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Utilização de um teste de ELISA polivalente para detecção de anticorpos contra Actinobacillus pleuropneumoniae (App) Utilization of a polyvalent ELISA test for detection antibodies against of Actinobacillus pleuropneumoniae

By J.D. Kich, I.A. Piffer, A.L. Guidoni, D.E.S.N. Barcellos and C.S. Klein

Abstract

Um teste de ELISA polivalente baseado em lipopolissacarídeos de cadeia longa (LPS - CL) de Actinobacillus pleuropneumoniae (App) sorotipos 3, 5 e 7 foi avaliado testando-se amostras do soro de leitões e matrizes provenientes de 10 rebanhos positivos e de 10 rebanhos negativos. Foram classificados como positivos aqueles rebanhos com isolamento prévio do App sorotipos 3, 5 ou 7 e rebanhos negativos aqueles submetidos ao controle veterinário, sem notificação de sintomas clínicos, sem lesões de pleuropneumonia suína e sem isolamento do agente. Todos os rebanhos positivos apresentaram sorologia positiva e as matrizes apresentaram maior número de soroconversores (P<0,05) do que os leitões. Entre os rebanhos negativos quatro apresentaram sorologia negativa, cinco sorologia positiva com valores preditivos altos (96 a 99%) e um com valor preditivo considerado baixo (56%). O teste apresentou 100% de sensibilidade e aparentemente baixa especificidade, porém, como detectou os sorotipos prevalentes no Brasil e sorotipos que possuem LPS - CL homólogos (3, 4, 5, 6, 7 e 8), ele é aplicável somente como teste de triagem.<br>Sera from piglets and sows from 10 positive and 10 negative herds to Actinobacillus pleuropneumoniae (App) were submitted to a polyvalent ELISA test based on long chain lipopolysaccharides (LC-LPS) against sorotypes 3, 5 and 7. Positive herds were those in which a strain of serotype 3, 5 or 7 was isolated and negative those ones in which there were neither notification of clinical symptoms, nor lesions of swine pleuropneumoniae, nor App isolation and all the herds were under veterinary control. All positive herds contained animals that seroconverted and the frequency of sows serologically positive was significantly higher than that of positive piglets. Four negative herds were serologically negative, but five herds had sows considered positive with high positive predictive values (96 to 99%) and one herd had a positive predictive value of 56%. The polyvalent ELISA LC-LPS to App serotypes 3, 5 and 7 had 100 % of sensibility but apparently low specificity. The sensibility of the polyvalent test and its capability of detecting the prevalent Brazilian serotypes and those with shared LPS antigens (3, 4, 5, 6, 7 e 8) indicates that this test might be used only as a screening test

Topics: Suínos, Actinobacillus pleuropneumoniae, pleuropneumonia, ELISA, Swine, Actinobacillus pleuropneumoniae, pleuropneumonia, ELISA, Zoology, QL1-991, Science, Q, DOAJ:Zoology, DOAJ:Biology, DOAJ:Biology and Life Sciences, Animal culture, SF1-1100, Agriculture, S, DOAJ:Animal Sciences, DOAJ:Agriculture and Food Sciences
Publisher: Universidade Federal de Minas Gerais
Year: 1999
DOI identifier: 10.1590/S0102-09351999000500002
OAI identifier: oai:doaj.org/article:50dc61f3fd0149efb8f0723312dfe5f0
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