Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions

The best levels of broad-spectrum Tospovirus resistance reported in tomatoes thus far are conferred by the Sw-5 locus. This locus contains at least five paralogues (denoted Sw-5a through Sw-5e), of which Sw-5b represents the actual resistance gene. Here we evaluated a panel of seven PCR primer pairs matching different sequences within a genomic region spanning the Sw-5a and Sw-5b gene cluster. Primer efficiency evaluation was done employing tomato isolines with and without the Sw-5 locus. One primer pair produced a single and co-dominant polymorphism between susceptible and resistant isolines. Sequence analysis of these amplicons indicated that they were specific for the Sw-5 locus and their differences were due to insertions/deletions. The polymorphic SCAR amplicon encompass a conserved sequence of the promoter region of the functional Sw-5b gene, being located in the position -31 from its open reading frame. This primer pair was also evaluated in field assays and with a collection of accessions known to be either susceptible or resistant to tospoviruses. An almost complete correlation was found between resistance under greenhouse/field conditions and the presence of the marker. Therefore, this primer pair is a very useful tool in marker-assisted selection systems in a large range of tomato accessions

Una introgresión asociada con el locus Sw-5 del cromosoma 9 de tomate produce un aumento del contenido de ácido ascórbico en fruta madura. [Resumen]

En tomate han sido identificados y mapeados varios loci de herencia cuantitativa (QTL) relacionados al contenido de ácido ascórbico (AA). Algunos colocalizan con diferentes enzimas relacionadas a las rutas metabólicas de AA. Alelos de interés para el mejoramiento han sido identificados en Solanum pennelli, S. habrochaites y S. lycopersicon var cerasiforme

Expresión fenotípica, herencia y localización cromosómica de mtz: locus recesivo que aporta altos niveles de tolerancia a metribuzin en tomate. [Resumen]

El metribuzin es un herbicida ampliamente usado en cultivos de tomate (Solanum lycopersicon) a campo, ya sea para mesa o procesamiento. Los productores generalmente lo utilizan en pre-trasplante o en etapas tempranas del desarrollo del cultivo. Esta información será útil para futuros esfuerzos en identificar candidatos para la identidad genética del locus mtz

Pepper yellow mosaic virus, a new potyvirus in sweet-pepper. Archives of Virology

A potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil. The sequence analysis of the 3' terminal region of the viral RNA revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3'-untranslated region preceding a polyadenylated tail. The virus shared 77.4% coat protein amino acid identity with Pepper severe mosaic virus, the closest Potyvirus species. The 3'-untranslated region was highly divergent from other potyviruses. Based on these results, the virus found in sweetpepper plants could be considered as a new potyvirus. The name Pepper yellow mosaic virus (PepYMV) is suggeste

Alelo con pérdida de función de un gen tipo TAC-1 (SlTAC1) localizado en el cromosoma 10 de tomate es candidato para el mutante Hoja Erecta (Erl). [Resumen]

La base genética del fenotipo hoja erecta fue investigada en distintas poblaciones, incluida la derivada de Solanum lycopersicum 'LT05' (con hoja erecta y maduración uniforme, genotipo uu) × S. pimpinelifollium 'TO-937' (con fenotipo de hoja salvaje o normal y fruta con hombros verdes, genotipo UU)

Pepper yellow mosaic virus, a new potyvirus in sweet-pepper. Archives of Virology

A potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil. The sequence analysis of the 3' terminal region of the viral RNA revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3'-untranslated region preceding a polyadenylated tail. The virus shared 77.4% coat protein amino acid identity with Pepper severe mosaic virus, the closest Potyvirus species. The 3'-untranslated region was highly divergent from other potyviruses. Based on these results, the virus found in sweetpepper plants could be considered as a new potyvirus. The name Pepper yellow mosaic virus (PepYMV) is suggeste