Ford JL

Green JB

Green MH

Lietz G

Oxley A

\ua9 2018 The vitamin A value (bioefficacy) of provitamin A carotenoids is determined by absorption of the carotenoid (bioavailability) and its subsequent conversion to retinol (bioconversion). Here we show that intestinal bioconversion of β-carotene can be estimated based on analysis of a single plasma sample collected 6 h after subjects ingest a test dose of stable isotope-labeled β-carotene from the ratio of retinyl esters to retinyl esters plus β-carotene. Plasma isotope ratio predictions of bioconversion ranged from 50 to– 93% (mean 76%) for 45 healthy young adults with low vitamin A stores. Results were the same as predictions made by a traditional area-under-the-curve method calculated from 0 to– 8 h or a modified area-under-the-curve method calculated from 0 to– 12 h. The modified method may provide better estimates of bioconversion between 8 and 24 h after ingestion of a carotenoid dose when stable isotopes cannot be used due to cost or logistics. Furthermore, because the plasma isotope ratio method requires only one blood sample and no isolation of triglyceride-rich lipoproteins, its use will facilitate estimation of provitamin A carotenoid bioconversion in human subjects and especially children, in whom repeated blood sampling is not feasible

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Intestinal β-carotene bioconversion in humans is determined by a new single-sample, plasma isotope ratio method and compared with traditional and modified area-under-the-curve methods

https://eprint.ncl.ac.uk/file_store/production/250194/93D9BBE1-D92B-4ED2-A772-F62925D9665D.pdf

Intestinal β-carotene bioconversion in humans is determined by a new single-sample, plasma isotope ratio method and compared with traditional and modified area-under-the-curve methods

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