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Molecular factors that influence host range and virulence of A. tumefaciens



Graduation date: 1991Agrobacterium tumefaciens is a common soil bacterium that causes crown gall\ud disease on many dicotyledonous plant species. It has been exploited for use in genetic\ud engineering because it is able to promote transfer of any DNA segment to plant\ud chromosomes. Many molecular factors in both the plant and bacterium contribute to\ud successful infection and DNA transfer.\ud Two bacterial factors were studied for this dissertation. The first was bacterial\ud synthesis of cytokinins due to the induced expression of the gene tzs. Cytokinin\ud production by some A. tumefaciens strains at the time of infection was hypothesized to\ud enhance infection or influence host range. To test this hypothesis, heterologous strains\ud engineered to express tzs and a native strain from which tzs was deleted were evaluated\ud for tumor induction on a number of plant species. Major differences were seen when\ud radish roots were infected with the tzs deletion strain. Morphological differences of the\ud galls formed suggested a possible role for tzs in T-DNA transfer efficiency. β-glucuronidase\ud assays with a mannopine synthase promoter-uidA fusion gene were\ud used to study T-DNA transfer early in the infection process. No biological role for tzs\ud was confirmed with either tumorigenesis or T-DNA transfer assays. However, optimized\ud conditions for GUS fluorometric assays were established and the infection process was\ud characterized.\ud The second factor studied was a β-glucosidase gene in A. tumefaciens strain\ud 83/73. This strain is highly infective on conifer hosts, whereas others are not. It is also\ud able to hydrolyze the conifer phenolic compound, coniferin, to an active virulence gene\ud inducer, coniferyl alcohol. This appears to be a major host range determining factor.\ud As a first step in proving this role, the gene for the β-glucosidase enzyme responsible\ud for hydrolysis of coniferin in strain B3/73 was cloned and sequenced. The deduced\ud protein sequence has homology to other β-glucosidases, including a conserved active\ud site

Year: 1990
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Provided by: ScholarsArchive@OSU

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  1. (1986). 13- Glucuronidase from Escherichia coli as a gene-fusion marker.
  2. (1983). A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid.
  3. (1983). A chimeric antibiotic resistancegene as a selectable marker for plant cell transformation.
  4. (1990). A chromosomal Agrobacterium tumefaciensgene required for effective plant signal transduction.
  5. (1984). A comprehensive set ofsequence analysis programs for the VAX.
  6. (1986). A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
  7. (1970). A Handbook of Systematic Botany. Asia PublishingHouse,
  8. (1986). A plant cell factor induces Agrobacterium tumefaciens vir gene expression.
  9. (1986). A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes.
  10. (1987). A plasmid sequence from Rhizobium leguminosarum 300 contains homology to sequences near the octopine TL-DNA right border.
  11. (1979). A rapid alkaline extractionprocedure for screening recombinant plasmid DNA.
  12. (1986). A rapid method for assaying tumorigenicity of Agrobacterium tumefaciens.
  13. (1990). A regulatorygene as a novel visible marker for maize transformation.
  14. (1982). A simple method for displaying the hydrpathic character of a protein.
  15. (1983). A technique for radiolebeling DNA restriction endonuclease fragments to high specific activity.
  16. (1986). Adenovirus replication in trans; New replication pathway.Doctoral thesis,
  17. (1987). Agrobacterium mediated transformation and regeneration of Populus.
  18. (1982). Agrobacterium Ti plasmid indoleacetic acid gene is required for crown gall oncogenesis.
  19. (1980). Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.
  20. (1982). Agrobacterium tumefaciensmutants affected in attatchment to plant cells.
  21. (1990). Agrobacteriummediated transformation of rice (Oryza sativa L.).
  22. (1986). Agroinfection",an alternative route for viral infection of plants by using the Ti plasmid.
  23. (1985). An erythromycin-resistance gene from an erythromycin-producing strain of Arthrobacter sp.
  24. (1986). Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.
  25. (1989). Analysis of the major inducers of the Rhizobium nodA promoter from Vicia sativaroot exudate and their activity with different nodD genes. Plant Mol.
  26. (1985). Analysis of transfer of tumor-inducingplasmids from Agrobacterium tumefaciens to Petunia portoplasts.
  27. (1988). Anatomy of tumor development in grape stem tissue inoculated with Agrobacterium tumefaciens biovar 3,
  28. (1988). and E.W.Nester
  29. (1987). and P.Zambryski
  30. (1986). and R.B.Horsch
  31. (1987). Assaying chimeric genes in plants: the GUSgene fusion system.
