4-[3,5-Bis(ethoxy­carbon­yl)-2,6-dimethyl-4-pyrid­yl]pyridinium nitrate

In the title mol­ecular salt, C18H21N2O4 +·NO3 −, the dihedral angle between the two pyridine rings is 61.24 (8)°. In the crystal, the cation and anion are linked by inter­molecular N—H⋯O hydrogen bonds

Diethyl 2,6-dimethyl-4-(4-pyrid­yl)-1,4-dihydro­pyridine-3,5-dicarboxyl­ate

In the title compound, C18H22N2O4, the dihedral angle between the two rings is 87.90 (6)°. The mean devation of the atoms in the dihydropyridine plane is 0.082 (3) Å. In the crystal, mol­ecules are linked by inter­molecular N—H⋯N hydrogen bonds, generating chains

Detection of Microcystin-Producing Cyanobacteria in a Reservoir by Whole Cell Quantitative PCR

AbstractCyanobacterial blooms are of increasing concern due to their negative impacts on environment and cyanobacteria-producing cyanotoxins, which create serious threats to animal and human health. Early detection of the harmful cyanotoxin-producing cyanobacteria is vital for the water management. In this study, we compared the amplification specificity for microcystin-producing cyanobacteria with primers targeting 16S rRNA genes, phycocyanin operon, and regions of mcy gene clusters. To detect presence of microcystin-producing cyanobacteria in a drinking water sourced reservoir, whole cell PCR assay was performed to amplify partial mcyA, mcyE, and the results were compared with that of direct microscopic counts based on morphologic identification. The positive liner correlation of the Microcystis colony by microscopic counts with mcyA containing cell, which was quantified by whole cell quantitative RT-PCR assay, was further confirmed. The results indicated that the microcystin-producer in the reservoir was mainly Microcystis. Therefore, we provided a simple, rapid, sensitive and applicable method for early detection of toxic cyanobacteria

Financial Comprehensive Appraisal Empirical Research on Grey Relational Model ——Testing in Listed Companies in Real Estate Industry in China

The financial comprehensive appraisal method plays an essential role in decision-making for investors and reflecting real company’s financial comprehensive situation. Because of the grey characteristics of financial information, this paper constructs a grey relational model with a index system of financial comprehensive appraisal by grey relation theory. It is applied to listed companies in real estate industry in China and tested in the both dynamic and static state, and then the result is that the ranking of financial comprehensive evaluation of the listed companies in the real estate industry is almost as same as the real ranking. It shows that the financial comprehensive appraisal method on the grey relational analysis is a kind of effective and good method of evaluating company’s financial comprehensive situation