Case of Ruptured Ectopic Pregnancy in the Uterosacral Ligament and Review of the Literature

Pregnancies that implant on the uterosacral ligament are rare. Here, we describe a case of ruptured ectopic pregnancy in the left uterosacral ligament in a patient with potential risk factors including possible endometriosis and recent hysteroscopic procedure. A 29-year-old female, para 0, presented to the emergency department with generalized abdominal pain. Pelvic examination was significant for fullness in the posterior cul-de-sac. Laboratory values were significant for beta-human chorionic gonadotropin (hCG) level of 6311 mIU/mL. Sonogram findings were significant for no intrauterine gestation, a 6.9×4.6×4.7 cm3 complex left adnexal mass, and moderate free fluid within the posterior cul-de-sac. The patient underwent laparoscopy, which revealed hemoperitoneum and unremarkable bilateral fallopian tubes and ovaries. An abnormal area was noted in the left uterosacral ligament. Tissue was bluntly removed and pathologically confirmed as chorionic villi within the left uterosacral ligament. After one week, her beta-hCG decreased to 784 mIU/mL. After two weeks, she was seen as an outpatient and was doing well without any symptoms. More information is required regarding these unique pregnancies to help understand the pathophysiology and determine the management

Bacteriological profile and antimicrobial susceptibility patterns of symptomatic bloodstream infection in Dhaka City

INTRODUCTION: Bacteremia, is regarded as a significant reason in all over the world for morbidity and mortality in mainly sick patients. Invasion by microorganisms in blood constitutes the critical issues in infectious disease. The aims and objectives are to find out the pathogenic agents infecting males and females in blood of different age groups and naturally used antimicrobial sensitivity profiles. METHODS: All the blood samples were collected aseptically and tested in Popular Diagnostic Centre, Dhaka, Bangladesh between May, 2020 and October, 2020. Total 305 blood specimens from both sexes of different age groups were collected. Cultural analysis was performed by Gram staining, phenotypic examination, biochemical and serological analysis. Finally, antibiotic susceptibility test was done against commonly used antibiotics. RESULTS: 96 (31.47%) samples yielded a positive culture with predominant isolate being Salmonella typhi (41.66%)  though Salmonella spp., Salmonella typhi, Staphylococcus spp and Pseudomonas spp. were also detected. Like most of previous reports, Salmonella spp was predominant, this corroborates this study. But the profile of antimicrobial susceptibility of the detected organisms varied comparing studies which were done in the past. The isolates were found mostly resistant to nalidixic acid. Most of the pathogens showed tremendous susceptibility against ceftriaxone, cefixime, ceftazidime etc. CONCLUSION: The antibiotic selection for the treatment of bacteraemia in patients should always be serious concern due to multidrug resistant (MRD) bacterial isolates. For proper treatment of anti-bacterial resistance and critical mortality and morbidity should be related with the sickness. For validating more reliability, this research requires further work

Gamma Secretase Inhibitors as Potential Therapeutic Targets for Notch Signaling in Uterine Leiomyosarcoma

Uterine leiomyosarcoma (uLMS) is a rare and aggressive cancer with few effective therapeutics. The Notch signaling pathway is evolutionarily conserved with oncogenic properties, but it has not been well studied in uLMS. The purpose of our study was to determine expression of Notch family genes and proteins and to investigate the therapeutic effect of γ-secretase inhibitors (GSIs), indirect inhibitors of Notch signaling, in uLMS. We determined expression of Notch genes and proteins in benign uterine smooth muscle tissue, fibroids, and uLMS samples by immunostaining and in two uLMS cell lines, SK-UT-1B (uterine primary) and SK-LMS-1 (vulvar metastasis) by RT-PCR, Western blot and immunostaining. We exposed our cell lines to GSIs, DAPT and MK-0752, and measured expression of HES1, a downstream effector of Notch. Notch proteins were differentially expressed in uLMS. Expression of NOTCH3 and NOTCH4 was higher in uLMS samples than in benign uterine smooth muscle and fibroids. Expression of NOTCH4 was higher in SK-LMS-1 compared to SK-UT-1B. Exposure of SK-UT-1B and SK-LMS-1 to DAPT and MK-0752 decreased expression of HES1 and decreased uLMS cell viability in a dose- and time-dependent manner that was unique to each GSI. Our findings suggest that GSIs are potential therapeutics for uLMS, albeit with limited efficacy

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background: Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods: The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18-49, 50-69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results: NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion: As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population