Pearl Millet (Pennisetum glaucum [L. R. Rr.]) Varietal Loss and its Potential Impact on Smallholder Farmers in Northern Nigeria: A Review

Pearl millet is an alternative source of livelihood for poor farmers who use it to supplement food and income. Pearl millet has high medicinal value and its consumption reduce the risk of type 2 diabetes, constipation and anemia. Pearl millets is an excellent source of protein and fiber, and a great source of starch, making it a high-energy food. It is an exceptional food source for babies from six months of age because it has high nutrients and is easily digestible. Pearl millet is the most widely cultivated millet varieties in the world. The cereal crop is grown in arid, infertile, marginal lands in Africa and Asia, and cope with unpredictable climatic conditions. Nigeria is the second largest producer of pearl millet in the world with tremendous amount of pearl millet diversity. However, there is growing recognition that the diversity of pearl millet in northern Nigeria is vastly diminishing thereby affecting the livelihood of smallholder farmers. This paper reviews the erosion of pearl millet, multiple drivers of loss of local varieties of pearl millet, and presents some of the strategies that can be implemented to save the erosion of its diversity in northern Nigeria

Potato and Bambara groundnut ammonium transporter (AMT1) structure and variation in expression level in potato leaf tissue in response to nitrogen form and availability

Thesis (MTech (Agriculture))--Cape Peninsula University of Technology, 2014.Plants require nitrogen (N) to support desired production levels. Nitrogen fertilization strategy is a major consideration in field management with regard to achieving both economic and environmental objectives. For instance, in potato, insufficient N supply reduces tuber size and overall yield while excessive N supply can reduce tuber quality and increase environmental risk through nitrate (NO3-) leaching and nitrous oxide emission. Selection of an adequate N fertilizer application rate for crops is difficult, due to marked variations in soil N supply and crop N demand in both the field and over time. This research was conducted to characterise the ammonium transporter gene (AMT1) of Bambara groundnut and potato using molecular biology and bioinformatics methods. Nucleotide database sequences were used to design AMT1-specific primers which were used to amplify and sequence the core-region of the gene from Bambara groundnut and potato. Bioinformatics techniques were used to predict the structure and infer properties of the proteins. Nucleotide sequence alignment and phylogenetic analysis indicate that BgAMT1 and PoAMT1 are indeed from the AMT1 family, due to the clade and high similarity they respectively shared with other plant AMT1 genes. Amino acid sequence alignment showed that BgAMT1 is 92%, 89% and 87% similar to PvAMT1.1, GlycineAMT1 and LjAMT1.1 respectively, while PoAMT1 is 92%, 83% and 76% similar to LeAMT1.1, LjAMT1.1 and LeAMT1.2 respectively. BgAMT1 and PoAMT1 fragments were shown to correspond to the 5th - 10th transmembrane spanning-domains. Mutation of Bg W1A-L and S28A (for BgAMT1) and Po S70A (for PoAMT1) is predicted to enhance ammonium (NH4+) transport activity. Residues Bg D23 (for BgAMT1) and Po D16 (for PoAMT1) must be preserved otherwise NH4+ transport activity is inhibited. In all, BgAMT1 and PoAMT1 play a role in N uptake from the root while BgAMT1 may contribute more in different steps of rhizobia interaction. In an investigation of the correlation between AMT1 gene expression levels and leaf chlorophyll content index (CCI) with plant N status, potato plants were grown in a hydroponic greenhouse with 0.75 or 7.5 mM NO3- and 0.75 or 7.5 mM NH4+ as forms of N supply in a completely randomized design. Leaf CCI as measured by chlorophyll content meter, showed that an increase in N supply results in increased leaf CCI in response to both forms of N. Total RNA was isolated from leaf sampled at 28 days after treatment and expression level of the AMT1 gene was determined by reverse transcription-qPCR using a second set of primers designed for qPCR. The results showed that expression levels of AMT1 increased from 8.731 ± 2.606 when NO3- supply was high to 24.655 ± 2.93 when NO3- supply was low. However, there was no significant response in AMT1 expression levels to changes in NH4+. This result suggested that AMT1 transports NO3- less efficiently than NH4+, and thus more transport channels are required in the cell membrane when NO3- levels are low. Such variation in AMT1 expression levels are not necessary for NH4+ transport since the transport mechanism for NH4+ is efficient even at low NH4+ levels

Performance of fusarium oxysporum EKT01/02 isolate in cyanide biodegradation system

This study reports a cyanide resistant and/or tolerant fungus, isolated from the rhizosphere of Zea mays contaminated with cyanide-based pesticides. The isolate was characterised using molecular biology. The effect of free cyanide and heavy metals on the growth of isolate in a synthetic gold mine wastewater was examined. The molecular analyses identified the isolate as Fusarium oxysporum EKT01/02 (KU985430/KU985431). The isolate had a free cyanide degradation efficiency of 77.6%. The results indicated greater growth impairment in culture containing Arsenic (optical density 1.28 and 1.458) and cyanide (optical density 1.315 and 1.385). Higher growth was observed in all cultures supplemented with extracellular polymeric substance. This study showed that the isolate possesses wide substrate utilisation mechanism that could be deployed in environmental engineering applications

Assessment of Turkey Farming Management Practices by Small-Scale Rural Farmers in Eastern Nigeria

A survey of rural turkey farmers was conducted in three villages in the Eastern part of Nigeria, namely: Amukwa, Amaeze and Nguru. The aim of the study was to assess the prevailing management and production practices of turkey farmers in the area. Information obtained included flock size, reasons for keeping birds, breeding and management practices among others. The multistage random sampling method was used to collect information from 180 respondents. Results obtained revealed variability in response among flock sizes (1–20), with most (90%) respondents being part-time farmers. Mean scores, in terms of the level of adoption of standard management practices by turkey farmers, were given as cleaning and disinfection of pen before restocking ( = 4.60), removal of droppings ( = 4.57), and changing and replacement of litters ( = 4.4). Beak trimming ( = 1.27) was the lowestranked farm practice by farmers. It was concluded that the management practices and production of turkey in the study area are below standard when compared to those in developed societies. Adequate support for farmers in terms of finance and regular training, among others, by relevant agencies including the government may help improve the production of turkey in the area