Multiple online-HPLC methodologies and biological properties of leaves and stem barks extracts of Chrysanthellum indicum

Chrysanthellum indicum, an annual herb with yellow flowers, is used as a functional food in Asia and Africa for health purposes. In this study, we thoroughly investigated the characterization of phytochemicals within C. indicum's ethyl acetate, water, and methanolic extracts from both its bark and leaves. This was achieved through the utilization of the Folin Ciocalteu and aluminum trichloride assays. Moreover, the antioxidant properties of these extracts were comprehensively assessed employing microplate-based assays including ABTS, DPPH, CUPRAC, FRAP, MCA, and PBD, alongside online HPLC-based assays namely ABTS, DPPH, CUPRAC, and FRAP. Furthermore, the inhibitory activity against a range of enzymes, including AChE, BChE, tyrosinase, α-amylase, and α-glucosidase, was ascertained. The tested ethyl acetate extracts from bark and leaves had higher content of total phenolic and flavonoid compared to methanol and water extracts. Additionally, the bark extract exhibited superior antioxidant properties in the in vitro antioxidant assays. Meanwhile, in the realm of online HPLC antioxidant analysis, an effective method has been discovered for swiftly determining the individual antioxidant capacity of compounds within both standard solutions and extract. Within the entirety of the samples subjected to scrutiny, rutin surfaced as the foremost antioxidant, exhibiting its prominence at wavelengths of 280 nm, 595 nm, and 450 nm. Following suit, quercetin emerged as the second most abundant antioxidant, noticeable at wavelengths of 517 nm and 734 nm, both in leaves and bark extracts. Significantly, the extracts derived from both bark and leaves demonstrated noteworthy effectiveness in inhibiting the tyrosinase enzyme. Thus, our study indicates that Chrysanthellum indicum could serve as a promising reservoir of natural bioactive compounds, making it suitable for the formulation of health-promoting applications

Characterization and comparison of insulinoma tumor model and pancreatic damage caused by the tumor, and identification of possible markers

Abstract: Insulinoma is a neuroendocrine tumor. It arises from the uncontrolled proliferation of pancreatic β cells. In this study, we created an insulinoma tumor model in nude mice. INS-1 cells were injected in two different ways, subcutaneously (S.C.) or intraperitoneally (I.P.). Body weight, tumor weight, and size were measured. ELISA kits were used analyze to Glucose, insulin, and CA19-9 levels in serum, pancreas, and tumor tissues. KCNN4, KCNK1, GLUT2, IR, HSP70, HSF1, and HSP90 levels were analyzed by western blotting of membrane and/or cytosolic fractions of tumor and pancreas tissue. Tumor formation occurred in nude mice, but it did not occur in Wistar albino rats. The tumor has neuroendocrine cell morphology. Insulin and CA19-9 levels increased in pancreas tissue. In tumor tissue, KCNN4 levels were higher in both membrane and cytosolic fractions, while KCNK1 levels were lower in the membrane fraction of the S.C. group. HSP70 levels were also lower in the S.C. group. In pancreas tissue, KCNK1 levels were lower in the membrane fraction of the S.C. and I.P. groups. GLUT2 levels increased in both groups according to the control group, while IR levels decreased in the S.C. group compared to the control group. However, HSF1 levels increased in the I.P. group, while HSP90 decreased in the S.C. group in pancreatic tissues. The S.C. group is a more suitable insulinoma tumor model. KCNN4, KCNK1, and HSP70 proteins may be important biomarkers in the diagnosis and treatment of insulinoma.Istanbul Universit

Effect of nonnutritive sucking on oral feeding in neonates with perinatal asphyxia: A randomized controlled trial

AIMS: The effect of nonnutritive sucking (NNS) stimulation is unclear in infants with perinatal asphyxia. Thus, the aim of this study was to assess the effect of NNS stimulation on oral intake, discharge time, and early feeding skills in infants with perinatal asphyxia. DESIGN: A randomized controlled study was conducted. METHOD: Of the 94 infants, 47 were included in the experimental group and given NNS stimulation once a day before tube feeding by a speech-language therapist (SLT) in addition to hypothermia treatment. Infants' feeding performances on the days of first oral intake and discharge were evaluated with the Early Feeding Skills Scale (EFS). RESULTS: The time from tube feeding to oral intake was significantly lower in the experimental group compared to the control group (p .05). CONCLUSIONS: The findings indicated that the NNS stimulation positively affected oral intake and early feeding skills in infants with perinatal asphyxia, as in preterms. However, NNS stimulation had no significant effect on discharge and weight gain in infants with asphyxia. This finding may be attributed to other factors. It is recommended to use NNS by an SLT in a neonatal intensive care unit within a multidisciplinary team to accelerate the transition to oral feeding and improve feeding skills in infants with perinatal asphyxia. Further studies on the effect of NNS stimulation in infants with perinatal asphyxia are needed to corroborate its effects on discharge time and weight gain

