The first record of Lysmata rauli Laubenheimer and Rhyne, 2010 (Decapoda: Caridea: Lysmatidae) from the tropical eastern Atlantic

Here we report the first record of the shrimp Lysmata rauli Laubenheimer and Rhyne, 2010 in the eastern Atlantic, from the intertidal at Kere Island, Bijag & oacute;s archipelago, Guinea-Bissau.MARAFRICA (AGA-KHAN/540316524/2019); EU-H20202 854248 (Tropibio)

Endoscopy‐related musculoskeletal injuries: a systematic review and meta‐analysis on prevalence, risk factors and prevention

BackgroundEndoscopy-related musculoskeletal injuries (ERIs) are a major occupational hazard, impacting career longevity and personal well-being.ObjectiveThis systematic review and meta-analysis aimed to update and expand on previous findings by assessing prevalence, risk factors and management of ERIs among endoscopists.MethodsFollowing PRISMA guidelines, we systematically searched MEDLINE, Web of Science and Scopus for relevant studies published since the last comprehensive review. A manual search of the references of relevant manuscripts was also performed. Outcomes of interest included the prevalence of ERIs, common pain syndromes, risk factors, and preventive or treatment strategies. Studies' quality was assessed using the National Institutes of Health (NIH) Quality Assessment Tool.ResultsThirty studies were included, incorporating data from 7646 gastrointestinal endoscopists. The pooled career-long prevalence of overall ERI was 62.5% (CI 52.6-71.8, I2 = 98%), including pain (67.5%; CI 46.4%-85.6%; I2 = 98%) and numbness (12.4%; 95% CI 6.6%-19.7%; I2 = 98%) syndromes. Among pain syndromes, the most affected areas were the hand (28.2%; CI 19.2%-38.2%; I2 = 99%), lower back (27.3%; CI 20.1%-35.2%; I2 = 97%), thumb (27.1%; CI 18.9%-37.7%; I2 = 99%) and neck (25.7%; CI 19.3%-32.7%; I2 = 98%). Higher procedural volume, years in practice and female gender were consistently reported as risk factors for ERIs. Concerning therapy, 41.8% of endoscopists used medications (CI 31.2%-52.8%; I2 = 94%), while 28.2% engaged in physical therapy (CI 18.2%-39.5%; I2 = 96%). Sick leave was reported by 13.8% of endoscopists (CI 7.9%-20.9%; I2 = 94%). Practice modifications to manage ERIs included adjusting monitor (45.5%, CI 22.2%-69.9%; I2 = 96%) and table (32.4%, CI 14.5%-53.5%; I2 = 97%) height, but also reducing the number of cases per endoscopy session (14.6%; CI 10.4%-19.4%; I2 = 72%).ResultsThirty studies were included, incorporating data from 7646 gastrointestinal endoscopists. The pooled career-long prevalence of overall ERI was 62.5% (CI 52.6-71.8, I2 = 98%), including pain (67.5%; CI 46.4%-85.6%; I2 = 98%) and numbness (12.4%; 95% CI 6.6%-19.7%; I2 = 98%) syndromes. Among pain syndromes, the most affected areas were the hand (28.2%; CI 19.2%-38.2%; I2 = 99%), lower back (27.3%; CI 20.1%-35.2%; I2 = 97%), thumb (27.1%; CI 18.9%-37.7%; I2 = 99%) and neck (25.7%; CI 19.3%-32.7%; I2 = 98%). Higher procedural volume, years in practice and female gender were consistently reported as risk factors for ERIs. Concerning therapy, 41.8% of endoscopists used medications (CI 31.2%-52.8%; I2 = 94%), while 28.2% engaged in physical therapy (CI 18.2%-39.5%; I2 = 96%). Sick leave was reported by 13.8% of endoscopists (CI 7.9%-20.9%; I2 = 94%). Practice modifications to manage ERIs included adjusting monitor (45.5%, CI 22.2%-69.9%; I2 = 96%) and table (32.4%, CI 14.5%-53.5%; I2 = 97%) height, but also reducing the number of cases per endoscopy session (14.6%; CI 10.4%-19.4%; I2 = 72%).ConclusionERIs are highly prevalent among international gastrointestinal endoscopists, and are linked to procedural volume, years in practice, and gender. Ergonomic training and workplace adaptations are essential to mitigate risks and support career sustainability.Trial RegistrationPROSPERO Registration: CRD4202453434

Transcriptional regulation of Synaptonemal complex assembly and disassembly during meiosis

