Can lateral mobility be restored along a highly domesticated low-energy gravel-bed river?

International audienc

Nanoscale characterization of collagen structural responses to in situ loading in rat Achilles tendons

International audienceThe specific viscoelastic mechanical properties of Achilles tendons are highly dependent on the structural characteristics of collagen at and between all hierarchical levels. Research has been conducted on the deformation mechanisms of positional tendons and single fibrils, but knowledge about the coupling between the whole tendon and nanoscale deformation mechanisms of more commonly injured energy-storing tendons, such as Achilles tendons, remains sparse. By exploiting the highly periodic arrangement of tendons at the nanoscale, in situ loading of rat Achilles tendons during small-angle X-ray scattering acquisition was used to investigate the collagen structural response during load to rupture, cyclic loading and stress relaxation. The fibril strain was substantially lower than the applied tissue strain. The fibrils strained linearly in the elastic region of the tissue, but also exhibited viscoelastic properties, such as an increased stretchability and recovery during cyclic loading and fibril strain relaxation during tissue stress relaxation. We demonstrate that the changes in the width of the collagen reflections could be attributed to strain heterogeneity and not changes in size of the coherently diffracting domains. Fibril strain heterogeneity increased with applied loads and after the toe region, fibrils also became increasingly disordered. Additionally, a thorough evaluation of radiation damage was performed. In conclusion, this study clearly displays the simultaneous structural response and adaption of the collagen fibrils to the applied tissue loads and provide novel information about the transition of loads between length scales in the Achilles tendon

Consumer resistance in services

International audienc

Towards comprehensive identification of pesticide degradation products following thermal processing below and above 120 °C: A review

International audienceCharacterising pesticide residues from a qualitative and quantitative point of view is key to both risk assessment in the framework of pesticide approval and risk management. In the European Union (EU), these concerns are addressed during the evaluation of active substances at the European level prior to marketing authorisation. In the framework of this review, we will focus on one specific item of the residue section, namely the effect of process (industrial or domestic transformation of the raw commodities) on the nature of the residue in food. A limited number of hydrolysis conditions defined by three parameters (temperature, pH and time) are set to be “representative of the most widely used industrial and domestic food processing technologies”. These hydrolysis conditions, however, do not cover processes at temperatures higher than 120 °C, such as cooking with a conventional oven or in a pan, frying or using a microwave oven

Human type I IFN deficiency does not impair B cell response to SARS-CoV-2 mRNA vaccination

International audienceInborn and acquired deficits of type I interferon (IFN) immunity predispose to life-threatening COVID-19 pneumonia. We longitudinally profiled the B cell response to mRNA vaccination in SARS-CoV-2 naive patients with inherited TLR7, IRF7, or IFNAR1 deficiency, as well as young patients with autoantibodies neutralizing type I IFNs due to autoimmune polyendocrine syndrome type-1 (APS-1) and older individuals with age-associated autoantibodies to type I IFNs. The receptor-binding domain spike protein (RBD)–specific memory B cell response in all patients was quantitatively and qualitatively similar to healthy donors. Sustained germinal center responses led to accumulation of somatic hypermutations in immunoglobulin heavy chain genes. The amplitude and duration of, and viral neutralization by, RBD-specific IgG serological response were also largely unaffected by TLR7, IRF7, or IFNAR1 deficiencies up to 7 mo after vaccination in all patients. These results suggest that induction of type I IFN is not required for efficient generation of a humoral response against SARS-CoV-2 by mRNA vaccines

Devenir des microplastiques dans les sols – quels indicateurs des sources, teneurs et impacts pour la qualité et la santé des sols ? (Projet PlastiSol)

International audienc

Can lateral mobility be restored along a highly domesticated low-energy gravel-bed river?

International audienceFluvial engineering works such as weirs, rip-rap, groynes, and dykes have constrained for decades and more the lateral mobility of rivers, one of the key drivers of aquatic and riparian diversity. Preserving or restoring a sufficient space for river mobility has therefore become a major river management focus. Because the success and relevance of management actions are conditioned by the level of energy and sediment supply of rivers, such actions are generally considered unsuitable for low-energy rivers. However, some low-energy rivers have numerous ancient engineering works along their length, especially bank protections, suggesting a potential capacity for bed migration. In this context, it is essential to determine to what extent planform dynamics is disturbed, and if lateral mobility can be restored. Herein, a case study was done on a 146 km stretch of the lowenergy meandering gravel-bed Cher River (France). The goal of the study was to estimate the remnant shifting capacity, identify the factors controlling the location and intensity of lateral erosion, determine the potential for preserving and restoring lateral mobility, and examine management measures that could be implemented to this end. For that, field surveys, analysis of existing databases, aerial photographs, and laser imaging detection and ranging digital elevation model (LiDAR DEM) data were combined. The study revealed a strong longitudinal fragmentation of the river, with most of it laterally constrained due to the presence of anthropogenic structures such as bank protections, former gravel pits in the alluvial plain, bridges, and weirs. The river is now composed of a string of constrained and unconstrained reaches, and the space available for river shifting has been dramatically reduced. Due to these fluvial engineering works and anthropogenic legacies, the potential for lateral movement of the riverbed, and, therefore, diversification of riparian and aquatic habitats, is limited. Furthermore, lateral mobility could be preserved or restored only for very short sections of the river. It is therefore highly unlikely that good ecological status could be achieved on the entire river corridor through removal of bank protections. Nevertheless, a possible solution could be combining bank protection removals with a series of gravel augmentations close to each other

The older, the better: Ageing improves the efficiency of biochar-compost mixture to alleviate drought stress in plant and soil

International audienc

Force tuning through regulation of clathrin-dependent integrin endocytosis

International audienceIntegrin endocytosis is essential for many fundamental cellular processes. Whether and how the internalization impacts cellular mechanics remains elusive. Whereas previous studies reported the contribution of the integrin activator, talin, in force development, the involvement of inhibitors is less documented. We identified ICAP-1 as an integrin inhibitor involved in mechanotransduction by co-working with NME2 to control clathrin-mediated endocytosis of integrins at the edge of focal adhesions (FA). Loss of ICAP-1 enables β3-integrin-mediated force generation independently of β1 integrin. β3-integrin-mediated forces were associated with a decrease in β3 integrin dynamics stemming from their reduced diffusion within adhesion sites and slow turnover of FA. The decrease in β3 integrin dynamics correlated with a defect in integrin endocytosis. ICAP-1 acts as an adaptor for clathrin-dependent endocytosis of integrins. ICAP-1 controls integrin endocytosis by interacting with NME2, a key regulator of dynamin-dependent clathrin-coated pits fission. Control of clathrin-mediated integrin endocytosis by an inhibitor is an unprecedented mechanism to tune forces at FA

Asymptotics of the Inertia Moments and the Variance Conjecture in Schatten Balls

International audienceWe study the first and second orders of the asymptotic expansion, as the dimension goes to infinity, of the moments of the Hilbert-Schmidt norm of a uniformly distributed matrix in the p-Schatten unit ball. We consider the case of matrices with real, complex or quaternionic entries, self-adjoint or not. When p > 3, this asymptotic expansion allows us to establish a generalized version of the variance conjecture for the family of p-Schatten unit balls of self-adjoint matrices