Navigating disconnected social circles: experiences and personal networks of Latino/a adolescents in the U.S. rural South

Informed by Bronfenbrenner’s ecological model of development, this mixed-methods study’s aims are to: (1) describe rural Latino/a adolescents’ (N = 62) narratives and lived social experiences in the context of rurality, and (2) examine their personal networks to better understand their social interactions (subset of 30 adolescents). Rural Latino/a adolescents move in limited social circles and experience geographic, cultural, and social isolation due to immigration status problems, socioeconomic issues, racial discrimination, and family dynamics. This limitation is reflected by personal networks that tend to be homogenous in terms of ethnicity, age, and sociodemographic characteristics. School, although characterized by weak social ties often disconnected from community and family contacts, emerged as the dominant context of sociability where adolescents build their social identity outside the circle of dense family ties. Findings suggest a critical need for interventions to reduce isolation and enhance social connectedness between family, school, and rural community in this population

Appunti delle lezioni berlinesi di Karl Georg Bruns

La trascrizione degli appunti di Eugen Huber al corso di ‘Pandekten’ di Karl Georg Bruns tenutosi nel 1870 a Berlino costituisce un lavoro meritorio che mette a disposizione una fonte inedita: si tratta di una testimonianza importante non solo della formazione romanistica di Huber, padre del Codice civile svizzero, ma anche del ruolo di docente di Bruns, dalle cui lezioni emerge la consapevolezza del diritto romano quale base comune alla cultura giuridica europea.The transcription of Eugen Huber’s notes on Karl Georg Bruns’ ‘Pandekten’ course held in 1870 in Berlin constitutes a meritorious work that makes a previously unpublished source available: it is an important testimony not only of the romanistic training of Huber, the father of the Swiss Civil Code, but also of the teaching role of Bruns, from whose lectures emerges the awareness of Roman law as a common basis for European legal culture

Dalla "giurisprudenza Falciani" alla mancata (forse ancora per poco) attuazione di una disciplina sul tax whistleblowing

Nel corso degli anni diverse sono state le figure dei segnalatori fiscali, che mosse da intenti ed esigenze differenti, hanno portato alla nascita dei c.d. (tax) whistleblower. La perimetrazione di tali soggetti ha rappresentato un'enorme sfida sia a livello euro-unitario sia a livello nazionale, sfida cui ha partecipato in maniera fortemente attiva anche la giurisprudenza. L'ultimo approdo è stato rappresentato dal recepimento nell'ordinamento nazionale della dir. 23 ottobre 2019, n. 1937, di cui, ora, si attendono i (tanto sperati) frutti

Integrative approach for precise genotyping and transcriptomics of salt tolerant introgression rice lines

Rice is the most salt sensitive cereal crop and its cultivation is particularly threatened by salt stress, which is currently worsened due to climate change. This study reports the development of salt tolerant introgression lines (ILs) derived from crosses between the salt tolerant indica rice variety FL478, which harbors the Saltol quantitative trait loci (QTL), and the salt-sensitive japonica elite cultivar OLESA. Genotyping-by-sequencing (GBS) and Kompetitive allele specific PCR (KASPar) genotyping, in combination with step-wise phenotypic selection in hydroponic culture, were used for the identification of salt-tolerant ILs. Transcriptome-based genotyping allowed the fine mapping of indica genetic introgressions in the best performing IL (IL22). A total of 1,595 genes were identified in indica regions of IL22, which mainly located in large introgressions at Chromosomes 1 and 3. In addition to OsHKT1;5, an important number of genes were identified in the introgressed indica segments of IL22 whose expression was confirmed [e.g., genes involved in ion transport, callose synthesis, transcriptional regulation of gene expression, hormone signaling and reactive oxygen species (ROS) accumulation]. These genes might well contribute to salt stress tolerance in IL22 plants. Furthermore, comparative transcript profiling revealed that indica introgressions caused important alterations in the background gene expression of IL22 plants (japonica cultivar) compared with its salt-sensitive parent, both under non-stress and salt-stress conditions. In response to salt treatment, only 8.6% of the salt-responsive genes were found to be commonly up- or down-regulated in IL22 and OLESA plants, supporting massive transcriptional reprogramming of gene expression caused by indica introgressions into the recipient genome. Interactions among indica and japonica genes might provide novel regulatory networks contributing to salt stress tolerance in introgression rice lines. Collectively, this study illustrates the usefulness of transcriptomics in the characterization of new rice lines obtained in breeding programs in rice

Sull’interpretazione della clausola di rinnovo delle R&W al momento del Closing

La nota si confronta con le criticità interpretative poste dal rapporto fra contratto preliminare di compravendita di partecipazioni sociali e atto di trasferimento delle medesime, con particolare riferimento alle dichiarazioni e garanzie contenute nel primo e non ripetute nel secondo. Si evidenziano gli inconvenienti che derivano dall’uso di formule contrattuali di origine ‘‘aliena’’ in un contesto giuridico nel quale valgono diversi princip

