The essential oil of Ocimum basilicum L. grown in Republic of Belarus was analyzed by means of GLC. More than 10 compounds were identified in essential oil of Ocimum basilicum L. The representative and characteristic components were linalool, methylchavicol, 1,8-cineole, camphor, ?and ?-pinene, ?-terpineol, eugenol, ?-caryophyllene. The methods of plant material preparation and long-term storage of essential oil influence on component composition