Methanolic (70%) extracts of marigold flower, leaf, stem and root, after solvent removal, were successively extracted with ether, chloroform, ethyl acetate, and n-butanol - the residue being the corresponding aqueous extract. All five extracts were evaporated to dryness and then dissolved in 50% EtOH to obtain 6% (w/v) solutions. These solutions, either alone or in combination with different doses of ciprofloxacin or pyralene, were used to study their effect on liposomal lipid peroxidation (LPx) induced by Fe 2+ and ascorbic acid. The majority of the extracts of marigold flower, leaf and stem showed an antioxidant effect, the butanol extract of roots exhibiting a pro-oxidative character. At lower doses, ciprofloxacin did not affect LPx, at higher doses a dose-dependent increase in LPx was observed. The extracts in combination with ciprofloxacin showed a protective effect, which was also dose-dependent. The butanolic extract of root snowed no synergistic effect with ciprofloxacin. Pyralene alone exhibited a pro-oxidative action on liposomal LPx. Aqueous and butanolic extracts of flower, leaf, and stem had a protective effect in combination with pyralene. The butanolic extract of root showed a pro-oxidative synergistic effect with pyralene
