<b style="">Benzyl amino purine and adenine sulphate induced rapid multiple shoot and root induction from nodal explants of <i style="">Brahmi</i> [<i style="">Bacopa monnieri </i>(Linn.)Penn.]</b>

Abstract

44-51In vitro multiple shoot and root induction of Brahmi [Bacopa monnieri (Linn.) Penn.] was achieved from nodal explants. Shoot segments obtained from field grown plants were surface sterilized and cultured on Murashige and Skoog’s (MS) medium without any hormones to get regular supply of node explants. In order to find out the suitable conditions for propagation from nodal explants, MS medium with different combinations of auxins and cytokinins were tested. All the treatments were found effective in inducing shoots except those with auxin/cytokinin alone. The percent response of 92-100 was observed in 90% of treatments. The effect of special cytokinin like adenine sulphate on multiple shoot formation from the nodal explants of B. monnieri was tested on MS medium fortified with different hormonal concentrations of auxin and cytokinin. High percentage of multiple shoot formation (96.3) and maximum number of shoots (16.33) was observed in the combinational treatment of IAA (0.2 mg/l) and BAP (1.5 mg/l). Addition of adenine sulphate at 60mg/l along with IAA (0.2mg /l) and BAP (1.5mg/l) was found most effective in inducing highest number of shoots (18). All the other tested combinations have little effect on increasing the number of shoots. In vitro propagated plants were successfully transplanted to plastic pots containing sterile soil with 85% survival rate and later established under ex vitro condition