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Protein expression of VEGF, IGF-1 and FGF in retroocular connective tissues and clinical correlation in Graves' ophthalmopathy Expressão protéica de VEGF, IGF-1 e FGF no tecido conjuntivo retro-ocular e correlação clínica na oftalmopatia de Graves

By Kimble Matos, Paulo Gois Manso, Eduardo Marback, Reinaldo Furlanetto, Gustave Nosé Alberti and Vania Nosé

Abstract

PURPOSE: To investigate the immunohistochemical expression (IGF-1, EGFr, EGF, c-erbB-2/HER-2/neu, PDGF-A, PDGF-B, FGF and VEGF) in patients with Graves' ophthalmopathy. METHODS: Twenty-four samples (Graves' ophthalmopathy patients) underwent lateral rectus muscle and surrounding fibrous and adipose tissue biopsy. The control group was obtained by strabismus surgery. Correlation between clinical- ophthalmologic, endocrinological, ultrasonographic findings, and immunohistochemical expression was performed. RESULTS: IGF-1: There were 7 positive cases (29.2%). There was a direct relation with higher CAS (clinical activity score) in all of them and if only CAS equal or higher than 5 was considered, this was 54.5%. FGF: There was expression in 5 cases (20.8%) with a direct relation in all those with higher CAS (>5) (45.4%). VEGF: There were two positive cases (8.3%) for VEGF in endothelial cells, in these cases the patients also presented CAS higher than 5. There was no expressions of all growth factors in the control group. CONCLUSIONS: All patients, except one, with positive expression of FGF, IGF-1 and VEGF showed CAS greater than 5, suggesting in this way an important role of these growth factors in the pathogenesis and severity of Graves' ophthalmopathy. However, statistical analysis revealed only significant association between IGF-1 and male sex (P=0.034). Low ultrasound reflectivity and endocrine status may not correlate directly with disease activity or with immunoexpression of growth factors and c-erbB-2/HER-2/neu.<br>OBJETIVO: Investigar a expressão imuno-histoquímica de IGF-1, EGFr, EGF, c-erbB-2/HER-2/neu, PDGF-A, PDGF-B, FGF e VEGF na oftalmopatia de Graves. MÉTODOS: Vinte e dois pacientes (oftalmopatia de Graves) foram submetidos à biópsia do músculo reto lateral e tecido fibroso e adiposo adjacente. O grupo controle foi de pacientes de cirurgia de estrabismo. Foi feita correlação entre achados clínico-oftalmológicos, endocrinológicos, ultra-sonográficos e da expressão imuno-histoquímica dos fatores de crescimento. RESULTADOS: IGF-1: Houve 7 casos positivos (29,2%). Houve correlação direta com o CAS (clinical activity score) elevado em todos os casos e em que consideramos CAS apenas acima de 5, em 54,5%. FGF: Houve expressão em 5 casos (20,8%) com relação direta com CAS elevado em todos os casos e em que consideramos CAS maior que 5 (45,4%). VEGF: Houve dois casos positivos (8,3%) para VEGF nas células endoteliais e estes casos também apresentavam CAS maior que 5. A imunorreatividade foi negativa em todo grupo controle. CONCLUSÃO: Todos os pacientes, com exceção de um, com expressão positiva para FGF, IGF-1 e VEGF mostraram CAS maior que 5, sugerindo importante papel destes fatores de crescimento na patogênese e gravidade da oftalmopatia de Graves. Entretanto, a análise estatística demonstrou associação significativa entre IGF-1 e o sexo masculino (P=0,034). Baixa refletividade ao ultra-som e condição endócrina não estiveram correlacionadas

Topics: Imuno-histoquímica, Receptor erbB-2, Fator de crescimento insulin-like I, Receptor do fator de crescimento epidérmico, Fator de crescimento epidérmico, Receptor tipo alfa para fator de crescimento derivado de plaqueta, Proteínas proto-oncogênicas c-sis, Fatores de crescimento de fibroblastos, Fator A de crescimento do endotélio vascular, Oftalmopatia de Graves, Imunohistochemstry, Receptor, erbB-2, Insulin-like growth factor I, Receptor, epidermal growth factor, Epidermal growth factor, Platelet-derived growth factor alpha, Proto-oncogene proteins c-sis, Fibroblast growth factors, Vascular endothelial growth factor A, Graves' ophthalmopathy, Ophthalmology, RE1-994, Medicine, R, DOAJ:Ophthalmology, DOAJ:Medicine (General), DOAJ:Health Sciences
Publisher: Conselho Brasileiro de Oftalmologia
Year: 2008
DOI identifier: 10.1590/S0004-27492008000400004
OAI identifier: oai:doaj.org/article:411f1dc639be4005b06d3e32cd768fc0
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