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ΕΠΙΔΡΑΣΗ ΤΩΝ ΣΤΕΡΟΕΙΔΩΝ ΟΡΜΟΝΩΝ ΣΤΗΝ ΕΚΦΡΑΣΗ ΤΟΥ ΟΓΚΟΝΙΔΙΟΥ C-ERBB2 /NEU ΣΤΑ ΑΔΕΝΟΚΑΡΚΙΝΙΚΑ ΚΥΤΤΑΡΑ ΤΟΥ ΑΝΘΡΩΠΙΝΟΥ ΕΝΔΟΜΗΤΡΙΟΥ (ΚΥΤΤΑΡΙΚΗ ΣΕΙ...

By ΕΜΜΑΝΟΥΗΛ ΜΑΡΚΟΓΙΑΝΝΑΚΗΣ

Abstract

RECENT FINDINGS REGARDING THE MOLECULAR ACTION OF STEROID HORMONES AND THEIR ROLE IN ENDOMETRIAL CELL TRANSFORMATION SUGGEST THAT ESTROGENS ACT AS TUMOR PROMOTERS, PROPAGATING PRE-EXISTING DNA LESIONS IN PROTO-ONCOGENES. ACCORDINGLY, WE HAVE INVESTIGATED THE EFFECTS OF ESTROGENS AND OTHER STEROID HORMONES ON ERBB2 GENE EXPRESSION IN ISHIKAWA HUMAN ENDOMETRIAL ADENOCARCINOMA CELLS. WE HAVE FOUND THAT THE ERBB2/NEU TRANSCRIPT AND ITS PROTEIN PRODUCT ARE PRESENT IN ABUNDANCE, IN THE ISHIKAWA ENDOMETRIAL ADENOCARCINOMA CELL LINE AS WELL AS IN HUMAN ENDOMETRIAL ADENOCARCINOMA CELLS. BOTH CELL TYPES EXPRESS THE 4.6 AND 2.3 KB ERBB2 MRNAS, RESULTING IN THE SYNTHESIS OF THE MEMBRANE 185 KDA AND THE TRUNCATED EXTRACELLULAR 100 KDA PROTEIN. ESTRADIOL SIGNIFICANTLY INCREASEDIN A TIME- AND DOSE-DEPENDENT MANNER THE CONTENT OF ERBB2 MRNA AND THE CONCENTRATION OF NEU PROTEIN IN ISHIKAWA CELL EXTRACTS, WHILE PROGESTERONE WAS DEVOID OF ANY ACTIVITY. THE EFFECT OF ESTRADIOL WAS PARTIALLY REVERSED BY THE ANTIESTROGEN 4-HYDROXYTAMOXIFEN, WHICH, HOWEVER GIVEN ALONE EXHIBITED AGONISTICEFFECTS. GLUCOCORTICOID DEXAMETHASONE AUGMENTED IN A TIME- AND DOSE-DEPENDENT FASHION THE CONTENT OF ERBB2 MRNA AND THE CONCENTRATION OF NEU ISHIKAWA CELL EXTRACTS. THE ANTIGLUCOCORDICOID RU 486 ACTED AS GLUCOCORTICOID AGONIST INCREASING ERBB2 GENE ACTIVATION. TO OUR KNOWLEDGE, THIS IS THE FIRST EXPRESSIONIN NEOPLASTIC HUMAN ENDOMETRIAL CELLS. (ABSTRACT TRUNCATED)

Topics: ERBB2/NEU GENE, Estrogens, Gene regulation, Glucocorticoids, HUMAN ENDOMETRIAL CARCINOMA CELLS, Ishikawa cells, ΑΔΕΝΟΚΑΡΚΙΝΟΜΑ ΕΝΔΟΜΗΤΡΙΟΥ, Γλυκοκορτικοειδή, Γονιδιακή ρύθμιση, ΚΥΤΤΑΡΙΚΗ ΣΕΙΡΑ ISHIKAWA, ΟΓΚΟΓΟΝΙΔΙΟ ERBB2/NEU, Οιστρογόνα
Publisher: University of Crete (UOC)
Year: 1996
DOI identifier: 10.12681/eadd/6029
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