Assessment of the tissue distribution of transplanted human endothelial progenitor cells by radioactive labeling

Background— Transplantation of endothelial progenitor cells (EPCs) improves vascularization and left ventricular function after experimental myocardial ischemia. However, tissue distribution of transplanted EPCs has not yet been monitored in living animals. Therefore, we tested whether radioactive labeling allows us to detect injected EPCs

Cutting simulation with consideration of the material hardening in the Shear Zone of AISI1045

By the use of high energy synchrotron X-ray diffraction it was possible to determine the stress state in the chip formation zone during orthogonal cutting of AISI1045. The analysis of the diffractograms showed a hardening of the material during the movement through the shear zone. For this reason nano indentation experiments on prepared chips have been carried out. With these experiments, the material hardening has been confirmed. The nano indentation experiments were reproduced by FEM simulations and it was possible to determine flow curves of the hardened material above the shear zone based on existing flow curves of AISI1045. Thus, cutting simulations have been carried out, which considered the material hardening in the shear zone. The simulation results were then compared with the results of the in-situ strain measurements

J004457+4123 (Sharov 21): not a remarkable nova in M31 but a background quasar with a spectacular UV flare

We announce the discovery of a quasar behind the disk of M31, which was previously classified as a remarkable nova in our neighbour galaxy. The paper is primarily aimed at the outburst of J004457+4123 (Sharov 21), with the first part focussed on the optical spectroscopy and the improvement in the photometric database. Both the optical spectrum and the broad band spectral energy distribution of Sharov 21 are shown to be very similar to that of normal, radio-quiet type 1 quasars. We present photometric data covering more than a century and resulting in a long-term light curve that is densely sampled over the past five decades. The variability of the quasar is characterized by a ground state with typical fluctuation amplitudes of ~0.2 mag around B~20.5, superimposed by a singular flare of ~2 yr duration (observer frame) with the maximum at 1992.81 where the UV flux has increased by a factor of ~20. The total energy in the flare is at least three orders of magnitudes higher than the radiated energy of the most luminous supernovae, provided that it comes from an intrinsic process and the energy is radiated isotropically. The profile of the flare light curve appears to be in agreement with the standard predictions for a stellar tidal disruption event where a ~10 M_sun giant star was shredded in the tidal field of a ~2...5 10^8 M_sun black hole. The short fallback time derived from the light curve requires an ultra-close encounter where the pericentre of the stellar orbit is deep within the tidal disruption radius. Gravitational microlensing provides an alternative explanation, though the probability of such a high amplification event is very low.Comment: Accepted for publication in Astronomy and Astrophysics, 14 pages, 11 figure

Die Auswirkungen von Stickstoffmangel auf die Genomevolution in Escherichia coli

Bakterien nehmen Nährstoffe aus ihrer Umgebung auf und nutzen diese für die de novo Synthese von Aminosäuren, Nukleotiden und anderen Biomolekülen. Stickstoff, ein wesentlicher Bestandteil von Biomolekülen, ist oft ein limitierender Faktor für das Wachstum in Ökosystemen. Die vorliegende Studie zeigt, dass Proteine, die in die Stickstoff-Stressantwort von Escherichia coli involviert sind,eine reduzierte Anzahl an stickstoffreichen Aminosäuren aufweisen. Zudem führte andauernder Stickstoffmangel in kontinuierlichen E. coli Kulturen zu weiteren Reduktionen des Stickstoffbedarfs von Proteinen durch Änderungen der Genexpression. Die Adaption an den persistenten Stickstoffmangel erhöhte außerdem die Fitness der Bakterien unter diesen Nährstoffbedingungen. Die vorliegenden Daten sind von grosser Bedeutung für die Quantfizierung der Stärke des durch Stickstoffmangel ausgelösten Selektionsdruckes auf die molekulare Evolution von E. coli

