Bending Family Friendly into Fear: Nostalgia, Minstrelsy and Horror in Bendy and the Ink Machine

When one thinks of Disney’s Mickey Mouse, fear and horror are not terms normally associated with this iconic American cartoon character; however, the video game Bendy and the Ink Machine turns animated bodies (cartoons) into bodies the player fears. In this game family friendly cartoon characters are transformed into figures of fear. Furthermore, Bendy and the Ink Machine does this by making the bending of Black bodies visible through what I call the gameic gaze. The transformation from family friendly into fear happens through the resistive gaze, the gameic gaze, which lingers on the bending of the diegetic cartoons. Bendy and the Ink Machine actualizes the historical contorting of the Black body starting in slavery and continued by the entertainment industry through bending. Bending and the Ink Machine makes the minstrel origins of cartoons visible through the gameic gaze

ACVIM consensus statement guidelines for the diagnosis, classification, treatment, and monitoring of pulmonary hypertension in dogs.

Pulmonary hypertension (PH), defined by increased pressure within the pulmonary vasculature, is a hemodynamic and pathophysiologic state present in a wide variety of cardiovascular, respiratory, and systemic diseases. The purpose of this consensus statement is to provide a multidisciplinary approach to guidelines for the diagnosis, classification, treatment, and monitoring of PH in dogs. Comprehensive evaluation including consideration of signalment, clinical signs, echocardiographic parameters, and results of other diagnostic tests supports the diagnosis of PH and allows identification of associated underlying conditions. Dogs with PH can be classified into the following 6 groups: group 1, pulmonary arterial hypertension; group 2, left heart disease; group 3, respiratory disease/hypoxia; group 4, pulmonary emboli/pulmonary thrombi/pulmonary thromboemboli; group 5, parasitic disease (Dirofilaria and Angiostrongylus); and group 6, disorders that are multifactorial or with unclear mechanisms. The approach to treatment of PH focuses on strategies to decrease the risk of progression, complications, or both, recommendations to target underlying diseases or factors contributing to PH, and PH-specific treatments. Dogs with PH should be monitored for improvement, static condition, or progression, and any identified underlying disorder should be addressed and monitored simultaneously

An Extremal Problem for Characteristic Functions

Suppose E is a subset of the unit circle T and Hinfinity C Linfinity is the Hardy subalgebra. We examine the problem of finding the distance from the characteristic function of E to znHinfinity. This admits an alternate description as a dual extremal problem. Precise solutions are given in several important cases. The techniques used involve the theory of Toeplitz and Hankel operators as well as the construction of certain conformal mappings

BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5′-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized the derivatized surfaces for the chemical attachment of oligonucleotides and evaluate the properties relevant for the amplification process: surface density, thermal stability towards thermocycling, functionalization reproducibility and storage stability. The derivatization process, first developed for glass slides, was then adapted to microfabricated glass channels containing integrated fluidic connections. This implementation resulted in an important reduction of reaction times, consumption of reagents and process automation. Innovative analytical methods for the characterization of attached DNA were developed for assessing the surface immobilized DNA content after amplification. The results obtained showed that the BTA chemistry is compatible and suitable for forming highly dense arrays of DNA colonies with optimal surface coverage of about 10 million colonies/cm2 from the amplification of initial single-template DNA molecules immobilized. We also demonstrate that the dsDNA colonies generated can be quantitatively processed in situ by restriction enzymes digestion. DNA colonies generated using the BTA reagent can be used for further sequence analysis in an unprecedented parallel fashion for low-cost genomic studie