학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과, 2016. 2. 신종헌.효소 sortase A에 대해 저해 생리활성을 가지는 천연 물질을 찾고자 다양한 생약 추출물을 평가하였다. 생리활성 검색 결과에 따라 계혈등을 연구대상으로 선택하였는데 이는 혈액순환 개선과 생리통, 빈혈, 마비, 관절통 및 세균 감염에 사용되는 전통 생약이다. Sortase A에 다하여 높은 저해활성을 보이는 계혈등 추출물의 분획에 대하여 다양한 크로마토그래피 분리 기법으로 총 20개의 물질을 분리하였다. 복합적 분광학적 분석의 결과를 토대로 분리된 물질들이 18종의 flavonoids, 1종의 phenolic, 1종의 flavonoid dimer 임을 동정하였다. 이들 중 3종의 flavonoid 계열 물질이 계혈등에서는 처음 발견된 물질임을 확인하였다.For the investigation of bioactive natural products with sortase A (SrtA) inhibitory effect, various extracts of Korean herbal medicines were evaluated. Based upon the results of bioactivity screening, the dried vine stem of Spatholobus suberectus Dunn. which is an oriental folk medicine used mainly for the improvement of blood circulation and treatment for dysmenorrhea, anemia, paralysis, arthralgia and bacterial infections was selected for chemical investigation. The large-scale extraction followed by the bioactivity-guided partition and chromatographic separation yielded twenty compounds. Based upon the results of combined spectroscopic analyses, these compounds were structurally identified as eighteen flavonoids, one flavonoid dimer and a phenolic compound. Among these three flavonoids were found from this plant for the first time.Introduction 1
Experimental Section 3
1. General Experimental Procedures 3
2. Plant Material 3
3. Extraction and Isolation 3
Results 7
1. Compound 1 7
2. Compound 2 7
3. Compound 3 8
4. Compound 4 9
5. Compound 5 9
6. Compound 6 10
7. Compound 7 10
8. Compound 8 11
9. Compound 9 12
10. Compound 10 12
11. Compound 11 13
12. Compound 12 13
13. Compound 13 13
14. Compound 14 14
15. Compound 15 14
16. Compound 16 14
17. Compound 17 15
18. Compound 18 15
19. Compound 19 16
20. Compound 20 16
Discussion 38
References 39
Abstract in Korean 52Maste
