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Revealing biases inherent in recombination protocols

By Javier F Chaparro-Riggers, Bernard LW Loo, Karen M Polizzi, Phillip R Gibbs, Xiao-Song Tang, Mark J Nelson and Andreas S Bommarius

Abstract

Conclusion: By comparing different recombination scenarios, this study expands on existing recombination knowledge and sheds new light on known biases, which should improve library-creation efforts. It could be shown that the recombination-dependent PCR is an easy to perform alternative to DNA shuffling.

Topics: Methodology Article
Year: 2007
DOI identifier: 10.1186/1472-6750-7-77
OAI identifier: oai:smartech.gatech.edu:1853/44635
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