Aims: The quantitative measurement and characterization of
microscopical images using a computer is histomorphometry. The purpose
of this experimental research is to evaluate the validity of two
methods of histomorphometry of root structure loss measuring
antiresorptive efficacy of topical application of alendronate in
delayed tooth replantation on a canine model. Methods: We used
fortyeight premolar mature roots from five dogs in the study. Roots
were soaked in: 1 mMol of ALN, 3 mMol of ALN and physiological saline.
After 4 months, animals were sacrificed and a bone block was prepared
for analysis. Histological slides were photographed with digital camera
(Olympus C 5060 5.1 megapixel) which is directly attached to microscope
(Carl Zeiss, Axiolab, Jena, Germany) connected to a PC. The images
obtained were stored as figures (TIFF) for further interpretation. Root
structure loss due to resorption was measured in two ways. Results: The
t-test for two independent samples for evaluating root mass loss
between different methods of measurement was used. (t=4.497; df=17;
p<0.05). Conclusion: The obtained results showed additional
accuracy and precision by computer method that enables future
researchers in the area of traumatic injuries of dento-alveolar system,
for applying in pathohistology scientific approach, and for measuring
the resorption changes on the roots of replanted teeth
