Multispectral and hyperspectral imaging are efficient methods of measuring spectral reflectance at high spatial resolution. This non-invasive technique has been applied to the imaging of paintings over the last 20 years. PRISMS (Portable Remote Imaging System for Multispectral Scanning) was designed specifically for imaging wall paintings. Optical Coherence Tomography (OCT) is a low coherence interferometric technique capable of fast non-invasive imaging of subsurface microstructure. This paper shows the first application of in situ OCT imaging of a wall painting. The combination of PRISMS and OCT gives information on the varnish and paint layer structure, pigment identification, the state of degradation of the paint and varnish layers and informing curators on the painting schemes and techniques