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MEMBRANE FLUIDITY OF NEONATAL POLYMORPHONUCLEAR CELLS AND OXIDATIVE METABOLISM

By ΜΑΡΙΑ ΚΟΛΙΟΥ-ΜΑΖΕΡΗ

Abstract

THE AIM OF THE PRESENT STUDY WAS TO ASSESS THE MEMBRANE FLUIDITY OF NEONATAL POLYMORPHONUCLEARS (PMNS) AND TO STUDY THE EFFECTS OF FLUIDITY ALTERATIONS TO THE PMNS OXIDATIVE METABOLISM. THE PMNS WE HAVE STUDIED DERIVED FROM VENOUS BLOOD FROM HEALTHY FULLTERM NEONATES, 3-5 DAYS OLD, CORD BLOOD FROM HEALTHY FULLTERM PREGNANCIES AND VENOUS BLOOD FROM HEALTHY ADULTS AS CONTROLS. THE FORMYL PEPTIDE FMLP 10 -7 M WAS USED AS A PMN STIMULUS. EMPLOYING A STEADY-STATE FLUORESCENCE POLARIZATION METHOD WE FOUND THAT THE FLUID STATE OF THE MEMBRANE OFPMNS FROM NEONATES WAS SIGNIFICANTLY LESS THAN THAT OF ADULT CONTROLS. ETHANOLSIGNIFICANTLY INCREASED THE FLUIDITY OF THE CELL MEMBRANES OF NEONATAL PMNS. OXYGEN METABOLISM WAS ASSESSED BY O2 PRODUCTION AND LUMINOL DEPENDENT CHEMILUMINESCENCE (L-CL). CORD BLOOD PMNS PRODUCED DECREASED AMOUNT OF O2 COMPARED TONEONATAL PMNS AND EVEN LESS AMOUNT OF O2 WAS PRODUCED BY ADULT PMNS. ALTHOUGHNEONATAL PMNS PRODUCED MORE O2 THAN THE ADULT PMN, IN HIGH CONCENTRATIONS OF STIMULUS (FMLP 10 -6 M) THE RATE OF INCREMENT OF O2 PRODUCTION IN NEONATAL CELLS WAS MUCH LOWER THAN FROM ADULT PMNS (P < 0.05). NEONATAL PMNS PRODUCED LESSL-CL THAN ADULT PMNS. AFTER THE ADDITION OF ALIPHATIC ALCOHOL NEONATAL PMNS PRODUCED LESS AMOUNT OF O2 AND L-CL. THE ABOVE FINDINGS SUGGEST THAT THE INCREASE OF MEMBRANE FLUIDITY CAUSES A DECREASE IN O2 PRODUCTION AND AN EVEN MORE DECREASE OF THE L-CL WHICH IS ALREADY LOW IN NEONATAL PMNS. (ABSTRACT TRUNCATED)

Topics: Chemiluminescence, MEMBRANE FLUIDITY, Neonates, Neutrophils, OXIDATIVE METABOLISM, STEADY STATE FLUORESCENCE POLARIZATION, SUPEROXIDE PRODUCTION, Ελεύθερες ρίζες οξυγόνου, ΜΕΜΒΡΑΝΙΚΗ ΡΕΥΣΤΟΤΗΤΑ, ΝΕΟΓΝΙΚΟ ΠΟΛΥΜΟΡΦΟΠΥΡΗΝΟ, ΠΟΛΩΜΕΝΗ ΦΘΟΡΙΖΟΥΣΑ ΑΚΤΙΝΟΒΟΛΤΑ, ΡΙΖΑ ΥΠΕΡΟΞΕΙΔΙΟΥ, Χημειοφωταύγεια
Publisher: National and Kapodistrian University of Athens
Year: 1995
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