify the biochemical polymorphic markers in blood plasma and in extract of erythrocytes. The polymorphism of hemoglobin (AA+AB+BB), amylasa (AA+AB+BB), transferrin (AA+AD1+AD2+AE+D1D1+D1E), albumin (AA+AB+BB) and postalbumin (AA+AB+AC+BB+BC+CC) were proved. File of dairy cows was divided to fi ve groups by age. The fi rst group create of dairy cows in age 25-48 months, second 49-72 months, third 73-96 months, fourth 97-120 months and the last over 121 months. According to evaluation of heterozygotes genotypes were detect decrease into of AB genotype in polymorphism of hemoglobin from primary 46.8 % (25-48 month) to 11.1 % in the last age group of dairy cows up 121-th months. Heterozygotic combinations in transferrin locus (AD1+AD2+AE+D1E genotypes) culminated from the beginning 14.2 % to 38.9 % at the fi rst group and last one. The postalbumin polymorphism had the high rate of heterozygotes because of AB+AC+BC genotypes. Rate of heterozygous genotypes AB in the polymorphism system amylasa variance between 15.4 - 23.7 %. Heterozygotic genotypes AB in albumin locus was according to our analyse at dairy cows in the fi rst evaluated of age group 52.9 %, second one only in fi fth groups at intervals 71.8 - 75.0 %. Among tested heterozygotes (AB genotype) and homozygotes (AA+BB genotypes) we found the signifi cant differences only in albumin locus 2,18+ (+P≤0,05)
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