探讨了利用固定床反应器进行脂肪酶催化合成维生素A棕榈酸酯的可行性．利用HPD826吸附法获得固定化酶活力达到9200U／g，确定了反应介质为石油醚，进料流量8mL／min，反应温度为35℃，维生素A乙酯及棕榈酸的浓度分别为3．6g／L和14g／L．在此优化条件下反应，转化率可达80％，维生素A棕榈酸酯的生产能力为281g／（L·h）．反应器连续运行12h，转化率维持在68％以上．结果表明，利用固定床反应器进行酶促合成维生素A棕榈酸酯可行，可进一步深入研究．The feasibility of using fixed bed reactor for synthesis of vitamin A palmitate catalyzed by li- pase was investigated in this work.First, the lipase was immobilized by adsorption with HPD826 as car- rier and give the activity of 9200 U/g.Then the appropriate reaction conditions were determined：the re- action media is petroleum ether,the feed flow rate is 8 mL/min,the temperature is 35 ℃ ,the concen- trations of vitamin A acetate and palmitic acid are 3.6 and 14 g/L.Under these conditions,the conver- sion and productivity of vitamin A palmitate can reach 80% and 281 g/（L · h） ,respectively.Finally,the reactor was continuously run for 12 h,the conversion held upon 68%.These results showed that the synthesis of vitamin A palmitate by immobilized lipase in fixed bed reactor was feasible and worth fur- ther investigation.厦门市科技计划项目（3502220143008