  32. (1988). Basic Processis underlying Agrobacterium-mediated DNA transfer to plant cells.
  33. (1974). Biostatistical Analysis. Prentice-Hall,
  34. (1986). Both TATA box andupstream regions are required for the nopaline synthase promoter activity in transformed tobacco cells.
  35. (1985). Brassica grown gall tumorigenesis and in vitro of transformed tissue.
  36. (1980). Broad hostrange DNA cloning system for Gram-negative bacteria: Construction ofa gene bank of Rhizobium meliloti.
  37. (1988). Cell biology ofAgrobacterium infection and transformation of plants.
  38. (1989). Cell type expression of 35S CaMV-GUS reporter gene in transgenic soybean plants.
  39. (1987). Characterization of nonattaching mutants of Agrobacterium tumefaciens.
  40. (1987). Characterization of the virA locus of Agrobacterium tumefaciens: A transcriptional regulator and host range determinant.
  41. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
  42. (1986). Clones form a shooty tobacco crown gall tumor II: Irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes.
  43. (1984). Cloning and expression of the structural gene for B-glucosidase of Kluyveromyces fragilis in Escherichia coil and Saccharomyces cerevisiae.
  44. (1985). Cloning and nucleotidesequence of the tzs gene from Agrobacterium tumefaciens strain T37.
  45. (1985). cloning vehicles for transformation of higher plants.
  46. (1986). Cloningand expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acidbiosynthesis genes in Escherichia coli.J.
  47. (1987). Cloverssecrete specific phenolic compounds which either stimulateor repress nod genes expression in Rhizobium trifolii.
  48. (1983). Compilation and analysis of Escherichia coli promoter sequences.
  49. (1952). Conditioning of the host cellas a factor in the transformation process in crown gall.
  50. (1987). Conserved domains in bacterial regulatory protiens that respond to environmental stimuli.
  51. Constantino(1985) Identification of the genetic locus responsible for non-polar root induction by Agrobacterium rhizogenes 1855. Plant Mol.
  52. (1989). Cooperative interaction of Agrobacterium virE2 protein with single-stranded DNA: implications for the T-DNA transfer process.
  53. (1986). Covalent modification of the gInG product, NRI, by glnL product, NRii, regulates the transcription of the glnALG operon in Escherichia coli.
  54. (1987). Cytokinin production by Agrobacterium and Pseudomonas spp.
  55. (1989). Cytokinin production by Bradyrhizobium japonicum.Plant
  56. (1983). Cytokinin/auxin balance incrown gall tumors is regulated by specific loci in the T-DNA.
  57. (1978). Cytokinins. In Phytohormones and Related Compounds-
  58. (1987). Deletion analysis of the mannopinesynthase gene promoter in sunflower crown gall tumors and Agrobacterium tumefaciens.
  59. (1986). Detection of T-DNA transfer to plant cells by A. tumefaciens virulence mutants using agroinfection.
  60. (1967). Development in Flowering Plants.
  61. (1986). Development of plant promoter expressionvectors and their use for analysis of differential activity of nopaline synthase promoter in transformedtobacco cells.
  62. (1986). Effects of cytokinins on fungus development and host response in powdery mildew of barley.
  63. (1989). Efficacy of different Agrobacterium tumefaciens strains in transformation of pinaceous gymnosperms.
  64. (1990). Efficient transformation of Pinaceous gymnosperm cells by Agrobacterium. Doctoral thesis,
  65. (1981). Elaboration of cellulose fibrils by Agrobacterium tumefaciens during attachment to carrot cells.
  66. (1983). Evidence for use ofrare codons in the dnaG gene and other regulatory genes of Escherichia coli.
  67. (1987). Evidence that DNA involved in the expression of nodulation (nod) genes in Rhizobium binds to the product of the regulatory gene nodD.
  68. (1989). Expression of Agrobacteriumnopaline-specific virD1, virD2, and virC1 proteins and their requirement for T-strand productionin E.
  69. (1988). Expression of an Agrobacterium Ti plasmidgene involved in cytokinin biosynthesis is regulated by virulence loci and induced by plant phenolic compounds.
  70. (1983). Expression of bacterial genes in plant cells.
  71. (1988). Factors influencing gene delivery intoZea mayscells by high-velocity microprojectiles.
  72. (1990). Feasibility studies using B-glucuronidase as a gene fusion marker
  73. (1978). Fingerprints of Agrobacterium Ti plasmids.
  74. (1986). Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants.