Milk proteins as mastitis markers in dairy ruminants : a systematic review

Mastitis is one of the most impacting diseases in dairy farming, and its sensitive and specific detection is therefore of the greatest importance. The clinical evaluation of udder and mammary secretions is typically combined with the milk Somatic Cell Count (SCC) and often accompanied by its bacteriological culture to identify the causative microorganism. In a con- stant search for improvement, several non-enzymatic milk proteins, including milk amyloid A (M-SAA), haptoglobin (HP), cathelicidin (CATH), and lactoferrin (LF), have been investigated as alternative biomarkers of mastitis for their relationship with mammary gland inflammation, and immunoassay techniques have been developed for detection with varying degrees of success. To provide a general overview of their implementation in the different dairy species, we carried out a systematic review of the scientific literature using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines. Our review question falls within the type “Diagnostic test accuracy questions” and aims at answering the diag- nostic question: “Which are the diagnostic performances of mastitis protein biomarkers investigated by immunoassays in ruminant milk?”. Based on 13 keywords combined into 42 searches, 523 manuscripts were extracted from three scientific databases. Of these, 33 passed the duplicate removal, title, abstract, and full-text screening for conformity to the review ques- tion and document type: 78.8% investigated cows, 12.1% sheep, 9.1% goats, and 6.1% buffaloes (some included more than one dairy species). The most frequently mentioned protein was M-SAA (48.5%), followed by HP (27.3%), CATH (24.2%) and LF (21.2%). However, the large amount of heterogeneity among studies in terms of animal selection criteria (45.5%), index test (87.9%), and standard reference test (27.3%) resulted in a collection of data not amenable to meta-analysis, a common finding illustrating how important it is for case definitions and other criteria to be standardized between studies. Therefore, results are presented according to the SWiM (Synthesis Without Meta-analysis) guidelines. We summarize the main findings reported in the 33 selected articles for the different markers and report their results in form of comparative tables including sample selection criteria, marker values, and diagnostic performances, where available. Finally, we report the study limitations and bias assessment findings

Vitamin D status and SARS-CoV-2 infection in a cohort of kidney transplanted patients

Background: Recently the protective role of 25-hydroxyvitamin D (25(OH)D) against viral infections has been hypothesized. We evaluated the association between vitamin D status and SARS-CoV-2 infection susceptibility and severity in a cohort of kidney transplanted patients (KTxp). Methods: A total of 61 KTxp with SARS-CoV-2 infection (COV+) were matched with 122 healthy KTxp controls (COV−). Main biochemical parameters at 1, 6, and 12 months before SARS-CoV-2 infection were recorded. Vitamin D status was considered as the mean of two 25(OH)D measures obtained 6 ± 2 months apart during the last year. The severity of SARS-CoV-2 infection was based on the need for hospitalization (HOSP+) and death (D+). Results: 25(OH)D levels were lower in COV+ than in controls [19(12–26) vs. 23(17–31) ng/mL, p = 0.01]. No differences among the other biochemical parameters were found. The SARS-CoV-2 infection discriminative power of 25(OH)D was evaluated by ROC-curve (AUC 0.61, 95% CI 0.5–0.7, p = 0.01). 25(OH)D was not significantly different between HOSP+ and HOSP− [17(8–25) vs. 20(15–26) ng/mL, p = 0.19] and between D+ and D− [14(6–23) vs. 20(14–26) ng/mL, p = 0.22] and had no significant correlation with disease length. Conclusions: During the year preceding the infection, 25(OH)D levels were lower in COV+ KTxp in comparison with controls matched for demographic features and comorbidities. No significant association between vitamin D status and SARS-CoV-2 infection related outcomes was found

Pregnancy in juvenile idiopathic arthritis: maternal and foetal outcome, and impact on disease activity

Objective: This retrospective cohort study describes the modulation of disease activity during gestation and in the year following delivery as well as maternal and neonatal outcomes in a monocentric cohort of women with juvenile idiopathic arthritis (JIA).Methods: Disease activity was assessed using DAS28-CRP before conception and every 3 months during pregnancy and in the first year postpartum. The risk of complicated pregnancies was measured applying a generalized estimating equation model. Changes in disease activity during gestation and in the first year postpartum were assessed in a linear mixed model for repeated measures.Results: Thirty-one women (49 pregnancies) with persisting JIA and at least one conception were enrolled. Adjusted DAS28-CRP levels remained stable from preconception through the first trimester, but increased significantly in the second and decreased not significantly in the third. In the postpartum, adjusted disease activity peaked at 3 months after delivery, stabilized at 6 months to decrease at 1 year, although not significantly. Preconceptional DAS28-CRP and number of biological drugs predicted disease activity fluctuation during gestation. The number of biological drugs and the length of gestational exposure to biologics significantly predicted pregnancy morbidity. In particular, JIA women had a higher probability of preterm delivery compared with healthy and disease controls. Adjusted for breastfeeding and DAS28-CRP score in the third trimester, postconceptional exposure to biologics was inversely related with disease activity in the postpartum: the longer the patient continued treatment, the lower the probability of experiencing an adverse pregnancy outcome.Conclusion: These data offer novel insights on how treatment affects disease activity during pregnancy and postpartum as well as obstetric outcomes in women with JIA