The aim of this study was to discuss the level of progesterone and prolactin during the development of normal pregnancy and determine the report of progesterone and prolactine level with pregnant women and non-pregnant women. Material and methods: In this study are included 60 women with normal pregnancy (group N) and 60 non-pregnant women (groups S). In both groups blood samples were taken from cubital veins and determination of progesterone and prolactin concentration. Group N (n=60) was selected in prospective way by eliminating from the study all the patients with pregnancy pathology. Group S (n=60) was selected in prospective way by eliminating from the study the pathology of endocrine system. Results: First results showed that the level of progesterone in significant way is raised during the pregnancy (t=t=2.589, P=0.0406 p\u3c 0.05). Prolactin is raised in significant way during the development of pregnancy. (t=3.174, p 0.0252, p\u3c0.05 ). While comparing the progesterone values with pregnant and non-pregnant women, we have ascertained that the level of progesterone in significant way was higher with pregnant women (q=10.707, p\u3c0.001). While comparing the prolactin values with pregnant and non-pregnant women, we have ascertained that the level of prolactin in significant way was higher with pregnant women (q=11.451, P \u3c 0.001). Discussion: The level of progesterone is raised due to development of placenta during pregnancy and increase of fetal area of suprarenal gland of fetus. The level of prolactin during pregnancy is raised due to effective stimulated placental steroids, estrogens, and rilizing tiretrop hormone in hypothalamus on one side and due to presence of prolactin decidul during pregnancy. Conclude: We conclude that the level of prolactin and progesterone is raised in significant way during the development of pregnancy. The values of progesterone and prolactin at pregnancy are raised in significant way comparing with non-pregnant women