Impaired Deoxyribonuclease I Activity in Patients with Inflammatory Bowel Diseases

Background and Aims. Deoxyribonuclease I (DNaseI) is an endonuclease that facilitates chromatin breakdown and promotes susceptibility to autoimmune disorders. The aim of current study was to investigate serum DNase I activity in patients with inflammatory bowel diseases (IBD). Patients and Methods. A cohort of 110 IBD patients was evaluated, aged 35 ± 12 years, 77 with Crohn's disease (CD) and 33 with ulcerative colitis (UC). 50 SLE patients and 50 healthy blood donors were examined as control groups. Results. DNase I activity in IBD patients was significantly lower than in healthy individuals, but higher than in SLE patients (P < .0001). Patients with UC showed higher DNase I activity than CD patients, P = .21. DNase I activity in female patients with IBD was significantly lower than in males, P = .024; however, no differences in DNase I activity were found in relation to gender in healthy individuals. DNase I activity has shown a strong negative correlation with the serum concentration of anti-nucleosomal antibodies in the autoimmune (SLE + IBD) cohort, as well as in the separate IBD cohort. Conclusions. Reduced serum DNase I activity probably has pathogenetic consequences in IBD. Induction of autoantibodies towards nucleosomes could be a reflection of impaired DNase I activity

Analýza MHD vybavených měst na severní Moravě a jejich kriteriální posouzení

Import 11/05/2012Prezenční227 - Katedra dopravního stavitelstvíNeuveden

Carcinogenesis of colorectal cancer and cancer associated with inflammatory bowel diseases: theoretical basis and its implications for clinical practice

Colorectal cancer originates as a consequence of accumulating genetic changes and sequential conversion from normal epithelium through dysplastic, intraepithelial neoplasia. It is called an adenoma in subjects with sporadic cancer and dysplasia in those with ulcerative colitis. An inactivation of the APC gene in former and p53 gene in the later group was found to be an initial genetic event in most cases. However, in case of sporadic adenoma, some data indicate the possibility that mutation of only one APC allele occurs early at the stage of adenoma, while the loss of the second allele may occur later, at the time of adenoma to carcinoma progression. Epigenetic changes, namely hypermethylation of the gene promoter, play an important role during carcinogenesis of both sporadic and UC related types of neoplasia. Recent epidemiologic studies suggest the relative risk for UC associated colorectal cancer varies between 2-3. Besides risk factors already recognized, i.e. disease extension and duration, and coincidental primary sclerosing cholangitis, the severity of inflammation and presence of structural changes in the bowel substantially affect the risk for neoplastic conversion. Using the current technique, most dysplastic lesions in IBD patients are nowadays detectable endoscopically. Also, their endoscopic..

Vzdělávání zaměstnanců ve vybrané organizaci

Import 21/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Ekonomická fakulta. Katedra (115) podnikatelství a management

Assessment of risks related to lift assembly and servicing

Import 17/11/2008Diplomová práce řeší problematiku bezpečnosti a ochrany zdraví při práci vycházející z platných legislativních a normativních předpisů. Cílem této diplomové práce je posoudit rizika týkajících se činností při montáži a servisu výtahu, vyhledat rizika nová a navrhnout opatření vedoucí ke snížení těchto rizik.Prezenční040 - Katedra bezpečnostního management

Fecal zonulin is elevated in Crohn’s disease and in cigarette smokers

Objectives: Human zonulin is a protein that increases permeability in the epithelial layer of the small intestine by reversibly modulating the intercellular tight junctions. There is not sufficient information available about zonulin's participation in inflammatory bowel diseases (IBD). The aim of this study was therefore to investigate fecal and serum zonulin in IBD patients and its relation to the disease localization, behavior and smoking status. Design and methods: Forty IBD patients and forty healthy persons were examined for fecal and serum zonulin concentrations by competitive ELISA (DRG International Inc). Values were correlated to IBD type, localization and behavior, and smoking. Results: Serum and fecal zonulin were significantly higher in patients with Crohn’s disease compared to ulcerative colitis (p = 0.038 for fecal zonulin, and p = 0.041 for serum zonulin concentrations). No association of serum or fecal zonulin was found with respect to IBD localization and behavior. The only difference was found with respect to smoking. Both the IBD cohort and healthy smokers showed significantly higher fecal zonulin levels (median 203 ng/mL) compared to non-smokers (median 35.8 ng/mL), p < 0.001. Conclusions: Fecal and serum zonulin levels are elevated in patients with active Crohn’s disease but not with ulcerative colitis. High fecal zonulin levels in smokers irrespective of IBD point to the significant and undesirable up-regulation of gut permeability in cigarette smokers. Keywords: Zonulin, Inflammatory bowel disease, Crohn's disease, Ulcerative colitis, Smokin

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Background and Aims. Deoxyribonuclease I (DNaseI) is an endonuclease that facilitates chromatin breakdown and promotes susceptibility to autoimmune disorders. The aim of current study was to investigate serum DNase I activity in patients with inflammatory bowel diseases (IBD). Patients and Methods. A cohort of 110 IBD patients was evaluated, aged 35 ± 12 years, 77 with Crohn&apos;s disease (CD) and 33 with ulcerative colitis (UC). 50 SLE patients and 50 healthy blood donors were examined as control groups. Results. DNase I activity in IBD patients was significantly lower than in healthy individuals, but higher than in SLE patients (P &lt; .0001). Patients with UC showed higher DNase I activity than CD patients, P = .21. DNase I activity in female patients with IBD was significantly lower than in males, P = .024; however, no differences in DNase I activity were found in relation to gender in healthy individuals. DNase I activity has shown a strong negative correlation with the serum concentration of anti-nucleosomal antibodies in the autoimmune (SLE + IBD) cohort, as well as in the separate IBD cohort. Conclusions. Reduced serum DNase I activity probably has pathogenetic consequences in IBD. Induction of autoantibodies towards nucleosomes could be a reflection of impaired DNase I activity