Evaluating the impact of aerobic and resistance green exercises on the fitness, aerobic and intrinsic capacity of older individuals

Objective: Our study determined the impact of 12-week aerobic exercise (AE) and aerobic + resistance exercises (AE + RE) within the green exercise concept, on senior fitness, aerobic capacity, and intrinsic capacity (IC). Methods: The study was a multicenter, randomized controlled study conducted at two senior living facilities with individuals aged 65 and above whose cardiorespiratory and musculoskeletal conditions are suitable for moderate exercise and who have normal cognition levels. Block randomization was applied to 96 participants in a ratio of 1:1:1 to be assigned to AE, AE + RE, and control (C) groups. Intervention groups received exercise sessions led by physiotherapists within the senior living facilitiy gardens, with a frequency of once a week for 50 min, for 12 weeks. Also, they were prescribed additional exercise sessions on two additional days of the week. At the commencement of the study and 12th week, shuttle walking test, senior fitness test (SFT), intrinsic capacity assessment (with Timed Up and Go test, Mini Mental State Examination, Geriatric Depression Scale-15, Mini Nutritional Assessment, handgrip strength test) was conducted of all participants. The primary outcome was the Z score of IC, secondary outcomes were VO2max and SFT subparameters. The study was registered in the Protocol Registry and Results System (Clinicaltrials.gov PRS) with the registration number NCT05958745. Results: 90 participants successfully completed the study, with 30 individuals in each of the AE, AE + RE, and C groups. By the end of the 12th week, the arm curl score was significantly higher in the AE + RE compared to the C (mean difference: 3.96, 95 % CI= 2.47 to 5.46, p = 0.01). There were significant differences in chair stand, two-minute step, 8-foot up-and-go, chair sit and reach, and back scratch tests in both AE and AE + RE compared to C. AE and AE + RE exhibited significantly higher shuttle test distances and VO2max values compared to the C (p < 0.0001). AE + RE achieved a significantly higher total IC score than the C (mean difference: 0.59, %95 CI= -0.07 to 1.26, p = 0.025). Conclusion: In this study within the green exercise concept, both AE and AE + RE led to similar improvements in strength, flexibility, mobility, endurance, and aerobic capacity. Notably, AE + RE demonstrated an additional benefit by increasing the total IC, while AE alone did not exhibit the same effect.Ministry of Family and Social Service

A new trend in cryptographic information security for industry 5.0: A systematic review

Malicious assaults and information leakage have grown in importance in practically every area of information and communication technology (ICT) in recent years. Enterprise ledger organization, preservation, security, and protection are all greatly aided by information security (IS). To maintain their positions in the market, industries must safeguard their data and other vital assets. The main goals of this paper's systematic review, which covers the entire process of privacy protection and security, are to introduce cryptographic IS for Industry 5.0 (the Fifth Industrial Revolution) as a comprehensive solution that provides regulatory compliance and IS policies in addition to the industrial goals. The review covers the four main areas: 1) recognizing the need for frameworks to be developed for a secure Industry 5.0 lifecycle; 2) emphasizing security guidelines, procedures, and countermeasures for Industry 5.0; 3) applying control, access, and availability in real-time; and 4) proposing a futuristic architecture for industrial information security. Furthermore, the analysis of previously published state-of-the-art techniques is presented in this paper. These techniques survive with various limitations and challenges, including intercommunication exchange, fine-grained control access, and interconnectivity-related issues, which affect the adoption of an IS in industrial programs. At the end of this systematic review, we investigated a few open research problems and mentioned those that are involved in the design of cryptographic re-encryption enabled IS for future Industry 5.0 developments.Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2024R236), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabi

Prioritizing the indicators of energy performance management: a novel fuzzy decision-making approach for G7 service industries

