Effects of Ovarian Hormones on the Growth and the Differentiation of the Mouse Blastocyst During Delayed Implantation

Effects of progesterone and estradiol-17β on the growth and the differentiation of the mouse blastocyst during the pre-implantation and implantation stages was investigated inducing to delayed implantation by ovariectomy. When the ovariectomy was followed by daily injection of 1 mg progesterone (Group II), the area of blastocyst on the 6th day of pregnancy was significantly larger than that on the 4th day of pregnancy. When a single dose of estradiol-17β (0.5μg) was injected to ovariectomized and progesterone treated mice on the 4th or 6th day of pregnancy (Group III-A and III-B), the implantation was observed between 24 and 30 hrs. after the injection. In addition the area of blastocyst in Group III-A was significantly larger than that in Group II after 24 hrs. estradiol-17β injection and was as large as those in intact group (Group I) and Group II after 6 hrs. estradiol-1719 injection. The area of blastocyst in Group III-B was also significantly larger than that in Group II on and after 18 hrs. estradiol-17β injection. These results indicate that progesterone and estradiol-17β are important for the growth and the differentiation of delayed-implanting mouse blastocyst and they induce the implantation.Article信州大学農学部紀要 18(2): 133-144(1981)departmental bulletin pape

Involvement of a cell adhesion molecule, TSLC1, in human oncogenesis

[Curriculum Vitae & Summary] International Symposium on Tumor Biology in Kanazawa 2004 / Kanazawa, Japan February 12 and 13, 200

High-temperature thermoelectric properties of the double-perovskite ruthenium oxide (Sr1−x_{1-x}Lax_x)2_2ErRuO6_6

We have prepared polycrystalline samples of (Sr$_{1-x}$La$_x$)$_2$ErRuO$_6$ and (Sr$_{1-x}$La$_x$)$_2$YRuO$_6$, and have measured the resistivity, Seebeck coefficient, thermal conductivity, susceptibility and x-ray absorption in order to evaluate the electronic states and thermoelectric properties of the doped double-perovskite ruthenates. We have observed a large Seebeck coefficient of -160 $\mu$V/K and a low thermal conductivity of 7 mW/cmK for $x$=0.1 at 800 K in air. These two values are suitable for efficient oxide thermoelectrics, although the resistivity is still as high as 1 $\Omega$cm. From the susceptibility and x-ray absorption measurements, we find that the doped electrons exist as Ru$^{4+}$ in the low spin state. On the basis of the measured results, the electronic states and the conduction mechanism are discussed.Comment: 6 pages, 4 figures, J. Appl. Phys. (accepted

Tumor suppressor in lung cancer 1 (TSLC1) alters tumorigenic growth properties and gene expression

BACKGROUND: Introduction of cDNA or genomic clones of the tumor suppressor in lung cancer 1 (TSLC1) gene into the non-small cell lung cancer line, A549, reverses tumorigenic growth properties of these cells. These results and the observation that TSLC1 is down-regulated in a number of tumors suggest that TSLC1 functions as a critical switch mediating repression of tumorigenesis. RESULTS: To investigate this mechanism, we compared growth properties of A549 with the TSLC1-containing derivative. We found a G1/S phase transition delay in 12.2. Subtractive hybridization, quantitative PCR, and TranSignal Protein/DNA arrays were used to identify genes whose expression changed when TSLC1 was up-regulated. Members of common G1/S phase regulatory pathways such as TP53, MYC, RB1 and HRAS were not differentially expressed, indicating that TSLC1 may function through an alternative pathway(s). A number of genes involved in cell proliferation and tumorigenesis were differentially expressed, notably genes in the Ras-induced senescence pathway. We examined expression of several of these key genes in human tumors and normal lung tissue, and found similar changes in expression, validating the physiological relevance of the A549 and 12.2 cell lines. CONCLUSION: Gene expression and cell cycle differences provide insights into potential downstream pathways of TSLC1 that mediate the suppression of tumor properties in A549 cells

Enhanced Photoelectrocatalytic Activity of FTO/WO3/BiVO4 Electrode by Modified With Gold Nanoparticles for Water Oxidation Under Visible Light Irradiation

Gold nanoparticles were successfully deposited on FTO/WO3/BiVO4 electrode surface by means of electrolysis of AuCl4 - ions. The composite films were characterized by SEM, XPS and XRD techniques. An increase in photocurrent and a negative shift of onset potential for water oxidation were observed upon modification of the electrode surface with the Au particles. The electrochemical impedance spectroscopy was used to confirm the acceleration of charge transfer process by Au deposition at the electrode surface. The photocurrent action spectrum did not correlate with the plasmonic absorbance of Au nanoparticles at 560 nm, suggesting that the Au nanoparticles increased charge separation without undergoing a plasmon resonance effect under visible light irradiation

Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells

AbstractWe show here novel intracellular Ca2+ oscillation in ν-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca2+ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca2+ oscillation is negatively regulated by PKC, which modulates Ca2+ influx in ν-K-ras-transformed NIH3T3 cells

ダイイッカイ タンキ シュウチュウ コウカイ ケンキュウカイ ホウコク ダイガク ケイエイ ジンザイ ノ ヨウセイ オ メザシテ ダイガク ケイエイ ジンザイ ノ ヨウセイ オ メザシテ

ご紹介いただきましたように昭和38年大学職員になりました。それまでの大学の職員は内輪で採っていた時代でしたが、その前の年から一般に公開採用された第2回目です。私は、大東亜戦争が始まる前の年、1940年生まれです。ですから ..

Compensatory Upregulation of Myelin Protein Zero-Like 2 Expression in Spermatogenic Cells in Cell Adhesion Molecule-1-Deficient Mice

The cell adhesion molecule-1 (Cadm1) is a member of the immunoglobulin superfamily. In the mouse testis, Cadm1 is expressed in the earlier spermatogenic cells up to early pachytene spermatocytes and also in elongated spermatids, but not in Sertoli cells. Cadm1-deficient mice have male infertility due to defective spermatogenesis, in which detachment of spermatids is prominent while spermatocytes appear intact. To elucidate the molecular mechanisms of the impaired spermatogenesis caused by Cadm1 deficiency, we performed DNA microarray analysis of global gene expression in the testis compared between Cadm1-deficient and wild-type mice. Out of the 25 genes upregulated in Cadm1-deficient mice, we took a special interest in myelin protein zero-like 2 (Mpzl2), another cell adhesion molecule of the immunoglobulin superfamily. The levels of Mpzl2 mRNA increased by 20-fold and those of Mpzl2 protein increased by 2-fold in the testis of Cadm1-deficient mice, as analyzed with quantitative PCR and western blotting, respectively. In situ hybridization and immunohistochemistry demonstrated that Mpzl2 mRNA and protein are localized in the earlier spermatogenic cells but not in elongated spermatids or Sertoli cells, in both wild-type and Cadm1-deficient mice. These results suggested that Mpzl2 can compensate for the deficiency of Cadm1 in the earlier spermatogenic cells