Genotyping of Toxoplasma gondii isolates from wild boars in peninsular Malaysia

Abstract

Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of the world population. The present study was done to isolate and genotype T. gondii from wild boar from forests of Pahang, Malaysia. A total of 30 wild boars ’ blood, heads and hearts were obtained for this study and 30 (100.0%) were found to be seropositive when assayed with modified agglutination test (MAT$6). The positive samples were inoculated into mice and T. gondii was only isolated from samples that had strong seropositivity (MAT$1:24).The isolates were subjected to PCR-RFLP analysis and all the Peninsular Malaysia isolates of T. gondii are of clonal type I