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The influence of defined ante-mortem stressors on the early post-mortem biochemical processes in the abdominal muscle of the Norway lobster, Nephrops norvegicus (Linnaeus, 1758)

By S. Gornik, A. Albalat, R. Atkinson, G. Coombs and D. Neil

Abstract

The effects of four different ante-mortem stressors (exercise, emersion, starvation and a patent infection with the parasite Hematodinium sp.) on post-mortem processes have been investigated in the abdominal muscle of Norway lobster Nephrops norvegicus by measuring changes in the pH, the levels of glycogen, l-lactate, arginine phosphate, ATP, ADP, AMP, IMP, HxR, Hx and the adenylate energy charge (AEC) over a time course of 24 h with samples being taken at 0, 3, 6, 12 and 24 h. The acute stresses of intense exercise and 2 h emersion resulted in a premature onset of anaerobic glycolysis, leading both to an enhanced glycogen depletion rate and an early accumulation of l-lactate. The chronic stressors, starvation and parasite infection, resulted in a complete ante-mortem depletion of muscle glycogen and consequently the failure of post-mortem glycolytic fermentation. Post-mortem pH and ATP inter-conversion were significantly altered in chronically stressed animals. Ante-mortem, a rapid, almost complete depletion of arginine phosphate was observed in all stress groups. The AEC was altered significantly by all stresses, indicating a strong energy demand. The findings suggest that ante-mortem stressors strongly influence the post-mortem biochemical processes. The laboratory-based results are compared to 'field' data and effects on post-harvest product quality are discussed

Publisher: Taylor and Francis
Year: 2010
OAI identifier: oai:eprints.gla.ac.uk:45016
Provided by: Enlighten