Ensuring energy performance management is important in many ways, such improvement of energy efficiency and decrease of energy costs are reduced. There are various indicators of the effectiveness of energy performance management of buildings. Due to this situation, businesses need to make the necessary improvements for the development of these factors. Nonetheless, these actions cause an increase in the costs of the companies. Hence, among these actions, the more important ones need to be identified. Owing to this issue, businesses can use their limited budgets for more priority indicators. The purpose of this study is to evaluate the main indicators of energy performance management systems. In this way, a new model is proposed to make a priority analysis for the hospitals. Firstly, five indicators of energy performance management systems are selected by considering ISO 50006 standards. Furthermore, these indicators are weighted by using Spherical fuzzy CRITIC. Secondly, G7 countries are examined with fuzzy RATGOS technique. Identification of the most significant indicators of the energy performance systems is an important novelty of this study. The most significant methodological novelty of this study is proposing a new technique to the literature named RATGOS. It is understood that energy efficiency is the most crucial indicator of energy performance management. Furthermore, it is also identified that France is the most successful G7 economy with respect to the energy performance management. Japan and United States have also high performance in this respect. It is recommended that necessary actions should be taken to increase energy efficiency. By conducting an energy audit, energy consumption data is analyzed so that energy losses and inefficiencies can be detected. This assessment provides opportunities for energy efficiency and helps identify improvement strategies.Ministry of Culture and Innovation of Hungary ; Russian Science Foundation ; Nemzeti Kutatási, Fejlesztési és Innovaciós Ala

Single agent vemurafenib or rituximab-vemurafenib combination for the treatment of relapsed/refractory hairy cell leukemia, a multicenter experience

Background: Hairy cell leukemia (HCL) is a rare mature B-cell malignancy that is primarily treated with purine analogues. However, relapse remains a significant challenge, prompting the search for alternative therapies. The BRAF V600E mutation prevalent in HCL patients provides a target for treatment with vemurafenib. Patients and methods: This multicenter retrospective study included nine patients with relapsed/refractory (R/R) HCL from six different centers. Patient data included demographics, prior treatments, clinical outcomes, and adverse events. Results: Patients received different treatment regimens between centers, including vemurafenib alone or in combination with rituximab. Despite the differences in protocols, all patients achieved at least a partial response, with seven patients achieving a complete response. Adverse events were generally mild with manageable side effects. The absence of myelotoxic effects and manageable side effects make BRAF inhibitors attractive, especially for patients ineligible for purine analogues or those with severe neutropenia. Conclusion: Single agent vemurafenib or in combination with rituximab appears to be a promising therapeutic option for R/R HCL. Further research is needed to establish standardized treatment protocols and to investigate long-term outcomes

Optimizing percutaneous pulmonary valve implantation with patient-specific 3D-printed pulmonary artery models and hemodynamic assessment

Background: Percutaneous pulmonary valve implantation (PPVI) has emerged as a less invasive alternative for treating severe pulmonary regurgitation after tetralogy of Fallot (TOF) repair in patients with a native right ventricular outflow tract (RVOT). However, the success of PPVI depends on precise patient-specific valve sizing, the avoidance of oversizing complications, and optimal valve performance. In recent years, innovative adaptations of commercially available cardiovascular mock loops have been used to test conduits in the pulmonary position. These models are instrumental in facilitating accurate pulmonic valve sizing, mitigating the risk of oversizing, and providing insight into the valve performance before implantation. This study explored the utilization of custom-modified mock loops to implant patient-specific 3D-printed pulmonary artery geometries, thereby advancing PPVI planning and execution.Material and Methods: Patient-specific 3D-printed pulmonary artery geometries of five patients who underwent PPVI using Pulsta transcatheter heart valve (THV) (R) were tested in a modified ViVitro pulse duplicator system (R). Various valve sizes were subjected to 10 cycles of testing at different cardiac output levels. The transpulmonary systolic and regurgitation fractions of the valves were also recorded and compared.Results: A total of 39 experiments were conducted using five different patient geometries and several different valve sizes (26, 28, 30, and 32 mm) at 3, 4, and 5 L/min cardiac output at heart rates of 70 beats per minute (bpm) and 60/40 systolic/diastolic ratios. The pressure gradients and regurgitation fractions of the tested valve sizes in the models were found to be similar to the pressure gradients and regurgitation fractions of valves used in real procedures. However, in two patients, different valve sizes showed better hemodynamic values than the actual implanted valves.Discussion: The use of 3D printing technology, electromagnetic flow meters, and the custom-modified ViVitro pulse duplicator system (R) in conjunction with patient-specific pulmonary artery models has enabled a comprehensive assessment of percutaneous pulmonic valve implantation performance. This approach allows for accurate valve sizing, minimization of oversizing risks, and valuable insights into hemodynamic behavior before implantation. The data obtained from this experimental setup will contribute to advancing PPVI procedures and offer potential benefits in improving patient outcomes and safety.TUSE

