Bao, Aike

Wang, Suomin

Wu, Guoqiang

Xi, Jiejun

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Molecular Cloning and Characterization of a Vacuolar Na+/H+ Antiporter Gene from the Succulent Xerophyte Zygophyllum xanthoxylum Guoqiang Wu Lanzhou University, China Jiejun Xi Lanzhou University, China Aike Bao Lanzhou University, China Suomin Wang Lanzhou University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-2/21 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]402　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsMolecular cloning and characterization of a vacuolar Na＋ /H＋ antiporter gene from the succulentxerophyte Zygophyllum xanthoxylumGuo‐Qiang W u , Jie‐Jun X i , A i‐Ke Bao , Suo‐Min W ang 倡Key L aboratory o f G rassland A gro‐ecosystem , M inistry o f A griculture ,School o f Pastoral A griculture Science and Technology , L anz hou University , L anz hou 730000 , Peop le摧s Republic o f China .倡 Corresponding author E‐mail : smwang＠ lz u .edu .cnKey words :Xerophyte ,vacuolar Na ＋ / H ＋ antiporter ,ZxN HXIntroduction Salinity is an environment factor that adversely affects plant grow th and development . High salinity causes osmoticand oxidative stresses , and the damage in ion homeostasis . To cope with salt stress , plants have developed a variety ofadaptation mechanisms . One of them is to compartmentalize Na ＋ into the vacuole through the operation of a vacuolar Na ＋ / H ＋antiporter ( NHX) ( Apse et al . , １９９９ ) . So far , NHX genes have been isolated from the glycophytes , such as A tN HX1 inA rabidopsis thaliana ,OsN H X1 in rice and GhN HX1 in cotton , and the halophytes , such as SsN HX in Suaeda salsa andSeN HX1 in Salicornia europaea . However , NHX genes from the xerophytes have been not reported . In this study , ahomologous gene ( ZxN HX ) of vacuolar Na＋ / H ＋ antiporter was cloned and characterized from a typically succulent xerophyte ,Zygophy llum xanthoxylum .Materials and methods The core fragment of ZxN HX was cloned by RT‐PCR . The full‐length cDNA ends of ZxN HX wereisolated by RACE system . Sequence analysis was run on the sof tware DNAstar . Homology comparison was analyzed usingDNAMAN software .Results The full‐length cDNA of vacuolar Na ＋ / H ＋ antiporter gene from Z . xanthoxy lum was obtained by assembling the corecDNA fragment ( Figure １ a) , ５�‐RACE fragment ( Figure １ b) and ３�‐RACE fragment ( Figure １ c) . This cDNA consists of２１２７ bp with a ５�‐untranslated region of ２１３ bp , an open reading frame of １５５８ bp that encodes a protein of ５３２ amino acidswith a calculated molecular weight of ５８ .８ KDa , and a ３�‐untranslated region of ３１５ bp containing a ploy ( A ) tail . It wasnamed as ZxN HX and deposited in the GenBank database ( accession no . EU１０３６２４ ) . The deduced amino acid sequence ofZxN HX shares a high homology with those of the vacuolar Na＋ / H ＋ antiporters of higher plants , ranging from ８０ .７％homology with that of Populus euphratica PeNHX２ to ７２ .７％ with that of Oryz a sativ a OsN HX１ , and contains the consensusamiloride‐binding domain . Semi‐quantitative RT‐PCR analysis showed that the expression of ZxN HX in Z . xanthoxy lum wasinduced and regulated by salt stress .Figure 2 A garose gel electrophoresis o f the core f ragment and RACE f ragments o f ZxN HX .Conclusions The results indicated that the gene ZxN HX isolated from Z . xanthoxy lum is a vacuolar type Na ＋ / H ＋ antiporters .This gene may play important roles in the drought tolerance , its mechanism is under further investigation .ReferenceApse MP , Aharon , GS , Snedden WA , Blumwald E . １９９９ . Salt tolerance conferred by overexpression of a vacuolar Na ＋ / H ＋antiporter in A rabidopsis .Science . ２８５ : １２５６‐１２５８ .

Molecular Cloning and Characterization of a Vacuolar Na\u3csup\u3e+\u3c/sup\u3e/H\u3csup\u3e+\u3c/sup\u3e Antiporter Gene from the Succulent Xerophyte \u3ci\u3eZygophyllum xanthoxylum\u3c/i\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3326&amp;context=igc

Molecular Cloning and Characterization of a Vacuolar Na\u3csup\u3e+\u3c/sup\u3e/H\u3csup\u3e+\u3c/sup\u3e Antiporter Gene from the Succulent Xerophyte \u3ci\u3eZygophyllum xanthoxylum\u3c/i\u3e

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