  75. (1989). Flavonoidcompounds from pollen and stigma of Petunia hybrida: inducers of the vir region ofthe Agrobacterium tumefaciens Ti plasmid.
  76. (1987). Four classes of mutations in the nodD gene of Rhizobium leguminosarum biovar. viciae that affect its ability to autoregulate and /or activate other nod genes in the presence of flavanoid inducers.
  77. (1986). Generation of singlestranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells.
  78. (1987). Genes responsible for the supervirulence phenotype ofAgrobacterium tumefaciensA281.
  79. (1981). Genetic analysis of crown gall: Fine structuremap of the T-DNA by site directed mutagenesis.
  80. (1982). Genetic factors controlling the host rangeAgrobacterium tumefaciens.Phytopath.
  81. (1982). Genetic identification of functions of TL-DNA transcripts in octopine crown galls.
  82. (1987). GUS Fusions: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
  83. (1985). High efficiency transformation of cultured tobacco cells.Plant
  84. (1989). High levels of double-stranded transferred DNA (T-DNA) processing from an intact nopaline Ti plasmid.
  85. (1979). Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens.
  86. (1984). Host range encoded by the Agrobacterium tumefaciens tumor-inducing plasmid pTiAg63 can be expanded by modification of its T-DNA oncogene complement.
  87. (1980). Host range of Agrobacterium tumefaciens is determined by the Ti plasmid.
  88. (1979). Host specificity in thegenus Agrobacterium.
  89. (1987). Host-specific regulation of nodulation genes in Rhizobium is mediated by a plant-signal, interacting with the nodD gene product.
  90. (1987). Identification of a new virulence locus in Agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment.
  91. (1990). Identification of an Agrobacterium tumefaciens virulence gene inducer from the pinaceous gymnosperm Pseudostuga menziesii.
  92. (1985). Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens.
  93. (1987). Identification ofan essential upstream element in the nopaline synthase promoter by stable and transientassays.
  94. (1985). Identificationand genetic analysis of an Agrobacterium tumefaciens chromosomal virulenceregion.
  95. (1986). Identifyingnonpolar transbilayer helices in amino acid sequences of membrane proteins.
  96. (1982). Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens.
  97. (1981). Induction of tumors on Solanum tuberosum L. by Agrobacterium: Quantitative analysis, inhibition by carbohydrates, and virulence of selected strains.
  98. (1988). Induction ofa nodA-lacZ fusion in Bradyrhizobium japonicum by an isoflavone.
  99. (1986). Initial interactions of Agrobacterium tumefaciens with plant host cells.
  100. initiates translation at a UUG codon and is a sequence-specific DNA-binding protein.
  101. (1980). Integration and organization of Ti plasmid sequences in crown gall tumors.
  102. (1985). Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciensto plant cells.
  103. (1980). Isolation and structure ofa tryptic glycopeptide from the active site of B-glucosidase A3 from Aspergillus wentii.
  104. (1984). Isolation of adual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens.
  105. (1985). Limited-host-range plasmid of Agrobacterium tumefaciens: Molecular and genetic analyses of transferred DNA.
  106. (1980). Localization of the replication control region on the physical map of the octopine Ti plasmid.
  107. (1988). Mechanism of activationof Rhizobium nodD gene.
  108. (1989). Membrane topology and functional analysis of the sensory portein virA of Agrobacterium tumefaciens.
  109. (1978). Modificationof disease resistance of tobacco callus tissues by cytokinins. Plant Physiol.
  110. (1985). Molecular and genetic analysis of factors controling host range in Agrobacterium tumefaciens.
  111. (1986). Molecular basis for the auxin-independent phenotype of crown gall tumor tissues.
  112. (1986). Molecular characterization of a host-rangedetermining locus from Agrobacterium tumefaciens.
  113. (1986). molecular cloning and expression of a cellobiase gene from an Agrobacteriumin Escherichia coli.
  114. (1980). Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58as a means to restriction endonuclease mapping.
  115. (1982). Molecular Cloning.
  116. (1985). Multiple mechanisms of protein insertioninto and across membranes.
  117. (1983). Multiple mutation in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid.
  118. (1983). Mutational analysis of the virulence region of anAgrobacterium tumefaciensTi plasmid.
  119. Nester(1975) Plasmid required for virulence of Agrobacterium tumefaciens.
  120. Nester(1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes.
  121. (1989). Nucleotide sequence and analysis of the plantinducible locuspinFfromAgrobacterium tumefaciens.J.
  122. (1986). Nucleotide sequence and expression ofa Pseudomonas savastanoi cytokinin biosynthetic gene: Homology with Agrobacterium tumefaciens tmr and tzs loci.