A phenomenological model for the X-ray spectrum of Nova V2491 Cygni

The X-ray flux of Nova V2491 Cyg reached a maximum some forty days after optical maximum. The X-ray spectrum at that time, obtained with the RGS of XMM-Newton, shows deep, blue-shifted absorption by ions of a wide range of ionization. We show that the deep absorption lines of the X-ray spectrum at maximum, and nine days later, are well described by the following phenomenological model with emission from a central blackbody and from a collisionally ionized plasma (CIE). The blackbody spectrum (BB) is absorbed by three main highly-ionized expanding shells; the CIE and BB are absorbed by cold circumstellar and interstellar matter that includes dust. The outflow density does not decrease monotonically with distance. The abundances of the shells indicate that they were ejected from an O-Ne white dwarf. We show that the variations on time scales of hours in the X-ray spectrum are caused by a combination of variation in the central source and in the column density of the ionized shells. Our phenomenological model gives the best description so far of the supersoft X-ray spectrum of nova V2491 Cyg, but underpredicts, by a large factor, the optical and ultraviolet flux. The X-ray part of the spectrum must originate from a very different layer in the expanding envelope, presumably much closer to the white dwarf than the layers responsible for the optical/ultraviolet spectrum. This is confirmed by absence of any correlation between the X-ray and UV/optical observed fluxes.Comment: 11 pages, 6 figure

A remarkable recurrent nova in M 31: The predicted 2014 outburst in X-rays with Swift

The M 31 nova M31N 2008-12a was recently found to be a recurrent nova (RN) with a recurrence time of about 1 year. This is by far the fastest recurrence time scale of any known RNe. Our optical monitoring programme detected the predicted 2014 outburst of M31N 2008-12a in early October. We immediately initiated an X-ray/UV monitoring campaign with Swift to study the multiwavelength evolution of the outburst. We monitored M31N 2008-12a with daily Swift observations for 20 days after discovery, covering the entire supersoft X-ray source (SSS) phase. We detected SSS emission around day six after outburst. The SSS state lasted for approximately two weeks until about day 19. M31N 2008-12a was a bright X-ray source with a high blackbody temperature. The X-ray properties of this outburst were very similar to the 2013 eruption. Combined X-ray spectra show a fast rise and decline of the effective blackbody temperature. The short-term X-ray light curve showed strong, aperiodic variability which decreased significantly after about day 14. Overall, the X-ray properties of M31N 2008-12a are consistent with the average population properties of M 31 novae. The optical and X-ray light curves can be scaled uniformly to show similar time scales as those of the Galactic RNe U Sco or RS Oph. The SSS evolution time scales and effective temperatures are consistent with a high-mass WD. We predict the next outburst of M31N 2008-12a to occur in autumn 2015.Comment: 13 pages, 7 figures, 3 tables; accepted for publication in A&

Discovery of a new photometric sub-class of faint and fast classical novae

We present photometric and spectroscopic follow-up of a sample of extragalactic novae discovered by the Palomar 60-inch telescope during a search for "Fast Transients In Nearest Galaxies" (P60-FasTING). Designed as a fast cadence (1-day) and deep (g < 21 mag) survey, P60-FasTING was particularly sensitive to short-lived and faint optical transients. The P60-FasTING nova sample includes 10 novae in M31, 6 in M81, 3 in M82, 1 in NGC2403 and 1 in NGC891. This significantly expands the known sample of extragalactic novae beyond the Local Group, including the first discoveries in a starburst environment. Surprisingly, our photometry shows that this sample is quite inconsistent with the canonical Maximum Magnitude Rate of Decline (MMRD) relation for classical novae. Furthermore, the spectra of the P60-FasTING sample are indistinguishable from classical novae. We suggest that we have uncovered a sub-class of faint and fast classical novae in a new phase space in luminosity-timescale of optical transients. Thus, novae span two orders of magnitude in both luminosity and time. Perhaps, the MMRD, which is characterized only by the white dwarf mass, was an over-simplification. Nova physics appears to be characterized by quite a rich four-dimensional parameter space in white dwarf mass, temperature, composition and accretion rate.Comment: Submitted to ApJ, 12 pages. High resolution version at http://www.astro.caltech.edu/~mansi/msFasting.pd