Isolation and characterization of a Drosophila hydei Histone DNA repeat unit

Abstract

Histone genes in D. hydei are organized in tandemly repeated clusters., accomodating in total 120- 140 repeat units. We cloned one of the repeat units and analysed the nucleotide sequence. The repeat unit has a size of 5.1 x lo3 base-pairs and contalns one copy of each of the genes coding for the core histones and one copy d i n g for the histone HI. In the promoter regions of the genes we identifled the presumptive cap sites and TATA boxes. Two additional sequence elements are shared by all five Dmophila hydel histone genes in the cluster. The sequence CCCTCT/G1 is found in the region upstream of the presumptive CAP sites. The sequence element AGTGAA occurs downstream of the presumptive cap sites and is, in contrast to the promoter element, also seen in the hlstone genes of Drosophila melanogaster [I]. Cellcycle dependent regulation of transcription of the Dmophila histone genes may be different from that in other eukaryotes since sequence elements involved in the regulation of cellcycle dependent transcription are absent. Also other regulatory elements for transcription differ from those of other genes. The highly consewed HI-specific promoter sequence AAACACA and the H2B specific promoter sequence ATTTGCAT, which are involved in the cell-cycle dependent transcription of those histone genes in eukaryotes, are missing in the Drosophlla genes. However at the 3 ' end of the genes the palindrome and the purine-rich reglon, both consewed sequence elements in histone genes of eukaryotes, are present. The spacer regions show a simple sequence organization. The silent site substitution rate between the coding regions of the D. hydel and D. melanogester histone genes is at least 1.5 times higher for Dnrsophlla than for sea urchin histone genes