  123. (1989). Nucleotide sequence of the tzs gene from Agrobacterium rhizogenes strain A4.
  124. (1987). Nucleotide sequence of the virG locus of the Agrobacterium tumefaciens plasmid pTiC58.
  125. (1985). Nucleotide sequence ofCandida pelliculosaBglucosidase gene.
  126. (1988). Nucleotide sequences of Saccharomycopsis fibuligera genes for extracellular B-glucosidases as expressed in Saccaromyces cerevisiae.
  127. (1985). Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaciens T-DNA.
  128. (1983). Nucleotidesequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmidpTi15955. Plant Mol.
  129. (1989). Nucleotidesequence of the tzs gene from Pseudomonas solanacearum strain K60. Nuc. Acids Res.
  130. (1982). Occurence and biosynthesis of opines. In Molecular Biology of Plant Tumors.
  131. (1982). Octopine accumulation early in crown gall development is progressive.
  132. (1977). Octopine and nopaline metabolism in Agrobacterium tumefaciens andcrown gall tumor cells: role of plasmid genes.
  133. (1976). Octopine and nopaline synthesis and breakdowngenetically controlled by a plasmid of Agrobacterium tumefaciens.
  134. (1984). of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.
  135. (1987). of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear,singlestranded T-DNA.
  136. (1986). Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid.
  137. (1966). Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines.
  138. (1986). Plant cells induce transcription of theAgrobacterium tumefaciensnopaline pTiC58 virulence region.
  139. (1987). Plant Galls and Gall Inducers. Gebruder Borntraeger,
  140. (1988). Plant genetic engineering: Progress and promise.
  141. (1986). Plant phenoliccompounds induce expression of the Agrobacterium tumefaciens loci needed for virulence.Science 232:
  142. (1989). Promoters of the rolA, B and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plants.
  143. (1969). Purification and characterization of B-glucosidase of Alcaligenes faecalis.
  144. (1986). Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.
  145. (1981). Rapid procedure for detection and isolation of large and small plasmids.
  146. (1989). Recoveryof Agrobacterium tumefaciens T-DNA molecules from whole plants early aftertransfer.
  147. (1980). Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance.
  148. (1987). Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.
  149. (1980). Relationships between cytokinin production, presence of plasmids, and fasciation caused by strains of Corynebacterium fascians.
  150. (1984). Restoration of virulence of vir region mutants of Agrobacterium tumefaciens strain B6S3 by coinfection with normal and mutant Agrobacterium strains.
  151. (1984). Restriction endonuclease map of pTiBo542, a potential Ti plasmid vector for genetic engineering of plants.
  152. (1988). Rhizobium meliloti nodA and nodB genes are involved in generating compounds that stimulate mitosis of plant cells.
  153. (1989). Rhizobium-legume nodulation: life together in the underground.Cell
  154. (1989). Role of Agrobacterium tumefaciens chvA protein in export of beta-1,2-glucan.J.
  155. (1983). Role of bacterial cellulose fibrils in Agrobacterium tumefaciens infection.
  156. (1982). Secretion of trans-zeation by Agrobacterium tumefaciens: A function determined by the nopaline Ti plasmid.
  157. (1970). Selective media for isolation of Agrobacterium,
  158. (1987). Sequence and transcription of the B-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae.
  159. (1988). Sequence structure and expressionof a cloned B-glucosidase gene from an extreme thermophile.
  160. (1988). Specificity of signal compounds detected by Agrobacterium tumefaciens.
  161. (1989). Specificity of signal molecules in the activation of Agrobacterium virulence gene expression.
  162. Stachel (1987)Characterization of Agrobacterium tumefaciens virulence proteins induced bythe plant factor acetosyringone.
  163. (1987). Strain and cultivar specificity in the Agrobacterium-soybean interaction.
  164. (1988). Structure and transcription analysis of the gene encoding a cellobiasefrom Agrobacterium sp. strain ATCC 21400.
  165. (1977). Studies on Agrobacterium tumefaciens. VII. Avirulence induced by temperature and ethidium bromide.
  166. (1984). Succinamopine:a new crown gall opine.
  167. (1974). Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.
  168. (1972). Suppression of the bacterially induced hypersensitive reaction by cytokinins.
  169. (1990). Symbiotic host-specificity of Rhizobium meliloti is determined bya sulphated and acetylated glucosamine oligosaccharide signal.
  170. (1987). T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives.
  171. (1978). Taxonomy and host specificity of thegenus Agrobacterium.