Human female meiosis can take several decades to be concluded, as the oocytes enter meiosis before birth and stay arrested in prophase I in a dormant state until puberty. We aim to better understand the mechanisms responsible for the correct awakening of the oocyte, and the way they ensure successful meiotic progression and oocyte maturation. We and others showed that the prophase I-arrested Drosophila oocyte is transcriptionally quiescent for approximately 36 hours, and similarly to human oocytes, its reactivation is associated with a poorly understood global remodelling of its chromatin architecture. A screen for endogenously GFP-tagged transcription factors allowed the identification of Polycomb as being highly enriched in the oocyte chromatin and we will present our ongoing work related with the function of this protein complex and our attempts to visualize oocyte reactivation using live-cell imaging techniques. Additionally, the synaptonemal complex (SC) is a proteinaceous scaffold that is assembled between the paired homologous chromosomes during the onset of meiosis. The SC stabilizes chromosome pairing and is important for crossovers formation, recombination and accurate segregation of meiotic chromosomes. Timely expression of SC genes is essential for SC assembly and successful meiosis. However, SC components have an intrinsic tendency to self-organize into alternative repetitive structures (polycomplexes), being potentially deleterious for meiosis and gametogenesis. In this work, we show that Sfmbt, is required to avoid excessive expression of SC genes during prophase I and consequently formation of polycomplexes during SC disassembly. Overexpression of Corona and depletion of other Polycomb group proteins are similarly associated to polycomplexes formation during SC disassembly. These polycomplexes are highly dynamic and have a well-defined periodic structure. Further confirming the importance of Sfmbt for female gametogenesis, germ line depletion of this protein is associated to significant metaphase I defects and a reduction of female fertility.meiose feminina humana pode levar várias décadas para ser concluída, pois os oócitos entram na meiose antes do nascimento e permanecem dormentes na prófase I até a puberdade. Neste trabalho procurámos compreender os mecanismos responsáveis pelo correto despertar do oócito e a forma como garantem a progressão e maturação meiótica. Foi demonstrado que o oócito de Drosophila preso na prófase I é transcricionalmente quiescente aproximadamente 36 horas e, de maneira semelhante aos oócitos humanos, a reativação está associada à remodelação global da arquitetura da cromatina. Usufruindo de uma seleção de fatores de transcrição marcados com GFP endógenos permitiu identificámos o Polycomb altamente enriquecido na cromatina do oócito e procurámos descobrir a função desta proteína bem como a visualizar oócitos através de técnicas de imagem ex vivo. Mais ainda, o complexo sinaptonémico (CS) é uma estrutura proteica que se forma entre os cromossomas homólogos durante o início da meiose. O CS estabiliza o emparelhamento de cromossomas sendo importante para a formação de crossingovers, recombinação e segregação dos cromossomas meióticos. A expressão correta dos genes do CS é essencial para a formação do CS e para o sucesso meiótico. No entanto, estes componentes têm uma tendência intrínseca de se auto-organizarem em estruturas repetitivas alternativas de ordem superior (policomplexos), sendo potencialmente perigosos para a meiose e a gametogénese. Neste trabalho, mostramos que o Sfmbt é necessário para evitar a expressão excessiva destes genes e, consequentemente, a formação de policomplexos durante a desmontagem do SC. A sobre expressão de Corona e a depleção de outras proteínas do grupo Polycomb estão também associadas à formação de policomplexos durante a desmontagem do SC. Estes policomplexos são altamente dinâmicos e possuem uma estrutura periódica bem definida. A depleção de Sfmbt está também associada com defeitos de metafase I e a uma redução da fertilidade feminina

Estudo de mercado: Laranja do Algarve

Este relatório sintetiza os resultados de um estudo de mercado, com recurso a um questionário, sobre a Laranja do Algarve

Statement of Peer Review

In submitting conference proceedings to Proceedings, the volume editors of the proceedings certify to the publisher that all papers published in this volume have been subjected to peer review administered by the volume editors. The reviews were conducted by expert referees, and to the professional and scientific standards expected of a proceedings journal.N/

Novel field experiment on alkalinity enhancement in intertidal environments—a trailblazer for natural climate solutions

One recently proposed approach to reduce atmospheric CO2 concentrations is marine alkalinity enhancement. This technique increases the CO2 uptake capacity of seawater through weathering of fine-grained (mafic) rocks and minerals in marine environments. The weathering process has been extensively tested in laboratory studies and verified by numerical models. Field experiments scaling the CO2 uptake under natural conditions are still lacking. In a methodological approach, a novel in situ experiment was designed and installed in a salt marsh at Ria Formosa coastal lagoon, southern Portugal. The experiment comprised deployments of different sizes of olivine and basalt substrates, and a control site, which were tidally submerged twice a day. A monthly monitoring scheme of supernatant and porewater properties from each deployment and control site was established, and procedures for temperature, salinity, oxygen, pH, total alkalinity, nutrient, and trace metal analyses were defined. This paper is devoted to the methods and describes the design, a protocol for the analyses, and an evaluation of experimental performance and reliability. Data from the first 6 months are presented for validation of the experiment. They demonstrated elevated total alkalinity in water samples, mostly in porewater after the deployments, while salinity, oxygen, and pH reflect the control conditions. Significant alkalinity differences were observed between the treatments and the natural background conditions monitored at the control site, during the 6 months of the experiment. The methodological approach is presented with strengths, limitations, and recommendations for an upscaling as CO2 removal measure, servicing, and subsequent investigations.PTDC/CTA-CLI/1065/2021; CEECINST/00052/2021/CP2792/CT0012; CEECINST/00052/2021/CP2792/CT0007; CEECINST/00146/2018/CP1493/CT0002; S-026426/202

