Nuclear receptor expression patterns in murine plasmacytoid and conventional dendritic cells

Abstract

Item does not contain fulltextDendritic cells (DC) play a central role in the immune system. They can either induce immunity or promote tolerance. The DC family is generally comprised of two functionally distinct DC subsets. Conventional dendritic cells (cDC) are the classical antigen presenting cells; plasmacytoid dendritic cells (pDC) are the main producers of type I interferons thereby serving innate immunity. Upon activation DCs are able to present antigen and stimulate T cells. The immune modulatory functions of DCs largely depend on the recognition of soluble cues. Besides pathogen derived cues, recent data indicate that the tissue micro-environment, i.e. of the gut and skin affects cDC function. Many of these micro-environmental factors are ligands for the nuclear receptor (NR) family of transcription regulators known to affect immunity and tolerance. Whether pDC function is also influenced by tissue derived cues, like hormones, vitamins and metabolic products, is largely unknown. Here, we investigated the NR expression profile of murine pDCs and cDCs. We assessed the mRNA levels of 19 NRs of in vitro derived as well as ex vivo isolated DCs from four different lymphoid tissues. We observed that cDCs and pDCs expressed the same repertoire of NRs. Expression levels, however, differed between the two subsets, especially upon maturation of DCs. These data imply that NR ligands do impact pDC function and that their activity might be regulated in a DC-specific manner