The present study aimed at the standardization of the motor subscales (locomotor and fine motor) of the Griffiths Test No II on preschool-aged children. A fundamental precondition for the application of a developmental test is its standardization on the population of the country where it is to be applied, so that one can determine, for every item, the developmental ages which represent psychomotor development. In order to standardize the A-locomotor and D-fine motor - or hand and eye coordination - of the Griffiths Test No II, 930 children were assessed (455 boys and 475 girls), aged from 37 to 72 months (3-6 years old). The children were selected from various areas of Northern Greece and from all socioeconomic sections of society, so that the sample was representative. Following the Griffiths Test standards, for each one of the three age groups - 3-4, 4-5, and 5-6 years old - a sufficient number of children that would represent each group (272, 326, and 332 respectively) was assessed. Based on each subscale, the developmental age, and then the developmental quotient could be identified. Therefore, there were two individual developmental quotients for every child (subscale A and subscale D). Through the statistical analysis of the results, the initial, middle and marginal age for each item of the subscales A and D was determined, as well as the percentage positions a) depending on the developmental quotient and the growth age of the child, and b) depending on the successful items and the growth age of the child. Thus, the Physical Educator can determine, through an easy method, the motor developmental condition. This is very important, because the motor development (locomotor and fine motor) of the child will determine the educational program based on which the child will be further educated. Moreover, the teacher can evaluate the visual and visual-motor perception of the child, which constitute fundamental elements for the development of mature movement. Through the present application of the Griffiths Test to the children in our country, we were able to realize that the average developmental quotients and the two subscales were higher compared to the relevant quotients of the Griffiths Test. This means that the children of the present study execute earlier, that is, at a younger age, most of the items included in the two motor subscales of the test. Some of the factors that can influence the faster development of the motor development are the following: the passage of time, which has a positive effect on the physique and the psychomotor development, regardless of the country where the child is growing up, the environmental and cultural differences among the countries, and the enrichment of the curriculum with more complicated and challenging activities, which would result in the better education and development of the child. According to the results of the study, we can conclude that the motor subscales of the Griffiths test offer the possibility for an objective evaluation of the motor condition, the rate of motor development, and the timely detection of a dormant deviation, without the need for time-consuming or special instruments. Moreover, through the great number of items that the test examines, we can identify the motor level in children with motor difficulties, and monitor the motor development of normal children and children with disadvantages after a therapeutic intervention. Finally, the higher developmental quotients of the children in this study fortify the need for the standardization of a test before it is applied to the population of a country, and its re-standardization on the population of the country where it was first applied.