  172. (1988). Temperature-sensitive step in Ti plasmid vir-region induction and correlation with cytokinin secretion by Agrobacteria.
  173. (1974). The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites.
  174. (1988). The Agrobacterium tumefaciens T-DNA gene 6u is an one gene.
  175. (1989). The Agrobacterium tumefaciens yirCl gene product binds to overdrive, a T-DNA transfer enhanser.
  176. (1989). The CaMV 35Senhancer contains at least two domains which can confer different developmental and tissue-specificexpression patterns.
  177. (1981). The classification, identification and nomenclature of agrobacteria.
  178. (1985). The crucifer genetics cooperative. Plant Mol.
  179. (1968). The cytokinins.
  180. (1986). The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens.
  181. (1976). The host range ofcrown gall.
  182. (1986). The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside the TDNA.
  183. (1988). The kinetics of T-strandproduction in a nopaline-type helper strain of Agrobacterium tumefaciens.
  184. (1984). The limited host range of an Agrobacterium tumefaciens strain extended bya cytokinin gene from a wide host range T-region.
  185. (1987). The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants.
  186. (1990). The regulatory virG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation ofAgrobacterium tumefaciensvirulence genes.
  187. (1986). The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate.
  188. (1986). The right border region of pTiT37 T-DNA is intrinsically more active than the left border region in promoting T-DNA transformation.
  189. (1986). The transformation of Zeamays seedlings with Agrobacterium tumefaciens. Plant Mol.
  190. (1986). The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease.
  191. (1989). The virD operon of Agrobacterium tumefaciens Tiplasmid encodes a DNA-relaxing enzyme.
  192. (1989). ThenodH and nodQ host range genes of Rhizobium meliloti behave as avirulence genes in R. leguminosarum by. viciae and determines changes in the production of plant-specificextracellular signals.
  193. (1990). ThevirA protein ofAgrobacterium tumefaciensis autophosphorylated and is essential forvir gene regulation.
  194. Thomashow (1984a) Comparison of T-DNAoncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges.
  195. (1988). Three regulatory nodD allelesof diverged flavanoid-specificity are involved in host-dependent nodulation by Rhizobium meliloti.
  196. (1989). Ti plasmid containing Rhizobium meliloti are non-tumorigenic onplants, despite proper virulence gene induction and T-strand formation.
  197. (1986). Transcription termination and the regulation ofgene expression.
  198. (1990). Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation and acidic growth media.
  199. (1989). Transferand function of T-DNA genes from Agrobactium Ti and Ri plasmds in plants.
  200. (1989). Transformation of Brassicanapus and Brassica oleracea using Agrobacterium tumefaciens and the expression ofthe bar and neo genes in the transgenic plants. Plant Physiol.
  201. (1987). Transformation of Brassicanapus with Agrobacterium tumefaciens based vectors. Plant Cell Rep.
  202. (1987). Transgenic plants of Brassica napus L.
  203. (1986). Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.
  204. (1989). Transposable elements can be used to study cell lineages in transgenic plants
  205. (1962). Tumor inception and development in thecrown gall disease.
  206. (1987). Two Agrobacterium tumefaciens genes for cytokinin biosynthesis: Ti plasmid-coded isopentenyltransferase adapted for function in prokaryotic or eukaryotic cells.
  207. (1988). Two host-inducible genes of Rhizobium fredii and characterization of the inducing compound.
  208. (1986). Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.
  209. (1986). Tzs, a nopaline Ti plasmidgene from Agrobacterium tumefaciens associated with trans-zeatin biosynthesis.
  210. (1988). Ultrastructural observations of the process of Agrobacterium tumefaciens biovar 3 infection of grape cv.
  211. (1989). Use of a root tumorigenesis assay to detect genotypic variation in susceptibility of thirty-four cultivars of Pisum sativum to crown gall.
  212. (1985). Use of TR T-DNA promoterto express genes in plants and bacteria.
  213. (1988). Vir box sequences
  214. (1986). VirA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens.
  215. (1988). VirD2 protein of Agrobacteriumtumefaciens very tightly bound at the 5' end of the T-strand DNA.
  216. (1985). Virulence properties of strains of Agrobacterium tumefaciens on the apical and basal surfaces of carrot root discs.
  217. Zambryski(1984) Right 25 by terminus sequence of the nopaline T-DNA is essential for and determinesdirection of DNA transfer from Agrobacterium to the plant genome.
  218. Zambryski(1987) Sequence context of the T-DNA border repeat element determines itsrelative activity during T-DNA transfer to plant cells.

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