The objective of this work was to study the spatial variability of soil microbial biomass (SMB) and labile soil organic matter pools (labile SOM), under different management systems and plant cover. The experiment was conducted in a Haplic Planosol soil on an Integrated Agroecological Production System (SIPA), in Seropédica, Rio de Janeiro. The evaluated management systems were: alley cropping, pasture, and bush garden, the late one was used as reference area. Three grids of regular spacing of 2.5 x 2.5 meters were used for sampling, consisting of 25 georeferenced points each, where soil samples were taken at 0-10 cm depth. The following labile constituents of soil organic matter were determined: free light fraction (FLF), water soluble C and N, C and N of SMB (SMB-C and SMB-N), and glomalin content. The textural fractions (sand, silt, and clay), pH in water, and chemical attributes (organic C, total N, Ca, Mg, Al, P, K, and CEC-cation exchange capacity) were also determined. The areas of alley cropping and pasture showed spatial dependence to the attributes of SOM. The occurrence of high spatial dependence for the attributes associated to microbial biomass in the alley cropping system (C, FLF, SMB-N and respiration), probably was due to external factors related to management, such as: intensive rotational cropping system, diversity of crops and different inputs of organic matter to soil such as pruning material and organic compost
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