Graduation date: 1991In vivo research following ingesting of commercially available\ud Lactobacillus tablets, which contain about 2X10⁶ cfu/tablet of\ud Lactobacillus acidophilus and Lactobacillus bulgaricus cells in a dose\ud of four tablets daily, showed serum lipoprotein concentrations did not\ud change significantly. In order to increase the number of viable\ud Lactobacillus bacteria after challenging in low pH solution (gastric\ud fluid), enteric coating polymer was applied over dried calcium alginate\ud beads containing Lactobacillus. Survival of Lactobacillus bacteria was\ud generally higher from freeze dried calcium alginate beads compared to\ud vacuum dried products. However, after pretreatment with simulated\ud gastric fluid (pH = 1.5) for 2 hours, only the coated products from\ud vacuum drying showed promising results. Lactobacillus bacteria were\ud fully protected against gastric pH after formulating the bacteria inside\ud mini-tablets which were coated with Eudragit L30D, an enteric coating\ud polymer.\ud Alginic acids are naturally occurring substances found only in the\ud brown seaweeds. Alginic acid salts formed with most di-, and polyvalent\ud metals are insoluble in water. The most common application of alginate\ud precipitation in drug product formulation is based on insolubilization\ud of alginate by addition of calcium salt. By altering the composition of\ud calcium alginate, drug loading, enteric coating thickness, and sustained\ud release coating thickness, the lag time for drug dissolution can be\ud controlled. This formulation research provides oral dosage form design\ud for targeted delivery of drug to any desired site in the\ud gastrointestinal tract. Examples of site specific targeted delivery are\ud given for Lactobacillus bacteria, ibuprofen, sulfasalazine, and\ud 5-aminosalicylic acid
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