Li, Xiaolei

Li, Zaozhe

Ma, Yanhong

Yu, Zhuo

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings XXI International Grassland Congress / VIII International Rangeland Congress Identification of Chromosome and Fertility of BC1 of Pentaploid Hybrid between Elymus canadensis and Elymus dahuricus Zhuo Yu Inner Mongolia Agricultural University, China Yanhong Ma Inner Mongolia Agricultural University, China Xiaolei Li Inner Mongolia Agricultural University, China Zaozhe Li Inner Mongolia Agricultural University, China Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/12-2/29 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 瞯 ]410　 瞯 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅱ 　Grasslands/Rangelands Production Systems——— Developing Improved PlantsIdentification of chromosome and fertility of BC1 of pentaploid hybrid between Elymuscanadensis and Elymus dahuricusYu Zhuo , Ma Y an‐hong , L i X iao‐lei , L i Zao‐z heA gronomy College ,Inner Mongolia A gricultural University ,Hohhot ,Inner Mongolia 010019 ,China . E‐mail : yuz huo58＠sina .comKey words :Elymus canadensis ,Elymus dahuricus ,BC１ , chromosome ,fertilityIntroduction In recent years , the artificial wide cross between Elymus canadensis (２n ＝ ４x ＝ ２８) and Elymus dahuricus (２n ＝ ６x＝ ４２) was conducted and the interspecific hybrid F１ was obtained successfully . It is reported that the hybrid F１ is pentaploid ( ２n＝ ５x ＝ ３５) , and highly sterile . In order to restore fertility to hybrid F１ , hybrid F１ was backcrossed by recurrent parent E .canadensis and BC１ plant was obtained . The chromosome number and pollen fertility , seed set of BC１ plant were identified inthis test in order to find out the cytogenetic characters and status of fertility restoration and to provide scientific basis forselection and utilization of excellent lines of cross descendents .Materials and methods The tested materials were BC１ plant of hybrid F１ between E . canadensis and E . dahuricus , which wasbackcrossed by recurrent parent E . canadensis .The RTC chromosome of BC１ was observed by improved fuchsine stainingmethod ; the pollen fertility was observed by acetic carmine staining method ; ３０ ears of BC１ population were taken randomly inseed maturing stage , seeds and florets were counted up , seed set ＝ ( Total seeds/ Total florets observed) × １００％ .Results and analysis Figure １ showed that the RTC chromosome number of BC１ varied , ranging from ２１ to ３５ , and the RTCchromosome number are ２８ in above ８５％ of ５１５ cells . Results showed that the RTC chromosome number of BC１ tend torecurrent parent E . canadensis , however the partial chromosome of pentaploid hybrid was lost , and the further research areneeded . The fertility observation results of BC１ plant showed that ２５２４ pollen were fertile in ３１３０ pollen , and the rate of pollenfertility reached ８０ .６４％ ; １９０５ florets seeded in ２８００ florets , and seed set was ６８ .０４％ . Hence it was proved that the fertilityof BC１ plant had been restored via one time backcross , and this result can provide scientific basis for selection and utilization ofexcellent lines of cross descendents .　 Figure 1 RTC chromosome o f [ E . canadensis × E . dahuricus F1 ] × E . canadensis BC1 p lanta ,b ,c ,d ,e . RTC chromosome number o f BC1 2n ＝ 28 ; f . 2n ＝ 21 ; g . 2n ＝ 25 ; h .i . 2n ＝ 26 ; j . 2n ＝ 29 ; k . 2n ＝ 35 ; 1 . 2n ＝ 31 .Conclusions The RTC chromosome number of BC１ varied ranging from ２１ to ３５ , in which the chromosome number of most cells( above ８５％ of cells) are ２８ , the RTC chromosome number of BC１ tend to tetraploid E . canadensis , the effect of backcross issignificant ; the pollen fertility and the seed set of BC１ are higher , ８０ .６４％ and ６８ .０４％ , respectively , the fertility of BC１ planthad been obviously restored .ReferenceYU Zhuo , SONG Yongfu , LI Zaozhe , et al . ２００２ . Study on the grow th , development and cytogenetics of Elymus canadensis× E . dahuricus hybrid F１ [ J] . A cta A grestia Sinica , １０(４) : ２５８‐２６４ .

Identification of Chromosome and Fertility of BC1 of Pentaploid Hybrid between \u3ci\u3eElymus canadensis\u3c/i\u3e and \u3ci\u3eElymus dahuricus\u3c/i\u3e

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=3334&amp;context=igc

Identification of Chromosome and Fertility of BC1 of Pentaploid Hybrid between \u3ci\u3eElymus canadensis\u3c/i\u3e and \u3ci\u3eElymus dahuricus\u3c/i\u3e

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