Isolation of Caffeine from Carbonated Beverages

The work presented on the isolation of naturally occurring alkaloid from carbonated beverages. The extensive presence of caffeine in different plants plays an important role in the long-standing acceptance of caffeine-containing products. Caffeine (3,7-dihydro-1, 3,7-trimethyl-1H-purine-2,6-dione or 1,3,7-trimethylxanthine) is an alkaloid belongs to Methylxanthine family. Liquid-liquid extraction methods were used in the assay of research work. Chloroform was taken as extracting solvent. Solid residue of caffeine was recrystallized from 95% ethanol using 5ml/gram (5ml per gram). It is declared to raise caffeine, effects a number of different drugs include Paracetamol, Benzodiazepines and Aspirin and amount of plasma free Fatty acids increases. While inform that in regular sleeping interaction caffeine take place and raise the absorption of certain drugs. Changes in drug metabolizing enzymes, acts as an agent in a microsomal system of the body. The highest amount of caffeine dry crystal is extracted in sting sample while the 7up sample is free from caffeine

Evaluation and toxicological quantification of undeclared allopathics and adulterated synthetic steroids in herbal antihypertensive preparations

Purpose: To evaluate raw and finished dosage form of herbal antihypertensives for quantification of undeclared allopathic contents and synthetic steroids adulteration in each unit and in total daily dose.Methods: Analysis of herbal products for allopathic drugs adulteration was carried out using HPLC techniques. The methods were reproduced with optimized extraction and chromatographic conditions. Calibration curves were reconstructed for validation purposes.Results: The herbal products were adulterated with various synthetic drugs. The concentrations (mean ± SD) were: atenolol (50.06 ± 1.20 mg/unit dose),  propranolol (20.30 ± 0.44 mg/unit dose, 28.26 ± 0.06 mg/unit dose, 15.40 ± 1.58 mg/unit dose), ACE inhibitors i.e. captopril (52.99 ± 0.49 mg/unit dose) and frusemide (42.02 ± 0.88 mg/unit dose). For the synthetic steroids, the levels (mean ± SD) were prednisolone (13.67 ± 0.50 mg/unit dose), methyl prednisolone (4.18 ± 0.02 mg/unit dose), betamethasone (0.56 ± 0.06 mg/unit dose) and dexamethasone (1.75 ± 0.11 mg/unit dose).Conclusion: Administration of adulterated remedies can cause severe toxicity and is a serious safety concerns for public health. Therefore, to maximize consumer safety, appropriate rules and regulations should be developed for registration of herbal remedies.Keywords: Herbal medicines, Adulterants, Allopathic drugs, Synthetic steroid

Urease Inhibitor Application Stages and Nitrogen Levels Influenced on Morpo-Phenological Traits of Wheat Cultivars

A field trial was carried out at New Developmental Farm of The University Agriculture, Peshawar, Pakistan during winter 2012-13, in order to study the urease inhibitor application stages and nitrogen levels influenced on morpo-phenological traits of wheat cultivars. Therefore the field experiment was conducted in randomized complete block design with split plot arrangement having four replications.  Nitrogen levels (60, 120 and 150 kg ha-1) and urease inhibitor  stages (100% sowing stage, 50% sowing stage + 50% booting stage and 100% booting stage) were allotted to main plots, while  wheat cultivars (Siran and Atta Habib)  were allotted to sub plots.  Plots treated with 120 kg N ha-1 took maximum days to booting (128), improved plant height (97.9 cm), leaf area tiller-1 (117.8 cm2), spike length (11.3 cm) and biological yield (10382 kg ha-1) but maximum (185) days to maturity  was observed when plots treated with 150 kg N ha-1 as compared with control plots. Application of urease inhibitor 100% at sowing stage took maximum booting (133) days, maturity (186) days, improved plant height (102 cm), leaf area tiller-1 (128 cm2), spike length (11.6 cm) and biological yield (11386 kg ha-1) as compared with urease application 100% at booting stage. Wheat cultivar Siran had significantly took maximum booting (123) days, maturity (178) days, plant height (94.5 cm), leaf area tiller-1 (97.6 cm2), spike length (10.3 cm) and biological yield (9331 kg ha-1) as compared to Atta Habib. Hence cultivar Siran treated with 120 kg N ha-1 and coated urease inhibitor 100% at sowing stage produced the best results in terms of plant height, leaf area tiller-1, physiological maturity and biological yield. Keywords: Wheat (Triticum aestivum L.), urease inhibitor application stages, nitrogen levels, wheat cultivars, phenology, morpholog