Ischemiareperfusion lung injury attenuated by ATP-MgCl2 in rats

Abstract

552, 1994.-The protective effect of ATP-MgCl, on ischemia-reperfusion lung injury has been reported in kidney, liver, heart, and muscle but has not been examined in lungs. The aim of this study was to determine whether ATP or ATP-MgCl, pretreatment would attenuate ischemia-reperfusion-induced acute lung injury and to identify the possible mechanisms for such protection. Typical acute lung injury was successfully in-duced in Sprague-Dawley rats by 10 min of hypoxia followed by 75 min of ischemia and 50 min of reperfusion. Pretreatment with ATP-MgCl, (or adenosine) but not ATP or MgC1, (all. at lop6 M) significantly attenuated the acute lung injury. All the protective effects of ATP-MgCl, were nearly undetectable when promazine (an ecto-adenosinetriphosphatase inhibitor) or 3,7-dimethyl-1-propargylxanthine (an AZ-receptor antago-nist) was added before ATP-MgCl, pretreatment. These obser