Isolation and preliminary characterization of a new bacteriophage against Sphaerotilus natans

Abstract

Activated sludge process, the most commonly used system for biological wastewater treatment, is driven by a community of microorganisms that play a key role in the conversion of the organic matter and removal of nutrients from wastewater. In this artificial ecosystem, filamentous and floc-forming bacteria co-exist in a dynamic equilibrium. However, filamentous bacteria overgrowth lead to a decrease of performance of the plant by causing two well-known phenomena: bulking and/or foaming. Sphaerotilus natans is a filamentous bacterium that can cause or contribute to the malfunction of these systems by leading to bulking occurrence. Bacteriophages are regarded as possible novel treatment against the filamentous overgrowth. In this work, the isolation and preliminary characterization of a new lytic bacteriophage against S. natans are described. Results clearly indicate the potential effect of this bacteriophage for future wastewater treatment system management.Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), the project INNOVCONTROLFIL (PTDC/AAG-TEC/3331/2014), the Grant SFRH/BPD/73720/2010 to Susana Costez and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norteinfo:eu-repo/semantics/publishedVersio

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