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  1. (2005). A review of the parasitic dinoflagellates Hematodinium species and Hematodinium-like infections in marine crustaceans.
  2. (1979). Activation of AMP aminohydrolase during skeletal-muscle contraction.
  3. (2005). AMPdeaminase in elasmobranch fish: A comparative histochemical and enzymatic study.
  4. (2009). Biochemical investigations into the absence of rigor mortis in Norway lobster Nephrops norvegicus.
  5. (1965). Biological feedback control at the molecular level.
  6. Catch me in winter! Seasonal variation in air temperature severely enhances physiological stress and mortality of species subjected to sorting operations and discarded during annual fishing activities.
  7. (1977). Cellular energy metabolism and its regulation.
  8. (2001). Changes in baseline levels of nucleotides during ice storage of fish and crustaceans from the Portuguese coast.
  9. (1982). Chemical and sensory changes in iced Nephrops norvegicus as indices of spoilage.
  10. (1995). Chemistry in the kitchen: the chemistry of flesh foods I.
  11. (1969). Comparative aspects of muscle.
  12. (2001). Crustacean Hyperglycemic Hormone family: old paradigms and new perspectives.
  13. (1985). D-Glucose: Determination with hexokinase and glucose-6-phophate dehydrogenase. In: Bergmeyer HU,
  14. (1985). Determination of adenosine triphosphate and its breadown products in fish muscle by high-performance liquid chromatography.
  15. (2001). Development and application of an immunoassay diagnostic technique for studying Hematodinium infections in Nephrops norvegicus populations.
  16. (2007). Difference in tenderness and pH decline between water buffalo meat and beef during post-mortem ageing.
  17. (1992). Ecape Swimming in the Norway Lobster.
  18. (1990). Effect of aerial exposure on concentrations of selected metabolites in the blood of the Norway lobster Nephrops norvegicus (Crustacea:
  19. (1976). Effect of ante-mortem environmental temperatures on post-mortem glycolysis and tenderness in excised broiler breast muscle.
  20. (2009). Effect of capture method on the physiology and nucleotide breakdown products in the Norway lobster (Nephrops norvegicus).
  21. (1999). Effect of some ante-mortem stressors on peri-mortem and post-mortem biochemical changes and tenderness in broiler breast muscle: a review. World Poult Sci
  22. (1997). Effects of ambient ammonia levels on blood ammonia, ammonia excretion and heart and scaphognathite rates of Nephrops norvegicus. doi
  23. (1984). Effects of oxygen deprivation during anoxia and muscular work on the energy metabolism of the crayfish Orconectes limosus.
  24. (1986). Energetics of hermit crabs during locomotion: the cost of carrying a shell.
  25. (1993). Flesh quality in snapper Pagrus auratus affected by capture stress.
  26. (1966). Glycolytic metabolites and their distribution at death in the white and red muscle of cod following various degrees of antemortem muscular activity.
  27. (1997). Handling stress and storage temperature affect meat quality of farmed-raised Atlantic salmon (Salmo salar).
  28. (1986). IMP response: an indicator of metabolic stress in working muscle.
  29. (2006). Impact of capture method and trawl duration on the health status of the Norway lobster Nephrops norvegicus.
  30. (2007). Improvement of the commercial quality of chilled Norway lobster (Nephrops norvegicus) stored in slurry ice: effects of a preliminary treatment with an antimelanosic agent on enzymatic browning. Food Chem 103: 741-748
  31. (1987). In situ measurements of adenylate energy charge and assessment of pollution.
  32. (1965). Influence of ante-mortem stress on meat palatability.
  33. (2000). Initial changes in ATP equivalents in the post-mortem muscles of three species of lobster. doi
  34. (1981). Interaction between ante-mortem stress sampling time and pH found in certain pork muscle characteristics.
  35. (2005). Invertebrate muscles: muscle specific genes and proteins
  36. (1999). L-lactate metabolism and glucose turnover in the subterranean crustacean Niphargus virei during post-hypoxia recovery.
  37. (1985). Lactate dehydrogenase. In:
  38. (2007). Mechanisms controlling pork quality development: the biochemistry controlling post-mortem energy metabolism. doi
  39. (1986). Metabolic adaptation of the horseshoe crab, Limulus polyphemus, during exercise and environmental hypoxia and subsequent recovery.
  40. (2001). Metabolic characteristics of muscles in the Spiny lobster Jasus edwardsii and responses to emersion during simulated live transport.
  41. (2007). Metabolic responses and arginine kinase expression under hypoxic stress of the kuruma prawn Marsupenaeus japonicus.
  42. (1987). Metabolic responses of the prawns Palaemon elegans and P. serratus (Crustacean: Decapoda) to acute hypoxia and anoxia.
  43. (2006). Morbidity and mortality in Norway lobsters Nephrops norvegicus: physiological immunological and pathological effects of aerial exposure.
  44. (1980). Moulting and growth. In:
  45. (1993). Myofibrillar protein composition correlates with histochemistry in fibres of the abdominal flexor muscle of the Norway lobster Nephrops norvegicus.
  46. (1987). Nutritional status and energy metabolism of crayfish (Procambrus clarkii Girard) muscle and hepatopancreas. doi
  47. (2001). Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography.
  48. (1991). Physiological and metabolic responses of the shore crab Carcinus maenas (L) during environmental anoxia and subsequent recovery.
  49. (2005). Physiological changes in the Norway lobster Nephrops norvegicus (L) escaping and discarded from commercial trawls on the West Coast of Scotland: I Body fluid volumes and haemolymph composition after capture and during recovery.
  50. (1974). Post-mortem changes and spoilage in Rock lobster muscle.
  51. (2006). Pre-slaughter transport AMP-activated protein kinase glycolysis and quality of pork loin. doi
  52. (1995). Quality and quality changes in fresh fish,
  53. (1995). Regulation of skeletalmuscle AMP deaminase: Involvement of histidine residues in the pH-dependent inhibition of the rabbit enzyme by ATP.
  54. (1988). Respiration, ventilation and circulation under hypoxia in the glacial relict Saduria (Mesidotea) entomon.
  55. (1990). Seafood: Resources Nutritional Composition and Preservation Sikorski Z E (ed) Chapter 4 - Postharvest biochemical and microbial changes. Boca
  56. (1996). Spoilage pattern of five species of Australian prawns: deterioration is influenced by environment of capture and mode of storage.
  57. (1980). Starvation effect on metabolism of Penaeus japonicus.
  58. (1988). Swimming performance and endurance of the Norway lobster Nephrops norvegicus.
  59. (1975). The contents of adenine nucleotides phosphagens and some glycolytic intermediates in resting muscle from vertebrates and invertebrates.
  60. (1979). The post-mortem pH of cod and haddock muscle and its seasonal variation.
  61. (2002). The prevalence of Hematodinium in Nephrops norvegicus from the western.
  62. (1983). The recovery from anaerobic metabolism in invertebrates.
  63. (2001). The relationship of Hematodinium infection prevalence in a Scottish Nephrops norvegicus population to season moulting and sex.
  64. (1993). The rise in inosine monophosphate and L-L-lactate concentrations in muscle of live panaeid prawns (Penaeus japonicus Penaeus monodon) stressed by storage out of water.
  65. (2008). The time course of early post-mortem biochemical processes in the abdominal muscle of a commercially important decapod crustacean (Nephrops norvegicus): implications for post-catch processing.
  66. (2006). Trends in postmortem aging in fish: understanding of proteolysis and disorganization of myofibrilliar structure.
  67. (2006). Usage of energy reserves in crustaceans during starvation: status and future directions.
  68. (1999). Use of the rigor mortis process as a tool for better understanding of skeletal muscle physiology: effect of the ante-mortem stress on the progression of rigor mortis in brook charr (salvelinus fontinalis).
  69. (2002). Utilisation of glycogen ATP and arginine phosphate in exercise and recovery in terrestrial red crabs Gecarcoidea natalis.

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