Drug delivery

Abstract

Hydroxyapatite (HA) is the principal constituent of bone mineral, and synthetic HA is widely used as enous grafts including the restricted amount of donor bone available but allograft has diminished osteoinductivity due to processing steps that eliminate cells and also denature or destroy osteoregenerative HA, and HA-containing composite tissue engineering scaffolds. by which native gic HA present in bone sialoprotein omains consisting of contiguous aspartate (D) or glutamate (E) residues that interact with the calcium within HA [4e6]. Prior studies from our group and others have shown that polyaspartate or polyglutamate do-mains can be used to anchor multiple bioactive peptides onto HA including the integrin-binding peptide, RGD [7e9], the proteoglycan-binding peptides, FHRRIKA and KRSR [10], and an osteoinductive collagen-derived peptide, DGEA [11]. For example, the addition of a heptaglutamate domain (E7) to the DGEA peptide markedly increased the amount of peptide loadedonto syntheticH