The main aim of this phd thesis is the definition of a methodological framework for the determination, analysis and cross-evaluation of urban clusters which are formulated within wider study areas, such as administrative regions. To this end, different methods and techniques were combined, that stem from the fields of Statistics and Quantitative Spatial Analysis and which during the last years are all the more commonly applied to the different stages of the Geographical Analysis process. In this regard, there are four interconnecting components that form the proposed methodology. Cluster Analysis, Distance Counts, Service Areas and Spatial Autocorrelation. Furthermore, the definition of urban clusters is mainly based on different types of data concerning the demographic characteristics of cities, the number of public facilities located, as well as the classification of the existing road network in the study area. The approach was assessed through its application to the Region of Thessaly, Greece.