En, Wu

Sugawara, K.

Xin, G. R.

English

University of Kentucky

University of Kentucky UKnowledge International Grassland Congress Proceedings 21st International Grassland Congress / 8th International Rangeland Congress Growth and P Uptake of Dactylis glomerata L . and Anthoxanthum odoratum L . Response to Mycorrhizal Inoculation in Acid Condition Wu En Inner Mongolia Agricultural University, China G. R. Xin Sun Yat-sen University, China K. Sugawara Tohoku University, Japan Follow this and additional works at: https://uknowledge.uky.edu/igc  Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/3-2/41 The 21st International Grassland Congress / 8th International Rangeland Congress took place in Hohhot, China from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House This Event is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in International Grassland Congress Proceedings by an authorized administrator of UKnowledge. For more information, please contact UKnowledge@lsv.uky.edu. 　 Multifunctional Grasslands in a Changing World 　 Volume Ⅰ 　 瞯 ]527　 瞯Grasslands/Rangelands Resources and Ecology ——— Soil‐Plant‐Animal InterrelationshipsGrowth and P uptake of Dactylis glomerata L . and Anthoxanthum odoratum L . response tomycorrhizal inoculation in acid conditionWu En1 ,3 , G .R . X in 2 ,3 , K .Sugaw ara31 College o f Ecol . and Env . Sci . , Inner Mongolia A gric . Univ . , H uhhot , Inner Mongolia 010018 P .R . o f China ; 2 Collegeo f L i f e Sci . , Sun Y at‐sen Univ .Guang z hou , Guang dong 510275 P .R . o f China ; 3 College o f A gric . Sci . , Tohoku Univ . ,Sendai 981‐0914 P .R . o f Japan . 1 E‐mail :wuen2004＠ 163 .comKey words : acid condition , A nthoxanthum odoratum L . , mycorrhizal inoculation , Dacty lis glomerata L . , responseIntroduction We found the AMF colonization of A .odoratum not correlated with soil pH , and coverage of A .odoratum stronglycorrelated with AMF colonization in our field study (Wu et al . ２００６) . In addition , we also found the mycorrhizal colonization ,total spore number in rhizosphere of A .odoratum prominently higher than that of the D .glomerata , respectively . The sporedensity of A .odoratum and D .glomerata were of １５ and ４ spores per １０g fresh soil , respectively . Four different morphologicaltypes of spores occurred in rhizosphere of A .odoratum and only one morphological type of spore occurred in rhizosphere of D .glomerata . These results suggest that the A .odoratum invade and extend in severity acidic pasture origin of this specificity onarbuscular mycorrhizal symbiosis in acidic soil other than D .glomerata . In present study , we explored the effect of soil pH inmycorrhizal colonization , P uptake and grow th of both plants by glasshouse experiment .Materials and methods Plant materials : D .glomerata , is grass ; A .odoratum , is weed . T reatment and soil modulate : allexperiment include ２ ( soil sterilized and non sterilized ) x ４ ( pH ＝ ３ , ４ ( control ) , ５ , ６ ) ＝ ８ treatments . Cultivating soilcollected from an acidic artificial pasture ( pH ＝ ４) , soil sterilized by benomyl ( Fitter and Nichols １９８８) , soil pH modulated byNaOH . Planting and harvest : ４ seedlings of D .glomerata and A .odoratum mixed planting to per pot respectively at ２ Jun２００３ , all treatment harvested at ２ September ２００３ . Measures : The roots were stained with trypan blue / lactic acid and themycorrhizal colonization ( percentage of root infected by arbuscular mycorrhizal fungi) was calculated using the line‐intersectmethod ( Giovannettti and Mosse １９８０ ) . Shoot biomass and P content measured after drying at ７０ ℃ with ４８h . Experimentconducted in farm of college of Agricultural Science of Tohoku University , Osaki , Miyagi prefecture , Japan (３８°４５′N ,１４０°４５′E) .Results Significantly positive correlation occurred in soil pH between Mycorrhizal colonization of D .glomerata , but the A .odoratum was not yet . A significantly positive correlation occurred in mycorrhizal colonization between shoot dry mass of bothplants . Also a significantly positive correlation occurred in mycorrhizal colonization between shoot P content of both plants( Table １) .Table 1 Regression equations o f some parameters o f D .glomerata and A .odoratum .Plants regression equationsD .glomerata y１ ＝ １０ -.０７x１‐２４ .７０ ,r ＝ ０ .８９４ ,p ＜ ０ .０００１ y２ ＝ ０ 适.０１x２ ＋ ０ .２３ ,r ＝ ０ .８６７ ,p ＝ ０ .０００３ y３ ＝ ０ 晻.０４x２ ＋ ２ .３２ ,r ＝ ０ .７４８ ,p ＝ ０ .００５A .odoratum y１ ＝ １ �.８０x１ ＋ ２０ .０５ ,r ＝ ０ .２４３ ,p ＝ ０ .４４６ y２ ＝ ０ 适.０１x２ ＋ ０ .２１ ,r ＝ ０ .７７８ ,p ＝ ０ .００２９ y３ ＝ ０ 晻.０７x３ ＋ １ .６１ ,r ＝ ０ .７９７ ,p ＝ ０ .００２y１ Mycorrhizal colonization , x１ soil pH , y２ shoot dry mass , x２ Mycorrhizal colonization , y３ shoot P content , x３ Mycorrhizal colonization .Conclusions Based on these results , we concluded that the A .odoratum have a specific mycorrhizal symbiosis in acid soilcondition . Mycorrhizal colonization of D .glomerata strongly affected by soil pH , but the A .odoratum was not yet , mycorrhizalcolonization of D .glomerata significantly decreased by declined the soil pH , result to decrease the nutrient uptake , grow th ,acidity tolerance and competition ability of D .glomerata , conduced its declined . On the contrary , mycorrhizal colonization ofA .odoratum not decreased by declined the soil pH , the mycorrhizal symbiosis led to a positive effect on nutrient uptake ,grow th , acid tolerance and competition ability of A .odoratum . Consider to A .odoratum invade and extend in severity acidicartificial pasture origin of this specificity on arbuscular mycorrhizal symbiosis in acidic soil other than D .glomerata . Our resultshave a important meaning and value reference on understand to mechanism of invade and extend in severity acidic artificialpasture by A .odoratum and declined of D .glomerata in same condition .ReferencesWu En , G .R . Xin , K .Sugawara , et al . ( ２００６ ) . Effect of acidic soil on arbuscular mycorhhizal symbiosis of A nthoxanthumodoratum L . Acta agrestia sinica １４ (２) : １５２‐１５５ .Fitter ,A .H . and Nichols ,R . ( １９８８ ) . The use of benomyl to control infection by vesicular‐arbuscular mycorrhizal fungi . Newphytol . １１０ :２０１‐２０６ .Giovannettti M .and Mosse B . (１９８０ ) . An evaluation of techniques for measuring VAM infection in roots . New phytol . ８４ :４８９‐５００ .

Growth and P Uptake of \u3cem\u3eDactylis glomerata\u3c/em\u3e L . and \u3cem\u3eAnthoxanthum odoratum\u3c/em\u3e L . Response to Mycorrhizal Inoculation in Acid Condition

https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=2764&amp;context=igc

Growth and P Uptake of \u3cem\u3eDactylis glomerata\u3c/em\u3e L . and \u3cem\u3eAnthoxanthum odoratum\u3c/em\u3e L . Response to Mycorrhizal Inoculation in Acid Condition

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