Graphics Intro 64kB Using OpenGL

Práce řeší tvorbu grafického intra s použitím OpenGL, jehož velikost nesmí přesáhnout 64 kB. Popsané a použité techniky zahrnují: Phongův osvětlovací model, mlhu, skybox, generování textur pomocí šumu a shadow mapping. Intro se odehrává ve velkoměstě, a jsou popsány techniky využité pro generování všech prvků města. Dále jsou popsány metody snížení velikosti aplikace a systém pro animaci kamery.This thesis deals with creating a graphics intro, which's size has to be below 64~kB. Described and used techniques includes: Phong reflection model, fog, skybox, generating textures from noise and shadow mapping. Intro is placed in a city and techniques of generating elements of the city are described. In addition, there are described methods for reducing file size and system for camera animation.

Skeletal Animation for GPUengine

Cílem této práce je studium technik používaných pro kosterní animace a následný návrh a implementace rozšíření pro kosterní animace do knihovny GPUEngine. V teoretické části jsou popsány techniky animací, kosterních animací a skinningu. Následuje rozbor existujících systémů kosterních animací. Navržené řešení se snaží o nízkou redundanci dat v paměti při vykreslování více kosterních modelů. Podle návrhu byl implementován základní systém kosterních animací. Dále byla vytvořena demonstrační aplikace ukazující jeho použití. Výsledný kosterní systém lze použít v jednoduchých 3D aplikacích a může sloužit jako základ pro další práce.This paper deals with studying skeletal animation techniques, and the subsequent design and implementation of skeletal animation extension for the GPUEngine library. The theoretical part describes the techniques of animation, skeletal animation and skinning. The following is an analysis of existing skeletal animation systems. The proposed solution seeks to reduce the data redundancy in the memory while rendering more skeletal models. According to the design a basic skeletal animation system has been implemented. Furthermore, a demonstration application has been created showing the skeletal system's use. The resulting skeletal system can be used in simple 3D applications and can serve as a basis for further works.

The behaviour of hyaluronan solutions in the presence of Hofmeister ions: A light scattering, viscometry and surface tension study

Dynamic light scattering (DLS), viscosity and surface tension (SFT) measurements were used to characterize influence of salts containing ions of Hofmeister series (Na 2 SO 4 , (NH 4 ) 2 SO 4 , NaSCN, NH 4 SCN and NaCl) on the behaviour of hyaluronan in diluted solutions at a temperature range of 15–45 °C. The results of the study showed that chaotropic and kosmotropic ions notably influenced the folding and unfolding of hyaluronan coils due to interactions between a respective ion and hydrophilic or hydrophobic patches present in the backbone of the polymer chains. This was mainly proved by viscosity and light scattering measurements. The temperature dependence of the hydrodynamic diameter of the hyaluronan coil determined by DLS demonstrated that combinations of chaotropic and kosmotropic ions in one salt (NaCl, NaSCN and (HN 4 ) 2 SO 4 ) can stabilize the size of the coil in a wide range of temperatures. Tensiometry measurements indicated that certain types of ions present in the solution caused an unfolding of the hyaluronan coils, leading to a decrease of SFT. © 2019 Elsevier LtdMinistry of Education, Youth and Sports of the Czech Republic - Program NPU I [LO1504]; TBU in Zlin [IGA/FT/2017/011, IGA/FT/2018/011, IGA/FT/2019/012

Variations of polymer porous surface structures via the time-sequenced dosing of mixed solvents

A new approach to polystyrene surface treatment via the time-sequenced dispensing of good and poor solvent mixtures on the rotating surface of treated substrate is presented in this study. It is demonstrated that the variation of the sequencing together with other variables (e.g., temperature and solvent concentration) affects the size and depth of pores evolving on the polystyrene surface. A model of the surface pore creation, associated with the viscoelastic phase separation, surface tension, and secondary flows caused by temperature variations and the rapid evaporation of the good solvent is proposed. Experimental results of profilometric, goniometric, and optical measurements show that this approach enables the simple and quick preparation of surfaces with various numbers, diameters, and depths of individual pores, which ultimately affects not only the wetting characteristics of the surfaces but also the fate of cells cultivated there. The presented method allows the easy preparation of a large number of structured substrates for effective cell cultivation and proliferation. © 2017 American Chemical Society.CZ.1.05/2.1.00/19.0409, ERDF, European Regional Development Fund; MOE, Ministry of EducationOperational Program Research and Development for Innovations - European Regional Development Fund (ERDF); national budget of the Czech Republic within the framework of the project Centre of Polymer Systems [CZ.1.05/2.1.00/03.0111]; Ministry of Education, Youth and Sports of the Czech Republic, Program NPU I [LO1504]; European Regional Development Fund [CZ.1.05/2.1.00/19.0409]; TBU [IGA/FT/2015/014, IGA/FT/2016/013]Operational Program Research and Development for Innovations - European Regional Development Fund (ERDF); national budget of the Czech Republic within the framework of the project Centre of Polymer Systems [CZ.1.05/2.1.00/03.0111]; Ministry of Education, Youth and Sports of the Czech Republic, Program NPU I [LO1504]; European Regional Development Fund [CZ.1.05/2.1.00/19.0409]; TBU [IGA/FT/2015/014, IGA/FT/2016/013

