Comparison of Euroregions on the Czech-Slovak Territory in the Context of EU Cross-border Cooperation

Import 02/11/2016Hlavním cílem předkládané práce je porovnání euroregionů na česko-slovenském území. Práce je uvedena teoretickými východisky přeshraniční spolupráce v Evropské unii. Definuje regionální politiku EU a v jejím rámci přeshraniční spolupráci. Další kapitola se zaměřuje na charakteristiku euroregionů se zaměřením na euroregiony na česko-slovenské hranici. Poslední obsahová kapitola je věnována analýze a komparaci vybraných euroregionů, pomocí socio-ekonomických ukazatelů a činností euroregionů v rámci projektových aktivit.The primary aim of the thesis is a comparison of Euroregions on the Czech-Slovak territory. Thesis starts with theoretical basis for cross-border cooperation in the European Union. Defines EU Regional Policy and within cross-border cooperation. Next chapter focuses on the characteristics of Euroregions with a focus on Euroregions on the Czech-Slovak border. The last chapter is dedicates the contect of the analysis and comparison of selected Euroregions with socio-economic indicators and activites within the project activities of the Euroregions.120 - Katedra evropské integracevelmi dobř

Myomedin replicas of gp120 V3 loop glycan epitopes recognized by PGT121 and PGT126 antibodies as non-cognate antigens for stimulation of HIV-1 broadly neutralizing antibodies

IntroductionImprinting broadly neutralizing antibody (bNAb) paratopes by shape complementary protein mimotopes represents a potential alternative for developing vaccine immunogens. This approach, designated as a Non-Cognate Ligand Strategy (NCLS), has recently been used for the identification of protein variants mimicking CD4 binding region epitope or membrane proximal external region (MPER) epitope of HIV-1 envelope (Env) glycoprotein. However, the potential of small binding proteins to mimic viral glycan-containing epitopes has not yet been verified.MethodsIn this work, we employed a highly complex combinatorial Myomedin scaffold library to identify variants recognizing paratopes of super candidate bNAbs, PGT121 and PGT126, specific for HIV-1 V3 loop epitopes.ResultsIn the collection of Myomedins called MLD variants targeted to PGT121, three candidates competed with gp120 for binding to this bNAb in ELISA, thus suggesting an overlapping binding site and epitope-mimicking potential. Myomedins targeted to PGT126 designated MLB also provided variants that competed with gp120. Immunization of mice with MLB or MLD binders resulted in the production of anti-gp120 and -Env serum antibodies. Mouse hyper-immune sera elicited with MLB036, MLB041, MLB049, and MLD108 moderately neutralized 8-to-10 of 22 tested HIV-1-pseudotyped viruses of A, B, and C clades in vitro.DiscussionOur data demonstrate that Myomedin-derived variants can mimic particular V3 glycan epitopes of prominent anti-HIV-1 bNAbs, ascertain the potential of particular glycans controlling neutralizing sensitivity of individual HIV-1 pseudoviruses, and represent promising prophylactic candidates for HIV-1 vaccine development

The influence of longterm unemployment on work motivation

My bachelor work consists of three parts: introduction, main part and enclosure. The introduction shows the aim and methodology of my work. The main part divides into respective elaboration, evaluation, conclusion and a list of source material. The respective elaboration has four chapters -- Motivation, Work motivation, Unemployment and Long term unemployment and these topics are defined, described and individualized. In the second part of the main part there is the evaluation. Two hundred respondents were given a questionnaire by means of Employment office in Klatovy. Their answers were evaluated and elaborated into tables and diagrams and quantified. The list of source material, publications and web-based material is alphabetically listed. In the enclosure part there is the questionnaire, used in the research and filled by the target group -- short time and long time unemployed people registered by the Employment office in Klatovy

The influence of longterm unemployment on work motivation

My bachelor work consists of three parts: introduction, main part and enclosure. The introduction shows the aim and methodology of my work. The main part divides into respective elaboration, evaluation, conclusion and a list of source material. The respective elaboration has four chapters -- Motivation, Work motivation, Unemployment and Long term unemployment and these topics are defined, described and individualized. In the second part of the main part there is the evaluation. Two hundred respondents were given a questionnaire by means of Employment office in Klatovy. Their answers were evaluated and elaborated into tables and diagrams and quantified. The list of source material, publications and web-based material is alphabetically listed. In the enclosure part there is the questionnaire, used in the research and filled by the target group -- short time and long time unemployed people registered by the Employment office in Klatovy

Possibilities of qualitative modeling in bankruptcy predictions

The issue of bankruptcy has long been paid a considerable interest at practical and theoretical levels using more sophisticated tools such as genetic algorithms, neural networks, statistical methods etc. This article describes application of less a used method of Qualitative models which can forecast changes in trends and thus contribute to solving the selected problem even without entirely accurate knowledge of the quantitative data. The model is based on three qualitative values only: increasing, constant, decreasing used to describe the corresponding trends. The result presents eleven possible scenarios and sixteen transitions among them

DataSheet_1_Myomedin replicas of gp120 V3 loop glycan epitopes recognized by PGT121 and PGT126 antibodies as non-cognate antigens for stimulation of HIV-1 broadly neutralizing antibodies.docx

IntroductionImprinting broadly neutralizing antibody (bNAb) paratopes by shape complementary protein mimotopes represents a potential alternative for developing vaccine immunogens. This approach, designated as a Non-Cognate Ligand Strategy (NCLS), has recently been used for the identification of protein variants mimicking CD4 binding region epitope or membrane proximal external region (MPER) epitope of HIV-1 envelope (Env) glycoprotein. However, the potential of small binding proteins to mimic viral glycan-containing epitopes has not yet been verified.MethodsIn this work, we employed a highly complex combinatorial Myomedin scaffold library to identify variants recognizing paratopes of super candidate bNAbs, PGT121 and PGT126, specific for HIV-1 V3 loop epitopes.ResultsIn the collection of Myomedins called MLD variants targeted to PGT121, three candidates competed with gp120 for binding to this bNAb in ELISA, thus suggesting an overlapping binding site and epitope-mimicking potential. Myomedins targeted to PGT126 designated MLB also provided variants that competed with gp120. Immunization of mice with MLB or MLD binders resulted in the production of anti-gp120 and -Env serum antibodies. Mouse hyper-immune sera elicited with MLB036, MLB041, MLB049, and MLD108 moderately neutralized 8-to-10 of 22 tested HIV-1-pseudotyped viruses of A, B, and C clades in vitro.DiscussionOur data demonstrate that Myomedin-derived variants can mimic particular V3 glycan epitopes of prominent anti-HIV-1 bNAbs, ascertain the potential of particular glycans controlling neutralizing sensitivity of individual HIV-1 pseudoviruses, and represent promising prophylactic candidates for HIV-1 vaccine development.</p