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DEVELOPMENT OF METHODS FOR THE DETERMINATION AND EVALUATION OF SOLID SUPPORTED FUNCTIONAL GROUPS APPROPRIATE FOR COVALENT IMMOBILIZATION OF BIOMOLECULES

By ΦΙΛΗΜΩΝ ΤΥΛΛΙΑΝΑΚΗΣ

Abstract

IN THE PRESENT WORK IS DESCRIBED THE DEVELOPMENT OF METHODS FOR THE COLORIMETRIC DETERMINATION OF: A) TOTAL FUNCTIONAL GROUPS ON THE SURFACE OF SOLID SUPPORTS CARRYING ALDEHYDO-, HYDRAZIDO-, NHS-ESTERS AND SULFHYDRYLO- GROUPS. B) REACTIVE FUNCTIONAL GROUPS ON THE SURFACE OF SOLID SUPPORTS CARRYING AMINO-, CARBOXYL-, ALDEHYDO-, AND NHS-ESTERS GROUPS AND C) MOLECULES SUCH AS THYROXIN, TYROSINE AND CYSTEINE IMMOBILIZED ONTO THE SURFACE OF SOLID CARRIERS. THE METHODS ARE BASED ON THE UTILIZATION OF BICINCHONINIC ACID/CUSO4 SOLUTION AND WERESUCCESSFULLY APPLIED FOR THE QUANTITATIVE DETERMINATION OF THE SOLID SUPPORTED FUNCTIONAL GROUPS ON THE SURFACE OF VARIOUS COMMERCIALLY AVAILABLE SOLID CARRIERS (PLASTIC BEADS, POLYSACCHARIC MICROPARTICLES, MICROTITRATION PLATES) USED IN IMMUNOASSAYS, AFFINITY CHROMATOGRAPHY TECHNIQUES, NUCLEIC ACID HYBRIDIZATION TECHNIQUES ETC.

Topics: BICINCHONINIC ACID (BCA), COLORIMETRIC DETERMINATION, COVALENT IMMOBILIZATION, REACTIVE SOLID SUPPORTED FUNCTIONAL GROUPS, SOLID CARRIERS, SOLID SUPPORTS, TOTAL SOLID SUPPORTED FUNCTIONAL GROUPS, ΔΙΚΙΓΧΟΝΙΝΙΚΟ ΟΞΥ, ΔΡΑΣΤΙΚΕΣ ΟΜΑΔΕΣ ΣΤΕΡΕΩΝ ΦΟΡΕΩΝ, ΟΛΙΚΕΣ ΟΜΑΔΕΣ ΣΤΕΡΕΩΝ ΦΟΡΕΩΝ, ΟΜΟΙΟΠΟΛΙΚΗ ΣΥΝΔΕΣΗ, ΣΤΕΡΕΟΙ ΦΟΡΕΙΣ, ΦΑΣΜΑΤΟΦΩΤΟΜΕΤΡΙΚΟΣ ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΟΜΑΔΩΝ ΣΤΕΡΕΩΝ ΦΟΡΕΩΝ
Publisher: University of Patras
Year: 1994
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