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An anaerobic microcosm study of TCE transformation by microbes stimulated from the Gilbert-Mosley site, Wichita, Kansas

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Graduation date: 1996A mix-culture of methanogenic and sulfate-reducing bacteria was capable of degrading trichloroethylene (TCE) to dichloroethylene (DCE). The culture was incubated under anaerobic conditions within a soil sample taken from Gilbert-Mosley site, Wichita, Kansas. To stimulate the growth of indigenous bacteria, a carbon and energy source was added in this microcosm study without the addition of nutrients. Each microcosm contained various amounts of groundwater and sediment. Benzoate was the primary carbon and energy source added to the various modified microcosms. Several other carbon and energy sources were also tested including: methanol, ethanol, acetate, propionate, butyrate, toluene and phenol. Except for toluene and phenol, the other substrates were able to enhance the degradation of TCE under sulfate-reducing conditions. The microcosm control to which no substrate was added showed no signs of TCE transformation. Addition of suitable carbon and energy sources did enhance the sulfate-reducing conditions and TCE transformation in this microcosm study.\ud No TCE transformation was observed under denitrifying conditions, although the\ud groundwater contained about 46 mg/L of nitrate. Nitrate reduction appeared earlier and the rate was faster than that of sulfate reduction. Addition of nitrate did enhance denitrifying conditions which are co-existent with sulfate reducing conditions.\ud Under sulfate reducing conditions, where the sulfate concentration was reduced to less than 150 mg/L, rapid degradation of TCE occurred within a time interval of 30 days. The transformation of TCE in some case followed a lag after sulfate reduction. The reduction mechanism of TCE transformation remained unknown. However, TCE transformation usually coincided with sulfate reduction. Some methane production was also observed at low sulfate concentrations. Under sulfate-reducing conditions, 1,2 c-DCE was the major end product and it persisted over 300 days without further degradation. It is consistent with the field observation. Some minor traces of ethylene were found, and accumulation of vinyl chloride was not observed

Year: 1995
OAI identifier: oai:ir.library.oregonstate.edu:1957/34776
Provided by: ScholarsArchive@OSU

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Citations

  1. (1986). A Extended Abstract,
  2. (1990). A Field Evaluation of In-situ Biodegradation of Chlorinated Ethenes: Part 2. Result of Biostimulation and Biotransformation Experiments. Ground Water. doi
  3. (1991). A Field Evaluation of In-situ Biorestoration of Chlorinated Ethenes: Part 3. Studies of Competitive Inhibition. Ground Water. doi
  4. (1986). American Chemical Society,
  5. (1990). Anaerobic In-Situ Treatment of Chlorinated Ethenes.
  6. (1995). Anaerobic Transformation of Chlorinated Aliphatic Hydrocarbons in a Sand Aquifer Based on Spatial Chemical Distributions. Water Res. doi
  7. (1981). Aquatic Chemistry. 2nd Ed.
  8. (1985). Biodegradation of Contaminants in the Subsurface. In Groundwater
  9. (1989). Biological Reductive Dechlorination of Tetrachloroethylene and Trichloroethylene to Ethylene under Methanogenic Conditions.
  10. (1988). Biotic and Abiotic Transformation of Halogenated Aliphatic Compounds,
  11. (1991). Biotransformation of Cis-1,2-dichloroethylene in Aquifer Material from Picatinny Aresenal, Morris County,
  12. (1985). Biotransformation of Tetrachloroethylene to Trichloroethylene, Dichloroethylene, Vinyl Chloride, and Carbondioxide under Methanogenic Conditions.
  13. (1995). Characterization of an 112-Utilizing Enrichment Culture That Reductively Dechlorinated Tetrachloroethene to Vinyl Chloride and Ethene in the Absence of Methanogenesis and Acetogenesis.
  14. (1994). Cometabolic Degradation of Trichloroethylene by Pseudomonas cepacia G4 in a Chemostat with Toluene. Appl Environ Microbio.
  15. (1995). Continuous-Flow and Batch Column Studies of Anaerobic Carbon Tetrachloride Biotransformation on Hanford Soil. Thesis of doi
  16. (1984). Dissimilatory Sulfate-or Sulfur- Reducing Bacteria. Bergey's manual of systematic bac. vol.1 . doi
  17. (1991). Effects of Toxicity, Aeration, and Reductant Supply on Trichloroethylene Transformation by a Methanotrophic Mixed Culture.
  18. (1992). Evaluation of Enhanced in Situ Aerobic Biodegradation of Trichloroethylene, and Cis-and-trans-1,2-Dichloroethylene by Phenol Utilizing Bacteria. Abstract:
  19. (1991). Field and Laboratory Evidence of In-Situ Biotransformation of Tetrachloroethene to Ethene and Ethane at a Chemical Transfer Facility
  20. (1994). Ground-Water Treatment for Chlorinated Solvents. Handbook of Bioremediation. Robert S. Kerr Environmental Research Laboratory.
  21. (1991). Groundwater Chemicals Field Guide.
  22. (1994). in: Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds Ed.: Hinchee, doi
  23. (1988). Inhibition and Stimulation of Trichloroethylene Biodegradation in Microaerophilic Microcosms. Environ Toxic Chem 7:889-895. doi
  24. (1992). Maximal Biodegradation Rates of Chloroform and Trichloroethylene in Anaerobic Treatment.
  25. (1993). Microcosm and In Situ Field Studies of Enhanced Biotransformation of Trichloroethylene by Phenol-Utilizing Microorganisms. doi
  26. (1992). Movement and Transformation of Halogenated Aliphatic Compounds in Natural Systems. In:
  27. (1989). National Primary and Secondary Drinking Water Regulation: propose rule
  28. (1992). Natural Anaerobic Treatment of a TCE Plume at St.
  29. (1991). Reductive Dechlorination of High Concentration of Tetrachloroethene to Ethene by an Anaerobic Enrichment Culture in the Absence of Methanogenesis. doi
  30. (1991). Reductive Dechlorination of Tri- and Tetrachloroethylenes Depends on Transition from Aerobic to Anaerobic Conditions.
  31. (1991). Reductive Dechlorination of Tri-and Tetrachloroethylene by Nonmethanogenic Enrichment Cultures.
  32. (1992). Stimulation of Reductive Dechlorination of Tetrachloroethene in Anaerobic Aquifer Microcosms by Addition of Short-chain Organic Acids or Alcohol. doi
  33. (1991). Stimulation of the Reductive Dechlorination of Tetrachloride doi
  34. (1990). Tetrachloroethene Transformation to Trichloroethene and Cis-1,2-dichloroethene by Sulfate-Reducing Enrichment Cultures.
  35. (1995). The Role of Iron in Enhancing Anaerobic Toluene Degradation in Sulfate-Reducing Enrichment Cultures. doi
  36. (1974). The Sulfur Cycle,
  37. (1991). Transformation of Trichloroethylene by Sulfatereducing Cultures Enriched from a Contaminated Subsurface Soil. Appl Microbiol Biotechnol. doi
  38. (1981). Treatment Techniques for Controlling Trihalomethanes in Drinking Water. Drinking Water Research Div.,
  39. (1993). Trichloroethylene Concentration Effects on Pilot Field-Scale In-Situ Groundwater Bioremediation by Phenol-Oxidizing Microorganisms. doi
  40. (1984). Water Works Assoc.

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