Comparison of different microalgae biomass typologies used in rotifers enrichment for zebrafish (Danio rerio) larvae nutrition

The use of enriched rotifers with industrially produced microalgae represents a valuable tool for the enhancement of zebrafishlarval nutrition and increased biological performance. Currently, a monoculture of microalgal species (Nannochloropsis sp.) inform of liquid paste is routinely used for rotifers enrichment for zebrafish larvae feeding; however, the most adequate typology (i.e.,paste or freeze-dried) of the industrially produced microalgal biomass is still controversial. This work aimed to compare the effectsof rotifers enriched with three different industrially produced microalgae species (i.e., Nannochloropsis oceanica, Tetraselmis chui,and Tisochrysis lutea) using paste and freeze-dried powder. Enriched rotifers were provided as feed during larval growth and theimpact on growth and survival was evaluated. The use of enriched rotifers with both paste or freeze-dried microalgae improvedgrowth compared to larvae fed exclusively with commercial microdiet. Larvae fed rotifers enriched with N. oceanica and T. chuiattained higher weight and length both at 15 and 30 days postfertilization (dpf ), while the use of microalgae in paste contributed togreater larvae lengths when compared to freeze-dried. The experimental results in this study revealed that N. oceanica and T. chuiin paste are the most suitable microalgae forms to be used in zebrafish larvae nutrition and in the improvement of enrichmentmethodologies for rotifers.31/SI/2017/39896; 2022.10077.BDAN

Off the shelf: regulation of fish blood pH through ionocyte membrane remodelling and protein translocation without additional synthesi

Ocean acidification (OA), together with warming and deoxygenation, forms the “deadly trio,” a combination that reduces ecosystems’ resilience, makes marine biodiversity vulnerable, and is considered a main contributor to global mass extinctions in the Earth’s history (1)

Role of ancillary techniques in the differential diagnosis of salivary gland carcinomas

Paiva-Correia A, Hellquist H, Apolónio J, Castelo-Branco P. Role of ancillary techniques in the differential diagnosis of salivary gland carcinomas. The diagnosis of salivary gland carcinomas (SGC) rests mainly on histology, but immunohistochemical and molecular investigations are often necessary for differential diagnosis. This review is primarily aimed as a tool for pathologists in non-specialised head and neck hospitals who encounter a limited number of SGC annually. The use of testing an initial antibody panel, which may comprise both positive and negative expression for a suspected entity, and examples of different panels are outlined. We also focused on acinic cell carcinoma (AcCC), which is positive for DOG1 and negative for mammaglobin, whilst secretory carcinoma (SC) is positive for mammaglobin and negative for DOG1. In addition, the exclusive expression of androgen and HER2 in salivary duct carcinoma (SDC) and its use for differential diagnosis are also addressed. This review also highlights the particularities of mucoepidermoid carcinoma (MEC) and its negativity for S100 and SOX10, which distinguishes it from some of its mimics. In laboratories with limited access to antibodies for SGC, we recommend inclusion of mammaglobin. The use of molecular techniques for the diagnosis of MEC (MAML2), SC (ETV6), adenoid cystic carcinoma (MYB), and AcCC (NR4A3) is discussed. We highlight the role of commonly available antibodies for the histological classification of SGC

West Side Story: regional inter‐troop variation in baboon bark‐stripping at gorongosa national park, Mozambique

Objectives: Baboons possess sophisticated physical and social cognitive abilities; hence, the lack of evidence to date of large-scale behavioral variation in these primates is puzzling. Here we studied a candidate for such variation—the stripping of barkfrom Acacia robusta trees for consumption of the sap and soft tissue underneath—in Gorongosa National Park, Mozambique.Materials and Methods: We surveyed an area inhabited by ~60 troops of chacma baboons, recording the availability andcharacteristics of the target trees, as well as the presence or absence of bark-stripping at 45 habitat plots distributed across a gridcovering an area of ~300 km 2 .Results: Camera traps confirmed the presence of baboons at all habitat plots, and we identified regional clumping in the distri-bution of the behavior, a pattern consistent across two consecutive years. Proportion and mean height/width of A. robusta did notpredict whether bark-stripping behavior was present at a given site, nor did broader ecological variables such as habitat type anddistance to the nearest water source. However, stripping sites had significantly higher numbers of A. robusta than non-strippingsites, and within a given bark-stripping site, baboons preferred to strip taller and wider trees among those available.Discussion: The prominent geographical clustering we uncovered may have been driven by opportunity (i.e., the prevalenceof A. robusta at a given site), but is also consistent with a possible (non-mutually exclusive) cultural interpretation. We proposeavenues for future research on Gorongosa's baboons to better quantify the relative contributions of ecology, genetics, and social.CEECIND/01937/2017; ANID/FONDECY