Dual patterning of self-assembling spider silk protein nanofibrillar networks

Self-assembly of a recombinant spider silk protein into nanofibrillar networks in combination with photolithography is used to produce diversely functionalized micropattern. Amino-modified substrates coated with a positive tone photoresist are processed into 1 mu m deep arbitrarily shaped microwells, at the bottom of which spider silk proteins are covalently coupled to the deprotected aminated surface. The protein layer serves to seed the self-assembly of nanofibrils from the same protein in the microwells, forming immobilized few nanometers thin networks after the stripping of the photoresist. The nanofibrous micropattern can be functionalized by employing fluorescently modified spider silk variants during the self-assembly or by later covalent modification with nucleic acids. By repeating the photolithography and fibril assembly procedures, two functionally different and spatially defined pattern are created.Bavarian-Czech Academic Agency (Bayerisch-Tschechische Hochschulagentur) BTHA Grant [JC-2019-21]; Czech Science Foundation [22-33307S]; TBU Grant [IGA/FT/2022/009]; Projekt DEALJC‐2019‐21; Tomas Bata University in Zlin, TBU: IGA/FT/2022/009; Grantová Agentura České Republiky, GA ČR: 22–33307

The impact of polymer grafting from a graphene oxide surface on its compatibility with a PDMS matrix and the light-induced actuation of the composites

Poly(dimethyl siloxane) (PDMS)-based materials with improved photoactuation properties were prepared by the incorporation of polymer-grafted graphene oxide particles. The modification of the graphene oxide (GO) surface was achieved via a surface initiated atom transfer radical polymerization (SI ATRP) of methyl methacrylate and butyl methacrylate. The modification was confirmed by thermogravimetric analysis, infrared spectroscopy and electron microscopy. The GO surface reduction during the SI ATRP was investigated using Raman spectroscopy and conductivity measurements. Contact angle measurements, dielectric spectroscopy and dynamic mechanical analyses were used to investigate the compatibility of the GO filler with the PDMS matrix and the influence of the GO surface modification on its physical properties and the interactions with the matrix. Finally, the thermal conductivity and photoactuation properties of the PDMS matrix and composites were compared. The incorporation of GO with grafted polymer chains, especially poly(n-butyl methacrylate), into the PDMS matrix improved the compatibility of the GO filler with the matrix, increased the energy dissipation due to the improved flexibility of the PDMS chains, enhanced the damping behavior and increased the thermal conductivity. All the changes in the properties positively affected the photoactuation behavior of the PDMS composites containing polymer-grafted GO. © 2017 by the authors.LO1504, MOE, Ministry of EducationGrant Agency of the Czech Republic [16-20361Y]; Ministry of Education, Youth and Sports of the Czech Republic-program NPU I [LO1504]; SRDA [APVV-15-0545]; VEGA [VEGA 2/0161/17]; Slovak Academy of Sciences [SAS-MOST JRP 2014-9

Preparation of hierarchically structured polystyrene surfaces with superhydrophobic properties by plasma-assisted fluorination

The nanotexturing of microstructured polystyrene surfaces through CF4 plasma chemical fluorination is presented in this study. It is demonstrated that the parameters of a surface micropore-generation process, together with the setup of subsequent plasma-chemical modifications, allows for the creation of a long-term (weeks) surface-stable micro- and nanotexture with high hydrophobicity (water contact angle >150 degrees). Surface micropores were generated initially via the time-sequenced dosing of mixed solvents onto a polystyrene surface (Petri dish) in a spin-coater. In the second step, tetrafluoromethane (CF4) plasma fluorination was used for the generation of a specific surface nanotexture and the modulation of the surface chemical composition. Experimental results of microscopic, goniometric, and spectroscopic measurements have shown that a single combination of phase separation methods and plasma processes enables the facile preparation of a wide spectrum of hierarchically structured surfaces differing in their wetting properties and application potentials.Ministry of Education, Youth and Sports of the Czech Republic-Program NPU I [LO1504]; European Regional Development Fund [CZ.1.05/2.1.00/19.0409]; TBU [IGA/FT/2017/011, IGA/FT/2018/011, IGA/FT/2019/012

Electrospinning of hyaluronan using polymer coelectrospinning and intermediate solvent

In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight (Mw), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA Mw on HA/PEO nanofiber morphology, were examined, respectively. © 2019 by the authors

Preparation of textured surfaces on aluminum-alloy substrates

The ways of producing porous-like textured surfaces with chemical etching on aluminum-alloy substrates were studied. The most appropriate etchants, their combination, temperature, and etching time period were explored. The influence of a specifically textured surface on adhesive joints' strength or superhydrophobic properties was evaluated. The samples were examined with scanning electron microscopy, profilometry, atomic force microscopy, goniometry, and tensile testing. It was found that, with the multistep etching process, the substrate can be effectively modified and textured to the same morphology, regardless of the initial surface roughness. By selecting proper etchants and their sequence one can prepare new types of highly adhesive or even superhydrophobic surfaces. © 2018 by the authors.Ministry of Education, Youth, and Sports of the Czech Republic-Program NPU I [LO1504]; European Regional Development Fund [CZ.1.05/2.1.00/19.0409]; TBU grant [IGA/FT/2017/011, IGA/FT/2018/011

Whole-Cell Protein Profiles of Disintegrated Freshwater Green Algae and Cyanobacterium

The influence of cultivation methods and postharvesting treatment on protein profiles of green freshwater microalgae Chlorella kessleri, Scenedesmus quadricauda, and Chlorella sp. and cyanobacterium Spirulina platensis were evaluated. The comparison of protein profiles in algal biomass originated from the autotrophic cultivation in an outdoor open circulating cascade-type cultivation apparatus in thin-layer, a solar photobioreactor, and from the heterotrophic cultivation regime in a fermenter. All tested algae contained protein bands in the area between 14.3-27 and 70-116 kDa. Protein profiles revealed much higher heterogenity in the area between 30-70 kDa. © 2016 Taylor & Francis.projects of the internal grants of Tomas Bata University in Zlin [IGA/22/FT/11/D, IGA/FT/2012/038/D