Comparison of liquid-based cytology and cell blocks prepared from cell remnants for diagnosis of cervical pathology

Background: Cervical cancer is a global public health problem with high mortality. Advances in screening programs for cervical cancer are considered key to eliminate cervical cancer. We aimed to examine the contribution of cell block analysis to the detection of epithelial cell abnormalities in cervical smear samples. Methods: A total of 559 patients with suspected cervical pathology were examined, and their samples were analyzed by both liquid-based cytology (LBC) and cell blocks. The biopsy results of 149 out of the 559 patients were obtained. Results: Of the 50 patients who were identified as HSIL by biopsy, only 12 were diagnosed as HSIL by the LBC method, 22 as LSIL, 12 as ASCUS, and 4 as ASC-H (p < 0.001). With the cell block analysis, results for these patients were: 20 HSIL, 17 LSIL, 7 NILM, 4 ‘unsatisfactory’, and 2 ASC cases (p < 0.001). LBC detected only 1 of the 10 patients with biopsy-diagnosed tumors, while 7 of these were defined as HSIL, 1 as ASCUS and 1 as AGC. The results of cell block analysis in patients with biopsy-diagnosed tumors were as follows: 7 HSIL, 1 tumor, 1 ASC and 1 LSIL. Conclusions: Cell block analysis might be superior to LBC in terms of diagnostic accuracy in cervical pathologies, particularly in the detection of HSIL. However, both methods were similarly poor in diagnosing tumors. Cell blocks may improve diagnostic accuracy and can be a complementary method to LBC, while having the advantage of revealing histological architecture

Knockdown of NEAT1 prevents post-stroke lipid droplet agglomeration in microglia by regulating autophagy

Background: Lipid droplets (LD), lipid-storing organelles containing neutral lipids like glycerolipids and cholesterol, are increasingly accepted as hallmarks of inflammation. The nuclear paraspeckle assembly transcript 1 (NEAT1), a long non-coding RNA with over 200 nucleotides, exerts an indispensable impact on regulating both LD agglomeration and autophagy in multiple neurological disorders. However, knowledge as to how NEAT1 modulates the formation of LD and associated signaling pathways is limited. Methods: In this study, primary microglia were isolated from newborn mice and exposed to oxygen-glucose-deprivation/reoxygenation (OGD/R). To further explore NEAT1-dependent mechanisms, an antisense oligonucleotide (ASO) was adopted to silence NEAT1 under in vitro conditions. Studying NEAT1-dependent interactions with regard to autophagy and LD agglomeration under hypoxic conditions, the inhibitor and activator of autophagy 3-methyladenine (3-MA) and rapamycin (RAPA) were used, respectively. In a preclinical stroke model, mice received intraventricular injections of ASO NEAT1 or control vectors in order to yield NEAT1 knockdown. Analysis of readout parameters included qRT-PCR, immunofluorescence, western blot assays, and behavioral tests. Results: Microglia exposed to OGD/R displayed a temporal pattern of NEAT1 expression, peaking at four hours of hypoxia followed by six hours of reoxygenation. After effectively silencing NEAT1, LD formation and autophagy-related proteins were significantly repressed in hypoxic microglia. Stimulating autophagy in ASO NEAT1 microglia under OGD/R conditions by means of RAPA reversed the downregulation of LD agglomeration and perilipin 2 (PLIN2) expression. On the contrary, application of 3-MA promoted repression of both LD agglomeration and expression of the LD-associated protein PLIN2. Under in vivo conditions, NEAT1 was significantly increased in mice at 24 h post-stroke. Knockdown of NEAT1 significantly alleviated LD agglomeration and inhibited autophagy, resulting in improved cerebral perfusion, reduced brain injury and increased neurological recovery. Conclusion: NEAT1 is a key player of LD agglomeration and autophagy stimulation, and NEAT1 knockdown provides a promising therapeutic value against stroke. Graphical abstract: [Figure not available: see fulltext.].Georg-August-Universität Göttingen ; China Scholarship Counci