Location of Repository

Substrates used in SIR assays can inhibit basal respiration in rewetted soil

By K.M. Bialkowski, R. Archibald, G.E.St.J. Hardy and T.I. Burgess

Abstract

Respiration assays are routinely used for investigating microbial metabolic activity in soil, but usually after a period of "conditioning" whereby dry soil is rewetted and incubated for a period of days. We showed that rewetting and incubation of soil with or without amendments cause changes in microbial populations that are dependent on the type of amendment. As these amendments resulted in altered basal respiration levels and SIR profiles, they call into question the suitability of soil conditioning as pretreatment for soil microbial analyses. When testing soils from an experiment involving various amendments we have found that different substances can inhibit, rather than stimulate, respiration following rewetting. We suggest further investigation of "CO2 burst inhibition" for the purpose of developing a method that does not require naturally dry or air dried soil to undergo conditioning prior to a SIR assay

Year: 2010
OAI identifier: oai:researchrepository.murdoch.edu.au:15513
Provided by: Research Repository

Suggested articles

Preview

Citations

  1. (2010). 19th World Congress of Soil Science, Soil Solutions for a Changing World
  2. (2003). A proposed mechanism for the pulse in carbon dioxide production commonly observed following the rapid rewetting of a dry soil.
  3. (2003). A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil.
  4. (2007). Assessing CLPPs using MicroReSp (TM) -A comparison with biolog and multi-SIR.
  5. (2007). Comparison of two methods that assess soil community level physiological profiles in a forest ecosystem.
  6. (2002). Experimental design and data analysis for biologists'
  7. (1998). Microbial functional diversity can be influenced by the addition of simple organic substrates to soil.
  8. (2006). Miniaturized test system for soil respiration induced by volatile pollutants.
  9. (1978). Physiological method for quantitative measurement of microbial biomass in soils.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.